This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herman, P K
Right arrow Articles by Emr, S D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herman, P K
Right arrow Articles by Emr, S D

 Previous Article  |  Next Article 

Mol Cell Biol. 1990 December; 10(12): 6742-6754

Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae.

P K Herman and S D Emr

Division of Biology, California Institute of Technology, Pasadena 91125.

ABSTRACT

VPS34 gene function is required for the efficient localization of a variety of vacuolar proteins. We have cloned and sequenced the wild-type VPS34 gene in order to gain a better understanding of the role of its protein product in this intracellular sorting pathway. Interestingly, disruption of the VPS34 locus resulted in a temperature-sensitive growth defect, indicating that the VPS34 gene is essential for vegetative growth only at elevated growth temperatures. As with the original vps34 alleles, vps34 null mutants exhibited severe vacuolar protein sorting defects and possessed a morphologically normal vacuolar structure. The VPS34 gene DNA sequence identifies an open reading frame that could encode a hydrophilic protein of 875 amino acids. The predicted protein sequence lacks any apparent signal sequence or membrane-spanning domains, suggesting that Vps34p does not enter the secretory pathway. Results from immunoprecipitation experiments with antiserum prepared against a TrpE-Vps34 fusion protein were consistent with this prediction: a rare, unglycosylated protein of approximately 95,000 Da was detected in extracts of wild-type Saccharomyces cerevisiae cells. Cell fractionation studies indicated that a significant portion of the Vps34p is found associated with a particulate fraction of yeast cells. This particulate Vps34p was readily solubilized by treatment with 2 M urea but not with Triton X-100, suggesting that the presence of Vps34p in this pelletable structure is mediated by protein-protein interactions. vp34 mutant cells also exhibited a defect in the normal partitioning of the vacuolar compartment between mother and daughter cells during cell division. In more than 80% of the delta vps34 dividing cells examined, no vacuolar structures were observed in the newly emerging bud, whereas in wild-type dividing cells, more than 95% of the buds had a detectable vacuolar compartment. Our results suggest that the Vps34p may act as a component of a relatively large intracellular structure that functions to facilitate specific steps of the vacuolar protein delivery and inheritance pathways.

Mol Cell Biol. 1990 December; 10(12): 6742-6754




This article has been cited by other articles:

  • MacKenzie, M. G., Hamilton, D. L., Murray, J. T., Taylor, P. M., Baar, K. (2009). mVps34 is activated following high-resistance contractions. J. Physiol. 587: 253-260 [Abstract] [Full Text]  
  • Dehring, D. A. K., Adler, A. S., Hosseini, A., Hicke, L. (2008). A C-terminal Sequence in the Guanine Nucleotide Exchange Factor Sec7 Mediates Golgi Association and Interaction with the Rsp5 Ubiquitin Ligase. J. Biol. Chem. 283: 34188-34196 [Abstract] [Full Text]  
  • Kumar, G. R., Goyashiki, R., Ramakrishnan, V., Karpel, J. E., Bisson, L. F. (2008). Genes Required for Ethanol Tolerance and Utilization in Saccharomyces cerevisiae. Am. J. Enol. Vitic. 59: 401-411 [Abstract] [Full Text]  
  • Hall, B. S., Gabernet-Castello, C., Voak, A., Goulding, D., Natesan, S. K., Field, M. C. (2006). TbVps34, the Trypanosome Orthologue of Vps34, Is Required for Golgi Complex Segregation. J. Biol. Chem. 281: 27600-27612 [Abstract] [Full Text]  
