This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, E A
Right arrow Articles by Hanson, R W
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, E A
Right arrow Articles by Hanson, R W

 Previous Article  |  Next Article 

Mol Cell Biol. 1990 December; 10(12): 6264-6272

The role of the CCAAT/enhancer-binding protein in the transcriptional regulation of the gene for phosphoenolpyruvate carboxykinase (GTP).

E A Park, W J Roesler, J Liu, D J Klemm, A L Gurney, J D Thatcher, J Shuman, A Friedman and R W Hanson

Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.

ABSTRACT

Previous studies have identified a region in the promoter of the gene for phosphoenolpyruvate carboxykinase (GTP) (PEPCK) (positions -460 to +73) containing the regulatory elements which respond to cyclic AMP, glucocorticoids, and insulin and confer the tissue- and developmental stage-specific properties to the gene. We report that CCAAT/enhancer-binding protein (C/EBP) binds to the cyclic AMP-responsive element CRE-1 as well as to two regions which have been previously shown to bind proteins enriched in liver nuclei. The DNase I footprint pattern provided by the recombinant C/EBP was identical to that produced by a 43-kDa protein purified from rat liver nuclear extracts, using a CRE oligonucleotide affinity column, which was originally thought to be the CRE-binding protein CREB. Transient contransfection experiments using a C/EBP expression vector demonstrated that C/EBP could trans activate the PEPCK promoter. The trans activation occurred through both the upstream, liver-specific protein-binding domains and the CRE. The CRE-binding protein bound only to CRE-1 and not to the upstream C/EBP-binding sites. The results of this study, along with physiological properties of C/EBP, indicate an important role for this transcription factor in providing the PEPCK gene with several of its regulatory characteristics.

Mol Cell Biol. 1990 December; 10(12): 6264-6272




This article has been cited by other articles:

