This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hino, M Articles by Shibuya, M Search for Related Content PubMed PubMed Citation Articles by Hino, M Articles by Shibuya, M

 Previous Article  |  Next Article 

Mol Cell Biol. 1991 November; 11(11): 5527-5533

Unregulated expression of the erythropoietin receptor gene caused by insertion of spleen focus-forming virus long terminal repeat in a murine erythroleukemia cell line.

Department of Genetics, University of Tokyo, Japan.

ABSTRACT

A murine erythroleukemia (MEL) cell line, F5-5, expressed 10,000 binding sites for erythropoietin (EPO) per cell, 10-fold more than was expressed by other murine erythroleukemia cell lines and normal erythroid progenitors. Northern (RNA) and Southern blot analyses revealed overexpression of mRNA for the EPO receptor (EPOR) and rearrangement of one of the EPOR gene alleles in F5-5 cells, respectively. Molecular cloning of F5-5-derived cDNA encoding EPOR revealed that the 5' noncoding region of the EPOR cDNA corresponds to the 3' long terminal repeat sequence of the polycythemic strain of Friend spleen focus-forming virus (F-SFFVP). The aberrant EPOR transcripts containing the 3' long terminal repeat sequence were mainly expressed in F5-5 cells. The same integration upstream of the EPOR gene was also observed in other subclones and the parent cell line. It is possible that overexpression of EPOR by viral promoter insertion will confer growth advantage to an F-SFFVP-infected erythroid progenitor cell, leading to positive clonal selection through further leukemogenic steps.

This article has been cited by other articles:

• Ney, P. A., D'Andrea, A. D. (2000). Friend erythroleukemia revisited. Blood 96: 3675-3680 [Full Text]  
• Gonda, T. J., D'Andrea, R. J. (1997). Activating Mutations in Cytokine Receptors: Implications for Receptor Function and Role in Disease. Blood 89: 355-369 [Full Text]