  • Obara, K., Sekito, T., Ohsumi, Y. (2006). Assortment of Phosphatidylinositol 3-Kinase Complexes--Atg14p Directs Association of Complex I to the Pre-autophagosomal Structure in Saccharomyces cerevisiae. Mol. Biol. Cell 17: 1527-1539 [Abstract] [Full Text]  
  • Parrish, W. R., Stefan, C. J., Emr, S. D. (2005). PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathway. J. Cell Sci. 118: 5589-5601 [Abstract] [Full Text]  
  • Byfield, M. P., Murray, J. T., Backer, J. M. (2005). hVps34 Is a Nutrient-regulated Lipid Kinase Required for Activation of p70 S6 Kinase. J. Biol. Chem. 280: 33076-33082 [Abstract] [Full Text]  
  • Gunther, J., Nguyen, M., Hartl, A., Kunkel, W., Zipfel, P. F., Eck, R. (2005). Generation and functional in vivo characterization of a lipid kinase defective phosphatidylinositol 3-kinase Vps34p of Candida albicans. Microbiology 151: 81-89 [Abstract] [Full Text]  
  • Stromhaug, P. E., Reggiori, F., Guan, J., Wang, C.-W., Klionsky, D. J. (2004). Atg21 Is a Phosphoinositide Binding Protein Required for Efficient Lipidation and Localization of Atg8 during Uptake of Aminopeptidase I by Selective Autophagy. Mol. Biol. Cell 15: 3553-3566 [Abstract] [Full Text]  
  • Roth, M. G. (2004). Phosphoinositides in Constitutive Membrane Traffic. Physiol. Rev. 84: 699-730 [Abstract] [Full Text]  
  • MacDougall, L. K., Gagou, M. E., Leevers, S. J., Hafen, E., Waterfield, M. D. (2004). Targeted Expression of the Class II Phosphoinositide 3-Kinase in Drosophila melanogaster Reveals Lipid Kinase-Dependent Effects on Patterning and Interactions with Receptor Signaling Pathways. Mol. Cell. Biol. 24: 796-808 [Abstract] [Full Text]  
  • Mochizuki, Y., Majerus, P. W. (2003). Characterization of myotubularin-related protein 7 and its binding partner, myotubularin-related protein 9. Proc. Natl. Acad. Sci. USA 100: 9768-9773 [Abstract] [Full Text]  
  • TUMA, P. L., HUBBARD, A. L. (2003). Transcytosis: Crossing Cellular Barriers. Physiol. Rev. 83: 871-932 [Abstract] [Full Text]  
  • Uttamsingh, S., Zong, C. S., Wang, L.-H. (2003). Matrix-independent Activation of Phosphatidylinositol 3-Kinase, Stat3, and Cyclin A-associated Cdk2 Is Essential for Anchorage-independent Growth of v-Ros-transformed Chicken Embryo Fibroblasts. J. Biol. Chem. 278: 18798-18810 [Abstract] [Full Text]  
  • Han, G.-S., Audhya, A., Markley, D. J., Emr, S. D., Carman, G. M. (2002). The Saccharomyces cerevisiae LSB6 Gene Encodes Phosphatidylinositol 4-Kinase Activity. J. Biol. Chem. 277: 47709-47718 [Abstract] [Full Text]  
  • Burda, P., Padilla, S. M., Sarkar, S., Emr, S. D. (2002). Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase. J. Cell Sci. 115: 3889-3900 [Abstract] [Full Text]  
  • Mueller-Roeber, B., Pical, C. (2002). Inositol Phospholipid Metabolism in Arabidopsis. Characterized and Putative Isoforms of Inositol Phospholipid Kinase and Phosphoinositide-Specific Phospholipase C. Plant Physiol. 130: 22-46 [Abstract] [Full Text]  
  • Hayashi, S., Okada, T., Igarashi, N., Fujita, T., Jahangeer, S., Nakamura, S.-i. (2002). Identification and Characterization of RPK118, a Novel Sphingosine Kinase-1-binding Protein. J. Biol. Chem. 277: 33319-33324 [Abstract] [Full Text]  
  • Wurmser, A. E., Emr, S. D. (2002). Novel PtdIns(3)P-binding protein Etf1 functions as an effector of the Vps34 PtdIns 3-kinase in autophagy. JCB 158: 761-772 [Abstract] [Full Text]  