  • Banzet, S., Koulmann, N., Simler, N., Sanchez, H., Chapot, R., Serrurier, B., Peinnequin, A., Bigard, X. (2009). Control of gluconeogenic genes during intense/prolonged exercise: hormone-independent effect of muscle-derived IL-6 on hepatic tissue and PEPCK mRNA. J. Appl. Physiol. 107: 1830-1839 [Abstract] [Full Text]  
  • Tomaru, T., Steger, D. J., Lefterova, M. I., Schupp, M., Lazar, M. A. (2009). Adipocyte-specific Expression of Murine Resistin Is Mediated by Synergism between Peroxisome Proliferator-activated Receptor {gamma} and CCAAT/Enhancer-binding Proteins. J. Biol. Chem. 284: 6116-6125 [Abstract] [Full Text]  
  • Wang, H., Larris, B., Peiris, T. H., Zhang, L., Le Lay, J., Gao, Y., Greenbaum, L. E. (2007). C/EBPbeta Activates E2F-regulated Genes in Vivo via Recruitment of the Coactivator CREB-binding Protein/P300. J. Biol. Chem. 282: 24679-24688 [Abstract] [Full Text]  
  • Onuma, H., Vander Kooi, B. T., Boustead, J. N., Oeser, J. K., O'Brien, R. M. (2006). Correlation between FOXO1a (FKHR) and FOXO3a (FKHRL1) Binding and the Inhibition of Basal Glucose-6-Phosphatase Catalytic Subunit Gene Transcription by Insulin. Mol. Endocrinol. 20: 2831-2847 [Abstract] [Full Text]  
  • McFie, P. J., Wang, G.-L., Timchenko, N. A., Wilson, H. L., Hu, X., Roesler, W. J. (2006). Identification of a Co-repressor That Inhibits the Transcriptional and Growth-Arrest Activities of CCAAT/Enhancer-binding Protein {alpha}. J. Biol. Chem. 281: 18069-18080 [Abstract] [Full Text]  
  • Herzog, B., Cardenas, J., Hall, R. K., Villena, J. A., Budge, P. J., Giguere, V., Granner, D. K., Kralli, A. (2006). Estrogen-related Receptor {alpha} Is a Repressor of Phosphoenolpyruvate Carboxykinase Gene Transcription. J. Biol. Chem. 281: 99-106 [Abstract] [Full Text]  
  • Yang, J., Croniger, C. M., Lekstrom-Himes, J., Zhang, P., Fenyus, M., Tenen, D. G., Darlington, G. J., Hanson, R. W. (2005). Metabolic Response of Mice to a Postnatal Ablation of CCAAT/Enhancer-binding Protein {alpha}. J. Biol. Chem. 280: 38689-38699 [Abstract] [Full Text]  
  • Yeagley, D., Quinn, P. G. (2005). 3',5'-Cyclic Adenosine Monophosphate Response Element-Binding Protein and CCAAT Enhancer-Binding Protein Are Dispensable for Insulin Inhibition of Phosphoenolpyruvate Carboxykinase Transcription and for Its Synergistic Induction by Protein Kinase A and Glucocorticoids. Mol. Endocrinol. 19: 913-924 [Abstract] [Full Text]  
  • Shao, J., Qiao, L., Janssen, R. C., Pagliassotti, M., Friedman, J. E. (2005). Chronic Hyperglycemia Enhances PEPCK Gene Expression and Hepatocellular Glucose Production Via Elevated Liver Activating Protein/Liver Inhibitory Protein Ratio. Diabetes 54: 976-984 [Abstract] [Full Text]  
  • Hanson, R. W. (2005). Metabolism in the Era of Molecular Biology. J. Biol. Chem. 280: 1705-1715 [Full Text]  
  • Olswang, Y., Blum, B., Cassuto, H., Cohen, H., Biberman, Y., Hanson, R. W., Reshef, L. (2003). Glucocorticoids Repress Transcription of Phosphoenolpyruvate Carboxykinase (GTP) Gene in Adipocytes by Inhibiting Its C/EBP-mediated Activation. J. Biol. Chem. 278: 12929-12936 [Abstract] [Full Text]  
  • Subramanian, L., Benson, M. D., Iniguez-Lluhi, J. A. (2003). A Synergy Control Motif within the Attenuator Domain of CCAAT/Enhancer-binding Protein alpha Inhibits Transcriptional Synergy through Its PIASy-enhanced Modification by SUMO-1 or SUMO-3. J. Biol. Chem. 278: 9134-9141 [Abstract] [Full Text]  
  • Young Lee, M., Jung, C.-H., Lee, K., Hyun Choi, Y., Hong, S., Cheong, J. (2002). Activating Transcription Factor-2 Mediates Transcriptional Regulation of Gluconeogenic Gene PEPCK by Retinoic Acid. Diabetes 51: 3400-3407 [Abstract] [Full Text]  
  • Melzner, I., Scott, V., Dorsch, K., Fischer, P., Wabitsch, M., Bruderlein, S., Hasel, C., Moller, P. (2002). Leptin Gene Expression in Human Preadipocytes Is Switched on by Maturation-induced Demethylation of Distinct CpGs in Its Proximal Promoter. J. Biol. Chem. 277: 45420-45427 [Abstract] [Full Text]  
