This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pierani, A Articles by Roeder, R G Search for Related Content PubMed PubMed Citation Articles by Pierani, A Articles by Roeder, R G

 Previous Article  |  Next Article 

Mol Cell Biol. 1990 December; 10(12): 6204-6215

Activation of octamer-containing promoters by either octamer-binding transcription factor 1 (OTF-1) or OTF-2 and requirement of an additional B-cell-specific component for optimal transcription of immunoglobulin promoters.

Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.

ABSTRACT

Several distinct octamer-binding transcription factors (OTFs) interact with the sequence ATTTGCAT (the octamer motif), which acts as a transcription regulatory element for a variety of differentially controlled genes. The ubiquitous OTF-1 plays a role in expression of the cell cycle-regulated histone H2b gene as well as several other genes, while the tissue-specific OTF-2 has been implicated in the tissue-specific expression of immunoglobulin genes. In an attempt to understand the apparent transcriptional selectivity of these factors, we have investigated the physical and functional characteristics of OTF-1 purified from HeLa cells and both OTF-1 and OTF-2 purified from B cells. High-resolution footprinting and mobility shift-competition assays indicated that these factors were virtually indistinguishable in binding affinities and DNA-protein contacts on either the H2b or an immunoglobulin light-chain (kappa) promoter. In addition, each of the purified factors showed an equivalent intrinsic capacity to activate transcription from either immunoglobulin promoters (kappa and heavy chain) or the H2b promoter in OTF-depleted HeLa and B-cell extracts. However, with OTF-depleted HeLa extracts, neither factor could restore immunoglobulin gene transcription to the relatively high level observed in unfractionated B-cell extracts. Restoration of full immunoglobulin gene activity appears to require an additional B-cell regulatory component which interacts with the OTFs. The additional B-cell factor could act either by facilitating interaction of OTF activation domains with components of the general transcriptional machinery or by contributing a novel activation domain.

This article has been cited by other articles:

• Laumen, H., Brunner, C., Greiner, A., Wirth, T. (2004). Myosin light chain 1 atrial isoform (MLC1A) is expressed in pre-B cells under control of the BOB.1/OBF.1 coactivator. Nucleic Acids Res 32: 1577-1583 [Abstract] [Full Text]  
• Brunner, C., Laumen, H., Nielsen, P. J., Kraut, N., Wirth, T. (2003). Expression of the Aldehyde Dehydrogenase 2-like Gene Is Controlled by BOB.1/OBF.1 in B Lymphocytes. J. Biol. Chem. 278: 45231-45239 [Abstract] [Full Text]  
• Samardzic, T., Marinkovic, D., Nielsen, P. J., Nitschke, L., Wirth, T. (2002). BOB.1/OBF.1 Deficiency Affects Marginal-Zone B-Cell Compartment. Mol. Cell. Biol. 22: 8320-8331 [Abstract] [Full Text]  
• Malone, C. S., Wall, R. (2002). Bob1 (OCA-B/OBF-1) Differential Transactivation of the B Cell-Specific B29 (Ig{beta}) and mb-1 (Ig{alpha}) Promoters. J. Immunol. 168: 3369-3375 [Abstract] [Full Text]  
• Sharif, M. N., Radomska, H. S., Miller, D. M., Eckhardt, L. A. (2001). Unique Function for Carboxyl-Terminal Domain of Oct-2 in Ig-Secreting Cells. J. Immunol. 167: 4421-4429 [Abstract] [Full Text]  
• Greiner, A., Muller, K. B., Hess, J., Pfeffer, K., Muller-Hermelink, H. K., Wirth, T. (2000). Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am. J. Pathol. 156: 501-507 [Abstract] [Full Text]  
• Luo, Y., Ge, H., Stevens, S., Xiao, H., Roeder, R. G. (1998). Coactivation by OCA-B: Definition of Critical Regions and Synergism with General Cofactors. Mol. Cell. Biol. 18: 3803-3810 [Abstract] [Full Text]  
• Fourel, G., Ringeisen, F., Flajolet, M., Tiollais, P., Buendia, M. A. (1998). Functional Analysis of Ground Squirrel Hepatitis Virus Enhancer II. J. Virol. 72: 1616-1622 [Abstract] [Full Text]  
• Inamoto, S., Segil, N., Pan, Z.-Q., Kimura, M., Roeder, R. G. (1997). The Cyclin-dependent Kinase-activating Kinase (CAK) Assembly Factor, MAT1, Targets and Enhances CAK Activity on the POU Domains of Octamer Transcription Factors. J. Biol. Chem. 272: 29852-29858 [Abstract] [Full Text]  
• Cepek, K L, Chasman, D I, Sharp, P A (1996). Sequence-specific DNA binding of the B-cell-specific coactivator OCA-B.. Genes Dev. 10: 2079-2088 [Abstract]  
• Pfisterer, P., Zwilling, S., Hess, J., Wirth, T. (1995). Functional Characterization of the Murine Homolog of the B cell-specific Coactivator BOB.1/OBF.1. J. Biol. Chem. 270: 29870-29880 [Abstract] [Full Text]  
• Herr, W, Cleary, M A (1995). The POU domain: versatility in transcriptional regulation by a flexible two-in-one DNA-binding domain.. Genes Dev. 9: 1679-1693  
• Konig, H, Pfisterer, P, Corcoran, L M, Wirth, T (1995). Identification of CD36 as the first gene dependent on the B-cell differentiation factor Oct-2.. Genes Dev. 9: 1598-1607 [Abstract]  
• Kristie, T. M., Pomerantz, J. L., Twomey, T. C., Parent, S. A., Sharp, P. A. (1995). The Cellular C1 Factor of the Herpes Simplex Virus Enhancer Complex Is a Family of Polypeptides. J. Biol. Chem. 270: 4387-4394 [Abstract] [Full Text]  
• Schwarzenbach, H., Newell, J. W., Matthias, P. (1995). Involvement of the Ets Family Factor PU.1 in the Activation of Immunoglobulin Promoters. J. Biol. Chem. 270: 898-907 [Abstract] [Full Text]  
• Corcoran, L M, Karvelas, M, Nossal, G J, Ye, Z S, Jacks, T, Baltimore, D (1993). Oct-2, although not required for early B-cell development, is critical for later B-cell maturation and for postnatal survival.. Genes Dev. 7: 570-582 [Abstract]  
• Bray, S J, Kafatos, F C (1991). Developmental function of Elf-1: an essential transcription factor during embryogenesis in Drosophila.. Genes Dev. 5: 1672-1683 [Abstract]  
• Roberts, S., Segil, N, Heintz, N (1991). Differential phosphorylation of the transcription factor Oct1 during the cell cycle. Science 253: 1022-1026 [Abstract]  
• Weintraub, H, Dwarki, V J, Verma, I, Davis, R, Hollenberg, S, Snider, L, Lassar, A, Tapscott, S J (1991). Muscle-specific transcriptional activation by MyoD.. Genes Dev. 5: 1377-1386 [Abstract]