  • Augsten, M., Hubner, C., Nguyen, M., Kunkel, W., Hartl, A., Eck, R. (2002). Defective Hyphal Induction of a Candida albicans Phosphatidylinositol 3-Phosphate 5-Kinase Null Mutant on Solid Media Does Not Lead to Decreased Virulence. Infect. Immun. 70: 4462-4470 [Abstract] [Full Text]  
  • Seaman, M. N.J., Williams, H. P. (2002). Identification of the Functional Domains of Yeast Sorting Nexins Vps5p and Vps17p. Mol. Biol. Cell 13: 2826-2840 [Abstract] [Full Text]  
  • Ellson, C. D., Andrews, S., Stephens, L. R., Hawkins, P. T. (2002). The PX domain: a new phosphoinositide-binding module. J. Cell Sci. 115: 1099-1105 [Abstract] [Full Text]  
  • Budovskaya, Y. V., Hama, H., DeWald, D. B., Herman, P. K. (2002). The C Terminus of the Vps34p Phosphoinositide 3-Kinase Is Necessary and Sufficient for the Interaction with the Vps15p Protein Kinase. J. Biol. Chem. 277: 287-294 [Abstract] [Full Text]  
  • Kim, J., Huang, W.-P., Stromhaug, P. E., Klionsky, D. J. (2002). Convergence of Multiple Autophagy and Cytoplasm to Vacuole Targeting Components to a Perivacuolar Membrane Compartment Prior to de Novo Vesicle Formation. J. Biol. Chem. 277: 763-773 [Abstract] [Full Text]  
  • Tuma, P. L., Nyasae, L. K., Backer, J. M., Hubbard, A. L. (2001). Vps34p differentially regulates endocytosis from the apical and basolateral domains in polarized hepatic cells. JCB 154: 1197-1208 [Abstract] [Full Text]  
  • Abeliovich, H., Klionsky, D. J. (2001). Autophagy in Yeast: Mechanistic Insights and Physiological Function. Microbiol. Mol. Biol. Rev. 65: 463-479 [Abstract] [Full Text]  
  • Kihara, A., Noda, T., Ishihara, N., Ohsumi, Y. (2001). Two Distinct Vps34 Phosphatidylinositol 3-Kinase Complexes Function in Autophagy and Carboxypeptidase Y Sorting inSaccharomyces cerevisiae. JCB 152: 519-530 [Abstract] [Full Text]  
  • Deloche, O., Yeung, B. G., Payne, G. S., Schekman, R. (2001). Vps10p Transport from the trans-Golgi Network to the Endosome Is Mediated by Clathrin-coated Vesicles. Mol. Biol. Cell 12: 475-485 [Abstract] [Full Text]  
  • Cremona, O, De Camilli, P (2001). Phosphoinositides in membrane traffic at the synapse. J. Cell Sci. 114: 1041-1052 [Abstract]  
  • Bruckmann, A., Künkel, W., Härtl, A., Wetzker, R., Eck, R. (2000). A phosphatidylinositol 3-kinase of Candida albicans influences adhesion, filamentous growth and virulence. Microbiology 146: 2755-2764 [Abstract] [Full Text]  
  • Arcaro, A., Zvelebil, M. J., Wallasch, C., Ullrich, A., Waterfield, M. D., Domin, J. (2000). Class II Phosphoinositide 3-Kinases Are Downstream Targets of Activated Polypeptide Growth Factor Receptors. Mol. Cell. Biol. 20: 3817-3830 [Abstract] [Full Text]  
  • Dell'Angelica, E. C., Puertollano, R., Mullins, C., Aguilar, R. C., Vargas, J. D., Hartnell, L. M., Bonifacino, J. S. (2000). Ggas: A Family of Adp Ribosylation Factor-Binding Proteins Related to Adaptors and Associated with the Golgi Complex. JCB 149: 81-94 [Abstract] [Full Text]  
  • Mayer, A., Scheglmann, D., Dove, S., Glatz, A., Wickner, W., Haas, A. (2000). Phosphatidylinositol 4,5-Bisphosphate Regulates Two Steps of Homotypic Vacuole Fusion. Mol. Biol. Cell 11: 807-817 [Abstract] [Full Text]  
  • Hughes, W. E., Woscholski, R., Cooke, F. T., Patrick, R. S., Dove, S. K., McDonald, N. Q., Parker, P. J. (2000). SAC1 Encodes a Regulated Lipid Phosphoinositide Phosphatase, Defects in Which Can Be Suppressed by the Homologous Inp52p and Inp53p Phosphatases. J. Biol. Chem. 275: 801-808 [Abstract] [Full Text]  