  • Wilson, H. L., McFie, P. J., Roesler, W. J. (2002). Different Transcription Factor Binding Arrays Modulate the cAMP Responsivity of the Phosphoenolpyruvate Carboxykinase Gene Promoter. J. Biol. Chem. 277: 43895-43902 [Abstract] [Full Text]  
  • Carmona, M. C., Iglesias, R., Obregon, M.-J., Darlington, G. J., Villarroya, F., Giralt, M. (2002). Mitochondrial Biogenesis and Thyroid Status Maturation in Brown Fat Require CCAAT/Enhancer-binding Protein alpha. J. Biol. Chem. 277: 21489-21498 [Abstract] [Full Text]  
  • Gombart, A. F., Hofmann, W.-K., Kawano, S., Takeuchi, S., Krug, U., Kwok, S. H., Larsen, R. J., Asou, H., Miller, C. W., Hoelzer, D., Koeffler, H. P. (2002). Mutations in the gene encoding the transcription factor CCAAT/enhancer binding protein alpha in myelodysplastic syndromes and acute myeloid leukemias. Blood 99: 1332-1340 [Abstract] [Full Text]  
  • Liu, X., Wall, Q.-T., Taylor, L., Curthoys, N. P. (2001). C/EBPbeta contributes to cAMP-activated transcription of phosphoenolpyruvate carboxykinase in LLC-PK1-F+ cells. Am. J. Physiol. Renal Physiol. 281: F649-F657 [Abstract] [Full Text]  
  • Fasshauer, M., Klein, J., Kriauciunas, K. M., Ueki, K., Benito, M., Kahn, C. R. (2001). Essential Role of Insulin Receptor Substrate 1 in Differentiation of Brown Adipocytes. Mol. Cell. Biol. 21: 319-329 [Abstract] [Full Text]  
  • Calkhoven, C. F., Müller, C., Leutz, A. (2000). Translational control of C/EBPalpha and C/EBPbeta isoform expression. Genes Dev. 14: 1920-1932 [Abstract] [Full Text]  
  • Rosen, E. D., Walkey, C. J., Puigserver, P., Spiegelman, B. M. (2000). Transcriptional regulation of adipogenesis. Genes Dev. 14: 1293-1307 [Full Text]  
  • Crosson, S. M., Roesler, W. J. (2000). Hormonal Regulation of the Phosphoenolpyruvate Carboxykinase Gene. ROLE OF SPECIFIC CCAAT/ENHANCER-BINDING PROTEIN ISOFORMS. J. Biol. Chem. 275: 5804-5809 [Abstract] [Full Text]  
  • Jacob, K. K., Stanley, F. M. (1999). CCAAT/Enhancer-Binding Protein {alpha} Is a Physiological Regulator of Prolactin Gene Expression. Endocrinology 140: 4542-4550 [Abstract] [Full Text]  
  • Arizmendi, C., Liu, S., Croniger, C., Poli, V., Friedman, J. E. (1999). The Transcription Factor CCAAT/Enhancer-binding Protein beta  Regulates Gluconeogenesis and Phosphoenolpyruvate Carboxykinase (GTP) Gene Transcription during Diabetes. J. Biol. Chem. 274: 13033-13040 [Abstract] [Full Text]  
  • Leahy, P., Crawford, D. R., Grossman, G., Gronostajski, R. M., Hanson, R. W. (1999). CREB Binding Protein Coordinates the Function of Multiple Transcription Factors Including Nuclear Factor I to Regulate Phosphoenolpyruvate Carboxykinase (GTP) Gene Transcription. J. Biol. Chem. 274: 8813-8822 [Abstract] [Full Text]  
  • Yamada, K., Duong, D. T., Scott, D. K., Wang, J.-C., Granner, D. K. (1999). CCAAT/Enhancer-binding Protein beta  Is an Accessory Factor for the Glucocorticoid Response from the cAMP Response Element in the Rat Phosphoenolpyruvate Carboxykinase Gene Promoter. J. Biol. Chem. 274: 5880-5887 [Abstract] [Full Text]  
  • Park, E. A., Song, S., Vinson, C., Roesler, W. J. (1999). Role of CCAAT Enhancer-binding Protein beta  in the Thyroid Hormone and cAMP Induction of Phosphoenolpyruvate Carboxykinase Gene Transcription. J. Biol. Chem. 274: 211-217 [Abstract] [Full Text]  
  • Buggs, C., Nasrin, N., Mode, A., Tollet, P., Zhao, H.-F., Gustafsson, J.-A., Alexander-Bridges, M. (1998). IRE-ABP (Insulin Response Element-A Binding Protein), an SRY-Like Protein, Inhibits C/EBP{alpha} (CCAAT/Enhancer-Binding Protein {alpha})-Stimulated Expression of the Sex-Specific Cytochrome P450 2C12 Gene. Mol. Endocrinol. 12: 1294-1309 [Abstract] [Full Text]  
  • Cheong, J., Coligan, J. E., Shuman, J. D. (1998). Activating Transcription Factor-2 Regulates Phosphoenolpyruvate Carboxykinase Transcription through a Stress-inducible Mitogen-activated Protein Kinase Pathway. J. Biol. Chem. 273: 22714-22718 [Abstract] [Full Text]  
