This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Goto, K Articles by Kondoh, H Search for Related Content PubMed PubMed Citation Articles by Goto, K Articles by Kondoh, H

 Previous Article  |  Next Article 

Mol Cell Biol. 1990 March; 10(3): 958-964

Functional cooperation of lens-specific and nonspecific elements in the delta 1-crystallin enhancer.

K Goto, T S Okada and H Kondoh

Department of Molecular Biology, Faculty of Science, Nagoya University, Japan.

ABSTRACT

The expression of the chicken delta 1-crystallin gene is primarily regulated by the action of a lens-specific enhancer 1 kilobase long and located in the third intron of the gene (S. Hayashi, K. Goto, T. S. Okada, and H. Kondoh, Genes Dev. 1:818-828, 1987). The 120-base-long core segment is required for the activity of the delta 1-crystallin enhancer but by itself shows no activity. We analyzed the action of the core and adjoining segments of the delta 1-crystallin enhancer by two different approaches: (i) multiplication of the segments to express any cryptic effect and (ii) competition among enhancers for nuclear factors involved in enhancer action. We found that (i) the core defines a strictly lens-specific element, (ii) an adjoining segment defines an element with a broad specificity with regard to cell type, (iii) these elements cooperate in cis within the delta 1-crystallin enhancer, (iv) the multimers of these elements complete with each other and with delta 1-crystallin and simian virus 40 enhancers in trans apparently without sequence specificity but in a fashion reflecting the strength of the enhancers, and (v) the enhancers in trans do not affect the expression of enhancer-free genes, thereby ruling out the possibility of competition for general transcription factors. The last two observations raise the possibility that the enhancer segments interacting with different sequence-specific factors also interact with one other component involved in enhancer action.

---

Mol Cell Biol. 1990 March; 10(3): 958-964

This article has been cited by other articles:

• Inoue, M., Kamachi, Y., Matsunami, H., Imada, K., Uchikawa, M., Kondoh, H. (2007). PAX6 and SOX2-dependent regulation of the Sox2 enhancer N-3 involved in embryonic visual system development. GENES CELLS 12: 1049-1061 [Abstract] [Full Text]  
• Tanaka, S., Kamachi, Y., Tanouchi, A., Hamada, H., Jing, N., Kondoh, H. (2004). Interplay of SOX and POU Factors in Regulation of the Nestin Gene in Neural Primordial Cells. Mol. Cell. Biol. 24: 8834-8846 [Abstract] [Full Text]  
• Kamachi, Y., Uchikawa, M., Tanouchi, A., Sekido, R., Kondoh, H. (2001). Pax6 and SOX2 form a co-DNA-binding partner complex that regulates initiation of lens development. Genes Dev. 15: 1272-1286 [Abstract] [Full Text]  
• Kamachi, Y, Uchikawa, M, Collignon, J, Lovell-Badge, R, Kondoh, H (1998). Involvement of Sox1, 2 and 3 in the early and subsequent molecular events of lens induction. Development 125: 2521-2532 [Abstract]  
• Funahashi, J, Sekido, R, Murai, K, Kamachi, Y, Kondoh, H (1993). Delta-crystallin enhancer binding protein delta EF1 is a zinc finger-homeodomain protein implicated in postgastrulation embryogenesis. Development 119: 433-446 [Abstract]