  • Chen, X., Van Valkenburgh, C., Fang, H., Green, N. (1999). Signal Peptides Having Standard and Nonstandard Cleavage Sites Can Be Processed by Imp1p of the Mitochondrial Inner Membrane Protease. J. Biol. Chem. 274: 37750-37754 [Abstract] [Full Text]  
  • McEwen, R. K., Dove, S. K., Cooke, F. T., Painter, G. F., Holmes, A. B., Shisheva, A., Ohya, Y., Parker, P. J., Michell, R. H. (1999). Complementation Analysis in PtdInsP Kinase-deficient Yeast Mutants Demonstrates That Schizosaccharomyces pombe and Murine Fab1p Homologues Are Phosphatidylinositol 3-Phosphate 5-Kinases. J. Biol. Chem. 274: 33905-33912 [Abstract] [Full Text]  
  • Hama, H., Schnieders, E. A., Thorner, J., Takemoto, J. Y., DeWald, D. B. (1999). Direct Involvement of Phosphatidylinositol 4-Phosphate in Secretion in the Yeast Saccharomyces cerevisiae. J. Biol. Chem. 274: 34294-34300 [Abstract] [Full Text]  
  • Edinger, R. S., Rokaw, M. D., Johnson, J. P. (1999). Vasopressin stimulates sodium transport in A6 cells via a phosphatidylinositide 3-kinase-dependent pathway. Am. J. Physiol. Renal Physiol. 277: F575-F579 [Abstract] [Full Text]  
  • Gillham, H., Golding, M. C.H.M., Pepperkok, R., Gullick, W. J. (1999). Intracellular Movement of Green Fluorescent Protein-Tagged Phosphatidylinositol 3-Kinase in Response to Growth Factor Receptor Signaling. JCB 146: 869-880 [Abstract] [Full Text]  
  • Vida, T., Gerhardt, B. (1999). A Cell-Free Assay Allows Reconstitution of Vps33p-Dependent Transport to the Yeast Vacuole/Lysosome. JCB 146: 85-98 [Abstract] [Full Text]  
  • Tall, G. G., Hama, H., DeWald, D. B., Horazdovsky, B. F. (1999). The Phosphatidylinositol 3-Phosphate Binding Protein Vac1p Interacts with a Rab GTPase and a Sec1p Homologue to Facilitate Vesicle-mediated Vacuolar Protein Sorting. Mol. Biol. Cell 10: 1873-1889 [Abstract] [Full Text]  
  • Tuma, P. L., Finnegan, C. M., Yi, J.-H., Hubbard, A. L. (1999). Evidence for Apical Endocytosis in Polarized Hepatic Cells: Phosphoinositide 3-Kinase Inhibitors Lead to the Lysosomal Accumulation of Resident Apical Plasma Membrane Proteins. JCB 145: 1089-1102 [Abstract] [Full Text]  
  • Stock, S. D., Hama, H., DeWald, D. B., Takemoto, J. Y. (1999). SEC14-dependent Secretion in Saccharomyces cerevisiae. NONDEPENDENCE ON SPHINGOLIPID SYNTHESIS-COUPLED DIACYLGLYCEROL PRODUCTION. J. Biol. Chem. 274: 12979-12983 [Abstract] [Full Text]  
  • Wurmser, A. E., Gary, J. D., Emr, S. D. (1999). Phosphoinositide 3-Kinases and Their FYVE Domain-containing Effectors as Regulators of Vacuolar/Lysosomal Membrane Trafficking Pathways. J. Biol. Chem. 274: 9129-9132 [Full Text]  
  • Siddhanta, U., McIlroy, J., Shah, A., Zhang, Y., Backer, J. M. (1998). Distinct Roles for the p110{alpha} and hVPS34 Phosphatidylinositol 3'-Kinases in Vesicular Trafficking, Regulation of the Actin Cytoskeleton, and Mitogenesis. JCB 143: 1647-1659 [Abstract] [Full Text]  
  • Saleh, A., Schieltz, D., Ting, N., McMahon, S. B., Litchfield, D. W., Yates III, J. R., Lees-Miller, S. P., Cole, M. D., Brandl, C. J. (1998). Tra1p Is a Component of the Yeast Ada·Spt Transcriptional Regulatory Complexes. J. Biol. Chem. 273: 26559-26565 [Abstract] [Full Text]  
  • Gary, J. D., Wurmser, A. E., Bonangelino, C. J., Weisman, L. S., Emr, S. D. (1998). Fab1p Is Essential for PtdIns(3)P 5-Kinase Activity and the Maintenance of Vacuolar Size and Membrane Homeostasis. JCB 143: 65-79 [Abstract] [Full Text]  
  • McCallum, S. J., Erickson, J. W., Cerione, R. A. (1998). Characterization of the Association of the Actin-binding Protein, IQGAP, and Activated Cdc42 with Golgi Membranes. J. Biol. Chem. 273: 22537-22544 [Abstract] [Full Text]  
