This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zimmerman, K
Right arrow Articles by Alt, F W
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zimmerman, K
Right arrow Articles by Alt, F W

 Previous Article  |  Next Article 

Mol Cell Biol. 1990 May; 10(5): 2096-2103

Differential regulation of the N-myc gene in transfected cells and transgenic mice.

K Zimmerman, E Legouy, V Stewart, R Depinho and F W Alt

Howard Hughes Medical Institute, New York, New York.

ABSTRACT

The N-myc gene is expressed specifically in the early developmental stages of numerous cell lineages. To assay for sequences that could potentially regulate N-myc expression, we transfected constructs that contained murine N-myc genomic sequences linked to a reporter gene and genomic clones that contained the complete human or murine N-myc genes into cell lines that either express or do not express the endogenous N-myc gene. Following either transient or stable transfection, the introduced N-myc sequences were expressed regardless of the expression status of the endogenous gene. In contrast, when the clones containing the complete human N-myc gene were introduced into the germline of transgenic mice, expression in some transgenic lines paralleled the tissue- and stage-specific expression of the endogenous murine gene. These findings demonstrate differences in the regulation of N-myc genes in recipient cells following in vitro versus in vivo introduction, suggesting that early developmental events may play a role in the regulation of N-myc expression.

Mol Cell Biol. 1990 May; 10(5): 2096-2103




This article has been cited by other articles:

  • Gostissa, M., Ranganath, S., Bianco, J. M., Alt, F. W. (2009). Chromosomal location targets different MYC family gene members for oncogenic translocations. Proc. Natl. Acad. Sci. USA 106: 2265-2270 [Abstract] [Full Text]  
  • Cai, C.-L., Zhou, W., Yang, L., Bu, L., Qyang, Y., Zhang, X., Li, X., Rosenfeld, M. G., Chen, J., Evans, S. (2005). T-box genes coordinate regional rates of proliferation and regional specification during cardiogenesis. Development 132: 2475-2487 [Abstract] [Full Text]  
  • Kramps, C., Strieder, V., Sapetschnig, A., Suske, G., Lutz, W. (2004). E2F and Sp1/Sp3 Synergize but Are Not Sufficient to Activate the MYCN Gene in Neuroblastomas. J. Biol. Chem. 279: 5110-5117 [Abstract] [Full Text]  
  • Kuzmanovic, M., Dudley, V. J., Sarthy, V. P. (2003). GFAP Promoter Drives Muller Cell-Specific Expression in Transgenic Mice. IOVS 44: 3606-3613 [Abstract] [Full Text]  
  • Wang, Y., Newton, D. C., Miller, T. L., Teichert, A.-M., Phillips, M. J., Davidoff, M. S., Marsden, P. A. (2002). An Alternative Promoter of the Human Neuronal Nitric Oxide Synthase Gene Is Expressed Specifically in Leydig Cells. Am. J. Pathol. 160: 369-380 [Abstract] [Full Text]  
  • Monroe, R. J., Chen, F., Ferrini, R., Davidson, L., Alt, F. W. (1999). RAG2 is regulated differentially in B and T cells by elements 5' of the promoter. Proc. Natl. Acad. Sci. USA 96: 12713-12718 [Abstract] [Full Text]  
  • Bell, A. W., Jiang, J.-G., Chen, Q., Liu, Y., Zarnegar, R. (1998). The Upstream Regulatory Regions of the Hepatocyte Growth Factor Gene Promoter Are Essential for Its Expression in Transgenic Mice. J. Biol. Chem. 273: 6900-6908 [Abstract] [Full Text]  
  • Chen, S., Zack, D. J. (1996). Ret 4, a Positive Acting Rhodopsin Regulatory Element Identified Using a Bovine Retina in Vitro Transcription System. J. Biol. Chem. 271: 28549-28557 [Abstract] [Full Text]  
  • Serra, R, Moses, H. (1995). pRb is necessary for inhibition of N-myc expression by TGF-beta 1 in embryonic lung organ cultures. Development 121: 3057-3066 [Abstract]  
  • Serra, R, Pelton, R., Moses, H. (1994). TGF beta 1 inhibits branching morphogenesis and N-myc expression in lung bud organ cultures. Development 120: 2153-2161 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»