This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vauti, F Articles by Nellen, W Search for Related Content PubMed PubMed Citation Articles by Vauti, F Articles by Nellen, W

 Previous Article  |  Next Article 

Mol Cell Biol. 1990 August; 10(8): 4080-4088

Regulation of the Discoidin I gamma gene in Dictyostelium discoideum: identification of individual promoter elements mediating induction of transcription and repression by cyclic AMP.

F Vauti, P Morandini, J Blusch, A Sachse and W Nellen

Max-Planck-Institut für Biochemie, Abt. Zellbiologie, Martinsried, Federal Republic of Germany.

ABSTRACT

We dissected the promoter of the developmentally induced and cyclic AMP-repressed discoidin I gamma gene and identified a sequence element essential for developmental induction. Transfer of the element to an inactive heterologous promoter demonstrated that this sequence is sufficient to confer expression in axenically growing cells and to induce gene activity in development after growth on bacteria. A 16-base-pair sequence within this element was shown to be sufficient for induction in the discoidin promoter context and was used to reactivate different truncated promoter constructs. This led to the localization of an element necessary for down regulation of gene expression by extracellular cyclic AMP.

---

Mol Cell Biol. 1990 August; 10(8): 4080-4088

This article has been cited by other articles:

• Strmecki, L., Bloomfield, G., Araki, T., Dalton, E., Skelton, J., Schilde, C., Harwood, A., Williams, J. G., Ivens, A., Pears, C. (2007). Proteomic and Microarray Analyses of the Dictyostelium Zak1-GSK-3 Signaling Pathway Reveal a Role in Early Development. Eukaryot Cell 6: 245-252 [Abstract] [Full Text]  
• Zhukovskaya, N. V., Fukuzawa, M., Yamada, Y., Araki, T., Williams, J. G. (2006). The Dictyostelium bZIP transcription factor DimB regulates prestalk-specific gene expression. Development 133: 439-448 [Abstract] [Full Text]  
• Zhukovskaya,, N. V., Fukuzawa,, M., Tsujioka, M., Jermyn, K. A., Kawata, T., Abe, T., Zvelebil, M., Williams, J. G. (2004). Dd-STATb, a Dictyostelium STAT protein with a highly aberrant SH2 domain, functions as a regulator of gene expression during growth and early development. Development 131: 447-458 [Abstract] [Full Text]  
• Zeng, C., Anjard, C., Riemann, K., Konzok, A., Nellen, W. (2000). gdt1, a New Signal Transduction Component for Negative Regulation of the Growth-Differentiation Transition in Dictyostelium discoideum. Mol. Biol. Cell 11: 1631-1643 [Abstract] [Full Text]  
• Bonfils, C., Gaudet, P., Tsang, A. (1999). Identification of cis-Regulating Elements and trans-Acting Factors Regulating the Expression of the Gene Encoding the Small Subunit of Ribonucleotide Reductase in Dictyostelium discoideum. J. Biol. Chem. 274: 20384-20390 [Abstract] [Full Text]  
• Reymond, C. D., Beghdadi-Rais, C., Roggero, M., Duarte, E. A., Desponds, C., Bernard, M., Groux, D., Matile, H., Bron, C., Corradin, G., Fasel, N. J. (1995). Anchoring of an Immunogenic Plasmodium falciparum Circumsporozoite Protein on the Surface of Dictyostelium discoideum. J. Biol. Chem. 270: 12941-12947 [Abstract] [Full Text]  
• Anjard, C, Pinaud, S, Kay, R., Reymond, C. (1992). Overexpression of Dd PK2 protein kinase causes rapid development and affects the intracellular cAMP pathway of Dictyostelium discoideum. Development 115: 785-790 [Abstract]