  • Feranchak, A. P., Roman, R. M., Schwiebert, E. M., Fitz, J. G. (1998). Phosphatidylinositol 3-Kinase Contributes to Cell Volume Regulation through Effects on ATP Release. J. Biol. Chem. 273: 14906-14911 [Abstract] [Full Text]  
  • Stolz, L. E., Kuo, W. J., Longchamps, J., Sekhon, M. K., York, J. D. (1998). INP51, a Yeast Inositol Polyphosphate 5-Phosphatase Required for Phosphatidylinositol 4,5-Bisphosphate Homeostasis and Whose Absence Confers a Cold-resistant Phenotype. J. Biol. Chem. 273: 11852-11861 [Abstract] [Full Text]  
  • Liu, J., Fitzli, D., Liu, M., Tseu, I., Caniggia, I., Rotin, D., Post, M. (1998). PDGF-induced glycosaminoglycan synthesis is mediated via phosphatidylinositol 3-kinase. Am. J. Physiol. Lung Cell. Mol. Physiol. 274: L702-L713 [Abstract] [Full Text]  
  • Gaynor, E. C., Chen, C.-Y., Emr, S. D., Graham, T. R. (1998). ARF Is Required for Maintenance of Yeast Golgi and Endosome Structure and Function. Mol. Biol. Cell 9: 653-670 [Abstract] [Full Text]  
  • Fiorentino, D. F., Crabtree, G. R. (1997). Characterization of Saccharomyces cerevisiae dna2 Mutants Suggests a Role for the Helicase Late in S Phase. Mol. Biol. Cell 8: 2519-2537 [Abstract] [Full Text]  
  • Piper, R. C., Bryant, N. J., Stevens, T. H. (1997). The Membrane Protein Alkaline Phosphatase Is Delivered to the Vacuole by a Route That Is Distinct from the VPS-dependent Pathway. JCB 138: 531-545 [Abstract] [Full Text]  
  • Patki, V., Virbasius, J., Lane, W. S., Toh, B.-H., Shpetner, H. S., Corvera, S. (1997). Identification of an early endosomal protein regulated by phosphatidylinositol 3-kinase. Proc. Natl. Acad. Sci. USA 94: 7326-7330 [Abstract] [Full Text]  
  • Mukhopadhyay, A., Funato, K., Stahl, P. D. (1997). Rab7 Regulates Transport from Early to Late Endocytic Compartments in Xenopus Oocytes. J. Biol. Chem. 272: 13055-13059 [Abstract] [Full Text]  
  • Fang, H., Mullins, C., Green, N. (1997). In Addition to SEC11, a Newly Identified Gene, SPC3, Is Essential for Signal Peptidase Activity in the Yeast Endoplasmic Reticulum. J. Biol. Chem. 272: 13152-13158 [Abstract] [Full Text]  
  • Woscholski, R., Finan, P. M., Radley, E., Totty, N. F., Sterling, A. E., Hsuan, J. J., Waterfield, M. D., Parker, P. J. (1997). Synaptojanin Is the Major Constitutively Active Phosphatidylinositol-3,4,5-trisphosphate 5-Phosphatase in Rodent Brain. J. Biol. Chem. 272: 9625-9628 [Abstract] [Full Text]  
  • Klebanow, E. R., Poon, D., Zhou, S., Weil, P. A. (1997). Cloning and Characterization of an Essential Saccharomyces cerevisiae Gene, TAF40, Which Encodes yTAFII40, an RNA Polymerase II-specific TATA-binding Protein-associated Factor. J. Biol. Chem. 272: 9436-9442 [Abstract] [Full Text]  
  • Inukai, K., Funaki, M., Ogihara, T., Katagiri, H., Kanda, A., Anai, M., Fukushima, Y., Hosaka, T., Suzuki, M., Shin, B.-C., Takata, K., Yazaki, Y., Kikuchi, M., Oka, Y., Asano, T. (1997). p85alpha Gene Generates Three Isoforms of Regulatory Subunit for Phosphatidylinositol 3-Kinase (PI 3-Kinase), p50alpha , p55alpha , and p85alpha , with Different PI 3-Kinase Activity Elevating Responses to Insulin. J. Biol. Chem. 272: 7873-7882 [Abstract] [Full Text]  
  • Panaretou, C., Domin, J., Cockcroft, S., Waterfield, M. D. (1997). Characterization of p150, an Adaptor Protein for the Human Phosphatidylinositol (PtdIns) 3-Kinase. SUBSTRATE PRESENTATION BY PHOSPHATIDYLINOSITOL TRANSFER PROTEIN TO THE p150·PtdIns 3-KINASE COMPLEX. J. Biol. Chem. 272: 2477-2485 [Abstract] [Full Text]  
