This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Virbasius, J V Articles by Scarpulla, R C Search for Related Content PubMed PubMed Citation Articles by Virbasius, J V Articles by Scarpulla, R C

 Previous Article  |  Next Article 

Mol Cell Biol. 1991 November; 11(11): 5631-5638

Transcriptional activation through ETS domain binding sites in the cytochrome c oxidase subunit IV gene.

Department of Cell, Molecular and Structural Biology, Northwestern University Medical School, Chicago, Illinois 60611.

ABSTRACT

A mutational analysis of the rat cytochrome c oxidase subunit IV (RCO4) promoter region revealed the presence of a major control element consisting of a tandemly repeated pair of binding sites for a nuclear factor from HeLa cells. This factor was designated NRF-2 (nuclear respiratory factor 2) because a functional recognition site was also found in the human ATP synthase beta-subunit gene. Deletion or site-directed point mutations of the NRF-2 binding sites in the RCO4 promoter resulted in substantial loss of transcriptional activity, and synthetic oligomers of the NRF-2 binding sites from both genes stimulated a heterologous promoter when cloned in cis. NRF-2 binding and transcriptional activation required a purine-rich core sequence, GGAA. This motif is characteristic of the recognition site for a family of activators referred to as ETS domain proteins because of the similarity within their DNA-binding domains to the ets-1 proto-oncogene product. NRF-2 recognized an authentic Ets-1 site within the Moloney murine sarcoma virus long terminal repeat, and this site was able to compete for NRF-2 binding to the RCO4 promoter sequence. In addition, a single polypeptide of 55 kDa was detected following cross-linking of a partially purified NRF-2 fraction to RCO4, the human ATP synthase beta subunit, or Moloney murine sarcoma virus binding sites. However, in contrast to Ets-1, which appears to be exclusive to lymphoid tissues, NRF-2 has the broad tissue distribution expected of a regulator of respiratory chain expression.

This article has been cited by other articles:

• Scarpulla, R. C. (2008). Transcriptional Paradigms in Mammalian Mitochondrial Biogenesis and Function. Physiol. Rev. 88: 611-638 [Abstract] [Full Text]  
• Dhar, S. S., Ongwijitwat, S., Wong-Riley, M. T. T. (2008). Nuclear Respiratory Factor 1 Regulates All Ten Nuclear-encoded Subunits of Cytochrome c Oxidase in Neurons. J. Biol. Chem. 283: 3120-3129 [Abstract] [Full Text]  
• Zanotto, E., Shah, Z. H., Jacobs, H. T. (2007). The bidirectional promoter of two genes for the mitochondrial translational apparatus in mouse is regulated by an array of CCAAT boxes interacting with the transcription factor NF-Y. Nucleic Acids Res 35: 664-677 [Abstract] [Full Text]  
• Berdanier, C. D. (2006). Mitochondrial gene expression: influence of nutrients and hormones.. Exp. Biol. Med. 231: 1593-1601 [Abstract] [Full Text]  
• Angus, L. M., Chakkalakal, J. V., Mejat, A., Eibl, J. K., Belanger, G., Megeney, L. A., Chin, E. R., Schaeffer, L., Michel, R. N., Jasmin, B. J. (2005). Calcineurin-NFAT signaling, together with GABP and peroxisome PGC-1{alpha}, drives utrophin gene expression at the neuromuscular junction. Am. J. Physiol. Cell Physiol. 289: C908-C917 [Abstract] [Full Text]  
• Cartoni, R., Leger, B., Hock, M. B., Praz, M., Crettenand, A., Pich, S., Ziltener, J.-L., Luthi, F., Deriaz, O., Zorzano, A., Gobelet, C., Kralli, A., Russell, A. P (2005). Mitofusins 1/2 and ERR{alpha} expression are increased in human skeletal muscle after physical exercise. J. Physiol. 567: 349-358 [Abstract] [Full Text]  
• Gleyzer, N., Vercauteren, K., Scarpulla, R. C. (2005). Control of Mitochondrial Transcription Specificity Factors (TFB1M and TFB2M) by Nuclear Respiratory Factors (NRF-1 and NRF-2) and PGC-1 Family Coactivators. Mol. Cell. Biol. 25: 1354-1366 [Abstract] [Full Text]  
• Kelly, D. P., Scarpulla, R. C. (2004). Transcriptional regulatory circuits controlling mitochondrial biogenesis and function. Genes Dev. 18: 357-368 [Full Text]  
• Stumpferl, S. W., Stephan, O., Osiewacz, H. D. (2004). Impact of a Disruption of a Pathway Delivering Copper to Mitochondria on Podospora anserina Metabolism and Life Span. Eukaryot Cell 3: 200-211 [Abstract] [Full Text]  
• Imaki, H., Nakayama, K., Delehouzee, S., Handa, H., Kitagawa, M., Kamura, T., Nakayama, K. I. (2003). Cell Cycle-dependent Regulation of the Skp2 Promoter by GA-binding Protein. Cancer Res. 63: 4607-4613 [Abstract] [Full Text]  
• Chinenov, Y., Henzl, M., Martin, M. E. (2000). The alpha and beta Subunits of the GA-binding Protein Form a Stable Heterodimer in Solution. REVISED MODEL OF HETEROTETRAMERIC COMPLEX ASSEMBLY. J. Biol. Chem. 275: 7749-7756 [Abstract] [Full Text]  
• Arnaud, E., Nicaud, V., Poirier, O., Rendu, F., Alhenc-Gelas, M., Fiessinger, J.-N., Emmerich, J., Aiach, M. (2000). Protective Effect of a Thrombin Receptor (Protease-Activated Receptor 1) Gene Polymorphism Toward Venous Thromboembolism. Arterioscler. Thromb. Vasc. Bio. 20: 585-592 [Abstract] [Full Text]  
• Bannert, N., Avots, A., Baier, M., Serfling, E., Kurth, R. (1999). GA-binding protein factors, in concert with the coactivator CREB binding protein/p300, control the induction of the interleukin 16 promoter in T lymphocytes. Proc. Natl. Acad. Sci. USA 96: 1541-1546 [Abstract] [Full Text]  
• Yu, M., Yang, X.-Y., Schmidt, T., Chinenov, Y., Wang, R., Martin, M. E. (1997). GA-binding Protein-dependent Transcription Initiator Elements. EFFECT OF HELICAL SPACING BETWEEN POLYOMAVIRUS ENHANCER A FACTOR 3(PEA3)/Ets-BINDING SITES ON INITIATOR ACTIVITY. J. Biol. Chem. 272: 29060-29067 [Abstract] [Full Text]  
• Gugneja, S., Scarpulla, R. C. (1997). Serine Phosphorylation within a Concise Amino-terminal Domain in Nuclear Respiratory Factor 1 Enhances DNA Binding. J. Biol. Chem. 272: 18732-18739 [Abstract] [Full Text]  
• Kamura, T., Handa, H., Hamasaki, N., Kitajima, S. (1997). Characterization of the Human Thrombopoietin Gene Promoter. A POSSIBLE ROLE OF AN Ets TRANSCRIPTION FACTOR, E4TF1/GABP. J. Biol. Chem. 272: 11361-11368 [Abstract] [Full Text]  
• Seelan, R. S., Grossman, L. I. (1997). Structural Organization and Promoter Analysis of the Bovine Cytochrome c Oxidase Subunit VIIc Gene. A FUNCTIONAL ROLE FOR YY1. J. Biol. Chem. 272: 10175-10181 [Abstract] [Full Text]  
• Martin, M. E., Chinenov, Y., Yu, M., Schmidt, T. K., Yang, X.-Y. (1996). Redox Regulation of GA-binding Protein-alpha DNA Binding Activity. J. Biol. Chem. 271: 25617-25623 [Abstract] [Full Text]  
• Li, R., Hodny, Z., Luciakova, K., Barath, P., Nelson, B. D. (1996). Sp1 Activates and Inhibits Transcription from Separate Elements in the Proximal Promoter of the Human Adenine Nucleotide Translocase 2(ANT2) Gene. J. Biol. Chem. 271: 18925-18930 [Abstract] [Full Text]  
• Seelan, R. S., Gopalakrishnan, L., Scarpulla, R. C., Grossman, L. I. (1996). Cytochrome c Oxidase Subunit VIIa Liver Isoform. J. Biol. Chem. 271: 2112-2120 [Abstract] [Full Text]  
• Albanese, C., Johnson, J., Watanabe, G., Eklund, N., Vu, D., Arnold, A., Pestell, R. G. (1995). Transforming p21[IMAGE] Mutants and c-Ets-2 Activate the Cyclin D1 Promoter through Distinguishable Regions. J. Biol. Chem. 270: 23589-23597 [Abstract] [Full Text]  
• Gopalakrishnan, L., Scarpulla, R. C. (1995). Structure, Expression, and Chromosomal Assignment of the Human Gene Encoding Nuclear Respiratory Factor 1. J. Biol. Chem. 270: 18019-18025 [Abstract] [Full Text]  
• Izquierdo, J. M., Ricart, J., Ostronoff, L. K., Egea, G., Cuezva, J. M. (1995). Changing Patterns of Transcriptional and Post-transcriptional Control of [IMAGE]-F[IMAGE]-ATPase Gene Expression during Mitochondrial Biogenesis in Liver. J. Biol. Chem. 270: 10342-10350 [Abstract] [Full Text]  
• Houstek, J., Andersson, U., Tvrdík, P., Nedergaard, J., Cannon, B. (1995). The Expression of Subunit c Correlates with and Thus May Limit the Biosynthesis of the Mitochondrial F(0)F(1)-ATPase in Brown Adipose Tissue. J. Biol. Chem. 270: 7689-7694 [Abstract] [Full Text]  
• Dameron, K., Volpert, O., Tainsky, M., Bouck, N (1994). Control of angiogenesis in fibroblasts by p53 regulation of thrombospondin-1. Science 265: 1582-1584 [Abstract]  
• Virbasius, C A, Virbasius, J V, Scarpulla, R C (1993). NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of developmental regulators.. Genes Dev. 7: 2431-2445 [Abstract]  
• Virbasius, J V, Virbasius, C A, Scarpulla, R C (1993). Identity of GABP with NRF-2, a multisubunit activator of cytochrome oxidase expression, reveals a cellular role for an ETS domain activator of viral promoters.. Genes Dev. 7: 380-392 [Abstract]  
• Gong, Q., Brown, L. J., MacDonald, M. J. (2000). Functional Analysis of Two Promoters for the Human Mitochondrial Glycerol Phosphate Dehydrogenase Gene. J. Biol. Chem. 275: 38012-38021 [Abstract] [Full Text]