  • Yeagley, D., Agati, J. M., Quinn, P. G. (1998). A Tripartite Array of Transcription Factor Binding Sites Mediates cAMP Induction of Phosphoenolpyruvate Carboxykinase Gene Transcription and Its Inhibition by Insulin. J. Biol. Chem. 273: 18743-18750 [Abstract] [Full Text]  
  • Agati, J. M., Yeagley, D., Quinn, P. G. (1998). Assessment of the Roles of Mitogen-activated Protein Kinase, Phosphatidylinositol 3-Kinase, Protein Kinase B, and Protein Kinase C in Insulin Inhibition of cAMP-induced Phosphoenolpyruvate Carboxykinase Gene Transcription. J. Biol. Chem. 273: 18751-18759 [Abstract] [Full Text]  
  • Yubero, P., Barberá, M., Alvarez, R., Viñas, O., Mampel, T., Iglesias, R., Villarroya, F., Giralt, M. (1998). Dominant Negative Regulation by c-Jun of Transcription of the Uncoupling Protein-1 Gene through a Proximal cAMP-Regulatory Element: A Mechanism for Repressing Basal and Norepinephrine-Induced Expression of the Gene before Brown Adipocyte Differentiation. Mol. Endocrinol. 12: 1023-1037 [Abstract] [Full Text]  
  • Roesler, W. J., Park, E. A., McFie, P. J. (1998). Characterization of CCAAT/Enhancer-binding Protein alpha  as a Cyclic AMP-responsive Nuclear Regulator. J. Biol. Chem. 273: 14950-14957 [Abstract] [Full Text]  
  • Ono, F., Nakagawa, T., Saito, S., Owada, Y., Sakagami, H., Goto, K., Suzuki, M., Matsuno, S., Kondo, H. (1998). A Novel Class II Phosphoinositide 3-Kinase Predominantly Expressed in the Liver and Its Enhanced Expression during Liver Regeneration. J. Biol. Chem. 273: 7731-7736 [Abstract] [Full Text]  
  • Abbott, D. W., Holt, J. T. (1997). Finkel-Biskis-Reilly Osteosarcoma Virus v-Fos Inhibits Adipogenesis and Both the Activity and Expression of CCAAT/Enhancer Binding Protein alpha , a Key Regulator of Adipocyte Differentiation. J. Biol. Chem. 272: 32454-32462 [Abstract] [Full Text]  
  • Croniger, C., Trus, M., Lysek-Stupp, K., Cohen, H., Liu, Y., Darlington, G. J., Poli, V., Hanson, R. W., Reshef, L. (1997). Role of the Isoforms of CCAAT/Enhancer-binding Protein in the Initiation of Phosphoenolpyruvate Carboxykinase (GTP) Gene Transcription at Birth. J. Biol. Chem. 272: 26306-26312 [Abstract] [Full Text]  
  • Bahouth, S. W., Cui, X., Beauchamp, M. J., Shimomura, H., George, S. T., Park, E. A. (1997). Promoter Analysis of the Rat beta 1-Adrenergic Receptor Gene Identifies Sequences Involved in Basal Expression. Mol. Pharmacol. 51: 620-629 [Abstract] [Full Text]  
  • Hollenberg, A. N., Susulic, V. S., Madura, J. P., Zhang, B., Moller, D. E., Tontonoz, P., Sarraf, P., Spiegelman, B. M., Lowell, B. B. (1997). Functional Antagonism between CCAAT/Enhancer Binding Protein-alpha and Peroxisome Proliferator-activated Receptor-gamma on the Leptin Promoter. J. Biol. Chem. 272: 5283-5290 [Abstract] [Full Text]  
  • Savon, S. P., Hakimi, P., Crawford, D. R., Klemm, D. J., Gurney, A. L., Hanson, R. W. (1997). The Promoter Regulatory Regions of the Genes for the Cytosolic Form of Phosphoenolpyruvate Carboxykinase (GTP) from the Chicken and the Rat Have Different Species-Specific Roles in Gluconeogenesis. J. Nutr. 127: 276-285 [Abstract] [Full Text]  
  • Flodby, P., Barlow, C., Kylefjord, H., Ahrlund-Richter, L., Xanthopoulos, K. G. (1996). Increased Hepatic Cell Proliferation and Lung Abnormalities in Mice Deficient in CCAAT/Enhancer Binding Protein alpha. J. Biol. Chem. 271: 24753-24760 [Abstract] [Full Text]  
  • Kim, J B, Spiegelman, B M (1996). ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism.. Genes Dev. 10: 1096-1107 [Abstract]  
  • Brun, R P, Tontonoz, P, Forman, B M, Ellis, R, Chen, J, Evans, R M, Spiegelman, B M (1996). Differential activation of adipogenesis by multiple PPAR isoforms.. Genes Dev. 10: 974-984 [Abstract]  
