This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shtivelman, E
Right arrow Articles by Bishop, J M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shtivelman, E
Right arrow Articles by Bishop, J M

 Previous Article  |  Next Article 

Mol Cell Biol. 1991 November; 11(11): 5446-5453

Expression of CD44 is repressed in neuroblastoma cells.

E Shtivelman and J M Bishop

Department of Microbiology and Immunology, University of California, San Francisco 94143.

ABSTRACT

We have used cDNA subtractive cloning to identify a group of human genes that are expressed in diverse differentiated derivatives of neural crest origin but not in neuroblastoma cell lines. One of these genes was identified as CD44, which encodes an integral membrane glycoprotein that serves as the principal receptor for hyaluronate and participates in specific cell-cell and cell-extracellular matrix interactions. The repression of CD44 expression in neuroblastoma cell lines might be relevant to their high metastatic potential. We have cloned full-length cDNAs corresponding to CD44 trancscripts and identified a novel splice variant of CD44 lacking 31 amino acids of the extracellular domain. As a first step toward analysis of CD44 downregulation in neuroblastoma cells, we have mapped the CD44 RNA initiation site and analyzed the structure of the upstream regulatory region. We constructed a series of plasmids containing different amounts of CD44 upstream regulatory region linked to the bacterial chloramphenicol acetyltransferase gene and then analyzed their ability to promote transcription in neuroblastoma and melanoma cells. We found that a DNA segment including about 150 bp of the CD44 upstream region and the 5' end of the gene itself was sufficient to induce substantial transcription of the chloramphenicol acetyltransferase gene in both neuroblastoma and melanoma cells. Several upstream cis-acting elements contribute to the downregulation of CD44 in neuroblastoma cells, the most prominent being a 120-bp DNA fragment located 450 bp upstream to the RNA initiation site. Our data suggest that multiple factors might be involved in downregulation of CD44 in neuroblastoma cells.

Mol Cell Biol. 1991 November; 11(11): 5446-5453




This article has been cited by other articles:

  • Balla, M. M. S., Vemuganti, G. K., Kannabiran, C., Honavar, S. G., Murthy, R. (2009). Phenotypic Characterization of Retinoblastoma for the Presence of Putative Cancer Stem-like Cell Markers by Flow Cytometry. IOVS 50: 1506-1514 [Abstract] [Full Text]  
  • Wu, G. D., Wang, H., Zhu, H., He, Y., Barr, M. L., Klein, A. S. (2008). Genetic Modulation of CD44 Expression by Intragraft Fibroblasts. J Biochem 144: 571-580 [Abstract] [Full Text]  
  • Lopez, J. I., Camenisch, T. D., Stevens, M. V., Sands, B. J., McDonald, J., Schroeder, J. A. (2005). CD44 Attenuates Metastatic Invasion during Breast Cancer Progression. Cancer Res. 65: 6755-6763 [Abstract] [Full Text]  
  • Hendricks, K. B., Shanahan, F., Lees, E. (2004). Role for BRG1 in Cell Cycle Control and Tumor Suppression. Mol. Cell. Biol. 24: 362-376 [Abstract] [Full Text]  
  • Hebbard, L, Steffen, A, Zawadzki, V, Fieber, C, Howells, N, Moll, J, Ponta, H, Hofmann, M, Sleeman, J (2000). CD44 expression and regulation during mammary gland development and function. J. Cell Sci. 113: 2619-2630 [Abstract]  
  • Protin, U., Schweighoffer, T., Jochum, W., Hilberg, F. (1999). CD44-Deficient Mice Develop Normally with Changes in Subpopulations and Recirculation of Lymphocyte Subsets. J. Immunol. 163: 4917-4923 [Abstract] [Full Text]  
  • Maris, J. M., Matthay, K. K. (1999). Molecular Biology of Neuroblastoma. JCO 17: 2264-2264 [Abstract] [Full Text]  
  • Lou, W., Krill, D., Dhir, R., Becich, M. J., Dong, J.-T., Frierson, H. F. Jr., Isaacs, W. B., Isaacs, J. T., Gao, A. C. (1999). Methylation of the CD44 Metastasis Suppressor Gene in Human Prostate Cancer. Cancer Res. 59: 2329-2331 [Abstract] [Full Text]  
  • Fitzgerald, K. A., O'Neill, L. A. J. (1999). Characterization of CD44 Induction by IL-1: A Critical Role for Egr-1. J. Immunol. 162: 4920-4927 [Abstract] [Full Text]  
  • Schmits, R., Filmus, J., Gerwin, N., Senaldi, G., Kiefer, F., Kundig, T., Wakeham, A., Shahinian, A., Catzavelos, C., Rak, J., Furlonger, C., Zakarian, A., Simard, J. J.L., Ohashi, P. S., Paige, C. J., Gutierrez-Ramos, J. C., Mak, T. W. (1997). CD44 Regulates Hematopoietic Progenitor Distribution, Granuloma Formation, and Tumorigenicity. Blood 90: 2217-2233 [Abstract] [Full Text]  
  • Zhang, M., Wang, M. H., Singh, R. K., Wells, A., Siegal, G. P. (1997). Epidermal Growth Factor Induces CD44 Gene Expression through a Novel Regulatory Element in Mouse Fibroblasts. J. Biol. Chem. 272: 14139-14146 [Abstract] [Full Text]  
  • Hudson, D., Sleeman, J, Watt, F. (1995). CD44 is the major peanut lectin-binding glycoprotein of human epidermal keratinocytes and plays a role in intercellular adhesion. J. Cell Sci. 108: 1959-1970 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»