This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Della Valle, G Articles by Basilico, C Search for Related Content PubMed PubMed Citation Articles by Della Valle, G Articles by Basilico, C

Cell transformation mediated by chromosomal deoxyribonucleic acid of polyoma virus-transformed cells.

Department of Pathology, New York University School of Medicine, New York 10016.

ABSTRACT

To study the mechanism of deoxyribonucleic acid (DNA)-mediated gene transfer, normal rat cells were transfected with total cellular DNA extracted from polyoma virus-transformed cells. This resulted in the appearance of the transformed phenotype in 1 X 10(-6) to 3 X 10(-6) of the transfected cells. Transformation was invariably associated with the acquisition of integrated viral DNA sequences characteristic of the donor DNA. This was caused not by the integration of free DNA molecules, but by the transfer of large DNA fragments (10 to 20 kilobases) containing linked cellular and viral sequences. Although Southern blot analysis showed that integration did not appear to occur in a homologous region of the recipient chromosome, the frequency of transformation was rather high when compared with that of purified polyoma DNA, perhaps due to "position" effects or to the high efficiency of recombination of large DNA fragments.

This article has been cited by other articles:

• Zuber, M., Goodman, D., Karns, L., Fishman, M. (1989). The neuronal growth-associated protein GAP-43 induces filopodia in non-neuronal cells. Science 244: 1193-1195 [Abstract]