  • Roesler, W. J., Crosson, S. M., Vinson, C., McFie, P. J. (1996). The alpha-Isoform of the CCAAT/Enhancer-binding Protein Is Required for Mediating cAMP Responsiveness of the Phosphoenolpyruvate Carboxykinase Promoter in Hepatoma Cells. J. Biol. Chem. 271: 8068-8074 [Abstract] [Full Text]  
  • Klemm, D. J., Colton, L. A., Ryan, S., Routes, J. M. (1996). Adenovirus E1A Proteins Regulate Phosphoenolpyruvate Carboxykinase Gene Transcription through Multiple Mechanisms. J. Biol. Chem. 271: 8082-8088 [Abstract] [Full Text]  
  • Wu, Z, Xie, Y, Bucher, N L, Farmer, S R (1995). Conditional ectopic expression of C/EBP beta in NIH-3T3 cells induces PPAR gamma and stimulates adipogenesis.. Genes Dev. 9: 2350-2363 [Abstract]  
  • Wang, N., Finegold, M., Bradley, A, Ou, C., Abdelsayed, S., Wilde, M., Taylor, L., Wilson, D., Darlington, G. (1995). Impaired energy homeostasis in C/EBP alpha knockout mice. Science 269: 1108-1112 [Abstract]  
  • Goraya, T. Y., Kessler, S. P., Stanton, P., Hanson, R. W., Sen, G. C. (1995). The Cyclic AMP Response Elements of the Genes for Angiotensin-converting Enzyme and Phosphoenolpyruvate Carboxykinase (GTP) Can Mediate Transcriptional Activation by CREM[IMAGE] and CREMalpha. J. Biol. Chem. 270: 19078-19085 [Abstract] [Full Text]  
  • Rana, B., Xie, Y., Mischoulon, D., Bucher, N. L. R., Farmer, S. R. (1995). The DNA Binding Activity of C/EBP Transcription Factors Is Regulated in the G[IMAGE] Phase of the Hepatocyte Cell Cycle. J. Biol. Chem. 270: 18123-18132 [Abstract] [Full Text]  
  • Roesler, W. J., Graham, J. G., Kolen, R., Klemm, D. J., McFie, P. J. (1995). The cAMP Response Element Binding Protein Synergizes with Other Transcription Factors to Mediate cAMP Responsiveness. J. Biol. Chem. 270: 8225-8232 [Abstract] [Full Text]  
  • Freytag, S O, Paielli, D L, Gilbert, J D (1994). Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the adipogenic program in a variety of mouse fibroblastic cells.. Genes Dev. 8: 1654-1663 [Abstract]  
  • Angrand, P. O., Rousset, J. P., Weiss, M. C. (1992). Cell phenotype, binding affinity and promoter structure modulate transactivation by HNF1 and LAP. J. Cell Sci. 103: 1083-1092 [Abstract]  
  • Ron, D, Habener, J F (1992). CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription.. Genes Dev. 6: 439-453 [Abstract]  
  • Metz, R, Ziff, E (1991). cAMP stimulates the C/EBP-related transcription factor rNFIL-6 to trans-locate to the nucleus and induce c-fos transcription.. Genes Dev. 5: 1754-1766 [Abstract]  
  • Cao, Z, Umek, R M, McKnight, S L (1991). Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells.. Genes Dev. 5: 1538-1552 [Abstract]  
  • Wang, L., Shao, J., Muhlenkamp, P., Liu, S., Klepcyk, P., Ren, J., Friedman, J. E. (2000). Increased Insulin Receptor Substrate-1 and Enhanced Skeletal Muscle Insulin Sensitivity in Mice Lacking CCAAT/Enhancer-binding Protein beta. J. Biol. Chem. 275: 14173-14181 [Abstract] [Full Text]  
  • Guo, S., Cichy, S. B., He, X., Yang, Q., Ragland, M., Ghosh, A. K., Johnson, P. F., Unterman, T. G. (2001). Insulin Suppresses Transactivation by CAAT/Enhancer-binding Proteins beta (C/EBPbeta ). SIGNALING TO p300/CREB-BINDING PROTEIN BY PROTEIN KINASE B DISRUPTS INTERACTION WITH THE MAJOR ACTIVATION DOMAIN OF C/EBPbeta. J. Biol. Chem. 276: 8516-8523 [Abstract] [Full Text]  
  • Prosch, S., Heine, A.-K., Volk, H.-D., Kruger, D. H. (2001). CCAAT/Enhancer-binding Proteins alpha and beta Negatively Influence the Capacity of Tumor Necrosis Factor alpha to Up-regulate the Human Cytomegalovirus IE1/2 Enhancer/Promoter by Nuclear Factor kappa B during Monocyte Differentiation. J. Biol. Chem. 276: 40712-40720 [Abstract] [Full Text]  
  • Yeagley, D., Moll, J., Vinson, C. A., Quinn, P. G. (2000). Characterization of Elements Mediating Regulation of Phosphoenolpyruvate Carboxykinase Gene Transcription by Protein Kinase A and Insulin. IDENTIFICATION OF A DISTINCT COMPLEX FORMED IN CELLS THAT MEDIATE INSULIN INHIBITION. J. Biol. Chem. 275: 17814-17820 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»