  • Horowitz, D S, Krainer, A R (1997). A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor.. Genes Dev. 11: 139-151 [Abstract]  
  • Horazdovsky, B. F., Cowles, C. R., Mustol, P., Holmes, M., Emr, S. D. (1996). A Novel RING Finger Protein, Vps8p, Functionally Interacts with the Small GTPase, Vps21p, to Facilitate Soluble Vacuolar Protein Localization. J. Biol. Chem. 271: 33607-33615 [Abstract] [Full Text]  
  • Mullins, C., Meyer, H.-A., Hartmann, E., Green, N., Fang, H. (1996). Structurally Related Spc1p and Spc2p of Yeast Signal Peptidase Complex Are Functionally Distinct. J. Biol. Chem. 271: 29094-29099 [Abstract] [Full Text]  
  • Uribe, J. M., Keely, S. J., Traynor-Kaplan, A. E., Barrett, K. E. (1996). Phosphatidylinositol 3-Kinase Mediates the Inhibitory Effect of Epidermal Growth Factor on Calcium-dependent Chloride Secretion. J. Biol. Chem. 271: 26588-26595 [Abstract] [Full Text]  
  • Fang, H., Panzner, S., Mullins, C., Hartmann, E., Green, N. (1996). The Homologue of Mammalian SPC12 Is Important for Efficient Signal Peptidase Activity in Saccharomyces cerevisiae. J. Biol. Chem. 271: 16460-16465 [Abstract] [Full Text]  
  • Carman, G. M., Zeimetz, G. M. (1996). Regulation of Phospholipid Biosynthesis in the Yeast Saccharomyces cerevisiae. J. Biol. Chem. 271: 13293-13296 [Full Text]  
  • Virbasius, J. V., Guilherme, A., Czech, M. P. (1996). Mouse p170 Is a Novel Phosphatidylinositol 3-Kinase Containing a C2 Domain. J. Biol. Chem. 271: 13304-13307 [Abstract] [Full Text]  
  • Klebanow, E. R., Poon, D., Zhou, S., Weil, P. A. (1996). Isolation and Characterization of TAF25, an Essential Yeast Gene That Encodes an RNA Polymerase II-specific TATA-binding Protein-associated Factor. J. Biol. Chem. 271: 13706-13715 [Abstract] [Full Text]  
  • Molz, L., Chen, Y.-W., Hirano, M., Williams, L. T. (1996). Cpk Is a Novel Class of Drosophila PtdIns 3-Kinase Containing a C2 Domain. J. Biol. Chem. 271: 13892-13899 [Abstract] [Full Text]  
  • Reaves, B., Bright, N., Mullock, B., Luzio, J. (1996). The effect of wortmannin on the localisation of lysosomal type I integral membrane glycoproteins suggests a role for phosphoinositide 3-kinase activity in regulating membrane traffic late in the endocytic pathway. J. Cell Sci. 109: 749-762 [Abstract]  
  • Takegawa, K, DeWald, D., Emr, S. (1995). Schizosaccharomyces pombe Vps34p, a phosphatidylinositol-specific PI 3-kinase essential for normal cell growth and vacuole morphology. J. Cell Sci. 108: 3745-3756 [Abstract]  
  • Stack, J.H., Herman, P.K., DeWald, D.B., Marcusson, E.G., Lin Cereghino, J., Horazdovsky, B.F., Emr, S.D. (1995). Novel Protein Kinase/Phosphatidylinositol 3-Kinase Complex Essential for Receptor-mediated Protein Sorting to the Vacuole in Yeast. Cold Spring Harb Symp Quant Biol 60: 157-170 [Abstract]  
  • Cowles, C., Emr, S., Horazdovsky, B. (1994). Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles. J. Cell Sci. 107: 3449-3459 [Abstract]  
  • Flanagan, C., Schnieders, E., Emerick, A., Kunisawa, R, Admon, A, Thorner, J (1993). Phosphatidylinositol 4-kinase: gene structure and requirement for yeast cell viability. Science 262: 1444-1448 [Abstract]  
  • Schu, P., Takegawa, K, Fry, M., Stack, J., Waterfield, M., Emr, S. (1993). Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. Science 260: 88-91 [Abstract]  
  • Shin, M. E., Ogburn, K. D., Varban, O. A., Gilbert, P. M., Burd, C. G. (2001). FYVE Domain Targets Pib1p Ubiquitin Ligase to Endosome and Vacuolar Membranes. J. Biol. Chem. 276: 41388-41393 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»