This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Olsen, A S Articles by Moore, D E Search for Related Content PubMed PubMed Citation Articles by Olsen, A S Articles by Moore, D E

Number and size of human X chromosome fragments transferred to mouse cells by chromosome-mediated gene transfer.

Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20205.

ABSTRACT

Labeled probes of unique-sequence human X chromosomal deoxyribonucleic acid, prepared by two different procedures, were used to measure the amount of human X chromosomal deoxyribonucleic acid in 12 mouse cell lines expressing human hypoxanthine phosphoribosyltransferase after chromosome-mediated gene transfer. The amount of X chromosomal deoxyribonucleic acid detected by this procedure ranged from undetectable levels in the three stable transformants and some unstable transformants examined to about 20% of the human X chromosome in two unstable transformants. Reassociation kinetics of the X chromosomal probe with deoxyribonucleic acid from the two unstable transformants containing 15 to 20% of the human X chromosome indicate that a single copy of these sequences is present. In one of these lines, the X chromosomal sequences exist as multiple fragments which were not concordantly segregated when the cells were selected for loss of hprt.

This article has been cited by other articles:

• Pritchard, C A, Goodfellow, P N (1987). Investigation of chromosome-mediated gene transfer using the HPRT region of the human X chromosome as a model.. Genes Dev. 1: 172-178 [Abstract]