This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aplan, P D
Right arrow Articles by Kirsch, I R
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aplan, P D
Right arrow Articles by Kirsch, I R

 Previous Article  |  Next Article 

Mol Cell Biol. 1990 December; 10(12): 6426-6435

The SCL gene is formed from a transcriptionally complex locus.

P D Aplan, C G Begley, V Bertness, M Nussmeier, A Ezquerra, J Coligan and I R Kirsch

Navy Medical Branch, National Cancer Institute, Bethesda, Maryland 20814.

ABSTRACT

We describe the structural organization of the human SCL gene, a helix-loop-helix family member which we believe plays a fundamental role in hematopoietic differentiation. The SCL locus is composed of eight exons distributed over 16 kb. SCL shows a pattern of expression quite restricted to early hematopoietic tissues, although in malignant states expression of the gene may be somewhat extended into later developmental stages. A detailed analysis of the transcript(s) arising from the SCL locus revealed that (i) the 5' noncoding portion of the SCL transcript, which resides within a CpG island, has a complex pattern of alternative exon utilization as well as two distinct transcription initiation sites; (ii) the 5' portions of the SCL transcript contain features that suggest a possible regulatory role for these segments; (iii) the pattern of utilization of the 5' exons is cell lineage dependent; and (iv) all of the currently studied chromosomal aberrations that affect the SCL locus either structurally or functionally eliminate the normal 5' transcription initiation sites. These data suggest that the SCL gene, and specifically its 5' region, may be a target for regulatory interactions during early hematopoietic development.

Mol Cell Biol. 1990 December; 10(12): 6426-6435




This article has been cited by other articles:

  • Bockamp, E., Antunes, C., Maringer, M., Heck, R., Presser, K., Beilke, S., Ohngemach, S., Alt, R., Cross, M., Sprengel, R., Hartwig, U., Kaina, B., Schmitt, S., Eshkind, L. (2006). Tetracycline-controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes, and c-kit-expressing lineage-negative hematopoietic cells. Blood 108: 1533-1541 [Abstract] [Full Text]  
  • Lazrak, M., Deleuze, V., Noel, D., Haouzi, D., Chalhoub, E., Dohet, C., Robbins, I., Mathieu, D. (2004). The bHLH TAL-1/SCL regulates endothelial cell migration and morphogenesis. J. Cell Sci. 117: 1161-1171 [Abstract] [Full Text]  
  • Calkhoven, C. F., Muller, C., Martin, R., Krosl, G., Hoang, T., Leutz, A. (2003). Translational control of SCL-isoform expression in hematopoietic lineage choice. Genes Dev. 17: 959-964 [Abstract] [Full Text]  
  • Tang, T., Arbiser, J. L., Brandt, S. J. (2002). Phosphorylation by Mitogen-activated Protein Kinase Mediates the Hypoxia-induced Turnover of the TAL1/SCL Transcription Factor in Endothelial Cells. J. Biol. Chem. 277: 18365-18372 [Abstract] [Full Text]  
  • Sinclair, A. M., Bench, A. J., Bloor, A. J. C., Li, J., Gottgens, B., Stanley, M. L., Miller, J., Piltz, S., Hunter, S., Nacheva, E. P., Sanchez, M.-J., Green, A. R. (2002). Rescue of the lethal scl-/- phenotype by the human SCL locus. Blood 99: 3931-3938 [Abstract] [Full Text]  
  • Mead, P. E., Deconinck, A. E., Huber, T. L., Orkin, S. H., Zon, L. I. (2001). Primitive erythropoiesis in the Xenopus embryo: the synergistic role of LMO-2, SCL and GATA-binding proteins. Development 128: 2301-2308 [Abstract] [Full Text]  
  • Courtes, C., Lecointe, N., Le Cam, L., Baudoin, F., Sardet, C., Mathieu-Mahul, D. (2000). Erythroid-specific Inhibition of the tal-1 Intragenic Promoter Is Due to Binding of a Repressor to a Novel Silencer. J. Biol. Chem. 275: 949-958 [Abstract] [Full Text]  
  • Chervinsky, D. S., Zhao, X.-F., Lam, D. H., Ellsworth, M., Gross, K. W., Aplan, P. D. (1999). Disordered T-Cell Development and T-Cell Malignancies in SCL LMO1 Double-Transgenic Mice: Parallels with E2A-Deficient Mice. Mol. Cell. Biol. 19: 5025-5035 [Abstract] [Full Text]  
  • Begley, C. G., Green, A. R. (1999). The SCL Gene: From Case Report to Critical Hematopoietic Regulator. Blood 93: 2760-2770 [Full Text]  
  • Zhao, X.-F., Aplan, P. D. (1999). The Hematopoietic Transcription Factor SCL Binds the p44 Subunit of TFIIH. J. Biol. Chem. 274: 1388-1393 [Abstract] [Full Text]  
  • Sanchez, M, Gottgens, B, Sinclair, A., Stanley, M, Begley, C., Hunter, S, Green, A. (1999). An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors. Development 126: 3891-3904 [Abstract]  
  • Minegishi, N., Ohta, J., Suwabe, N., Nakauchi, H., Ishihara, H., Hayashi, N., Yamamoto, M. (1998). Alternative Promoters Regulate Transcription of the Mouse GATA-2 Gene. J. Biol. Chem. 273: 3625-3634 [Abstract] [Full Text]  
  • Goldfarb, A. N., Lewandowska, K., Pennell, C. A. (1998). Identification of a Highly Conserved Module in E Proteins Required for in Vivo Helix-loop-helix Dimerization. J. Biol. Chem. 273: 2866-2873 [Abstract] [Full Text]  
  • Mead, P., Kelley, C., Hahn, P., Piedad, O, Zon, L. (1998). SCL specifies hematopoietic mesoderm in Xenopus embryos. Development 125: 2611-2620 [Abstract]  
  • Zhang, M.-Y., Clawson, G. A., Olivieri, N. F., Bell, L. L., Begley, C. G., Miller, B. A. (1997). Expression of SCL Is Normal in Transfusion-Dependent Diamond-Blackfan Anemia But Other bHLH Proteins Are Deficient. Blood 90: 2068-2074 [Abstract] [Full Text]  
  • Bockamp, E.-O., McLaughlin, F., Gottgens, B., Murrell, A. M., Elefanty, A. G., Green, A. R. (1997). Distinct Mechanisms Direct SCL/tal-1 Expression in Erythroid Cells and CD34 Positive Primitive Myeloid Cells. J. Biol. Chem. 272: 8781-8790 [Abstract] [Full Text]  
  • Wu, Y.-j., Fraizer, G. C., Saunders, G. F. (1995). GATA-1 Transactivates the WT1 Hematopoietic Specific Enhancer. J. Biol. Chem. 270: 5944-5949 [Abstract] [Full Text]  
  • Barton, L. M., Gottgens, B., Gering, M., Gilbert, J. G. R., Grafham, D., Rogers, J., Bentley, D., Patient, R., Green, A. R. (2001). From the Cover: Regulation of the stem cell leukemia (SCL) gene: A tale of two fishes. Proc. Natl. Acad. Sci. USA 98: 6747-6752 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»