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Mutational analysis of the functional domains of yeast K1 killer toxin.

Department of Biology, McGill University, Montreal, Quebec, Canada.

ABSTRACT

To determine the functional domains of K1 killer toxin, we analyzed the phenotypes of a set of mutations throughout regions encoding the alpha- and beta-toxin subunits that allow secretion of mutant toxins. A range of techniques have been used to examine the ability of mutant toxins to bind to beta-glucan cell wall receptor and to form lethal ion channels. Our results indicate that both the alpha and beta subunits are involved in beta-glucan receptor binding. Defects in ion channel formation and toxin immunity are confined to the hydrophobic alpha subunit of the toxin.

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• Suzuki, C., Kawano, M., Kashiwagi, T., Arata, Y., Kawasumi, T., Kashiwagi, Y. (2000). Lethal effect of the expression of a killer gene SMK1 in Saccharomyces cerevisiae. Protein Eng Des Sel 13: 73-76 [Abstract] [Full Text]