This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Moritz, M Articles by Woolford, J L Search for Related Content PubMed PubMed Citation Articles by Moritz, M Articles by Woolford, J L, Jr

Assembly of 60S ribosomal subunits is perturbed in temperature-sensitive yeast mutants defective in ribosomal protein L16.

Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213.

ABSTRACT

Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two heat-sensitive and two cold-sensitive isolates were characterized. The growth of the four mutants is inhibited at their restrictive temperatures. However, many of the cells remain viable if returned to their permissive temperatures. All of the mutants are deficient in 60S ribosomal subunits and therefore accumulate translational preinitiation complexes. Three of the mutants exhibit a shortage of mature 25S rRNA, and one accumulates rRNA precursors. The accumulation of rRNA precursors suggests that ribosome assembly may be slowed in this mutant. These phenotypes lead us to propose that mutants containing the rpl16b alleles are defective for 60S subunit assembly rather than function. In the mutant carrying the rpl16b-1 allele, ribosomes initiate translation at the noncanonical codon AUA, at least on the rpl16b-1 mRNA, bringing to light a possible connection between the rate and the fidelity of translation initiation.

This article has been cited by other articles:

• Abe, F., Minegishi, H. (2008). Global Screening of Genes Essential for Growth in High-Pressure and Cold Environments: Searching for Basic Adaptive Strategies Using a Yeast Deletion Library. Genetics 178: 851-872 [Abstract] [Full Text]  
• Chaudhuri, S., Vyas, K., Kapasi, P., Komar, A. A., Dinman, J. D., Barik, S., Mazumder, B. (2007). Human ribosomal protein L13a is dispensable for canonical ribosome function but indispensable for efficient rRNA methylation. RNA 13: 2224-2237 [Abstract] [Full Text]  
• Martin-Marcos, P., Hinnebusch, A. G., Tamame, M. (2007). Ribosomal Protein L33 Is Required for Ribosome Biogenesis, Subunit Joining, and Repression of GCN4 Translation. Mol. Cell. Biol. 27: 5968-5985 [Abstract] [Full Text]  
• Rosado, I. V., Kressler, D., Cruz, J. d. l. (2007). Functional analysis of Saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis. Nucleic Acids Res 0: gkm388v1-11 [Abstract] [Full Text]  
• Rosado, I. V., Dez, C., Lebaron, S., Caizergues-Ferrer, M., Henry, Y., de la Cruz, J. (2007). Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) Reveals a Low-Molecular-Mass Complex Containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p Involved in Early Steps of 60S Ribosomal Subunit Biogenesis. Mol. Cell. Biol. 27: 1207-1221 [Abstract] [Full Text]  
• O'Connor, M., Gregory, S. T., Dahlberg, A. E. (2004). Multiple defects in translation associated with altered ribosomal protein L4. Nucleic Acids Res 32: 5750-5756 [Abstract] [Full Text]  
• Miyoshi, K., Tsujii, R., Yoshida, H., Maki, Y., Wada, A., Matsui, Y., Toh-e, A., Mizuta, K. (2002). Normal Assembly of 60 S Ribosomal Subunits Is Required for the Signaling in Response to a Secretory Defect in Saccharomyces cerevisiae. J. Biol. Chem. 277: 18334-18339 [Abstract] [Full Text]  
• Moy, T. I., Boettner, D., Rhodes, J. C., Silver, P. A., Askew, D. S. (2002). Identification of a role for Saccharomyces cerevisiae Cgr1p in pre-rRNA processing and 60S ribosome subunit synthesis. Microbiology 148: 1081-1090 [Abstract] [Full Text]  
• Gadal, O., Strauß, D., Kessl, J., Trumpower, B., Tollervey, D., Hurt, E. (2001). Nuclear Export of 60S Ribosomal Subunits Depends on Xpo1p and Requires a Nuclear Export Sequence-Containing Factor, Nmd3p, That Associates with the Large Subunit Protein Rpl10p. Mol. Cell. Biol. 21: 3405-3415 [Abstract] [Full Text]  
• Basu, U., Si, K., Warner, J. R., Maitra, U. (2001). The Saccharomyces cerevisiae TIF6 Gene Encoding Translation Initiation Factor 6 Is Required for 60S Ribosomal Subunit Biogenesis. Mol. Cell. Biol. 21: 1453-1462 [Abstract] [Full Text]  
• Strezoska, Z., Pestov, D. G., Lau, L. F. (2000). Bop1 Is a Mouse WD40 Repeat Nucleolar Protein Involved in 28S and 5.8S rRNA Processing and 60S Ribosome Biogenesis. Mol. Cell. Biol. 20: 5516-5528 [Abstract] [Full Text]  
• Tsuno, A., Miyoshi, K., Tsujii, R., Miyakawa, T., Mizuta, K. (2000). RRS1, a Conserved Essential Gene, Encodes a Novel Regulatory Protein Required for Ribosome Biogenesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 2066-2074 [Abstract] [Full Text]  
• Kressler, D., Linder, P., de la Cruz, J. (1999). Protein trans-Acting Factors Involved in Ribosome Biogenesis in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 7897-7912 [Full Text]  
• Zebarjadian, Y., King, T., Fournier, M. J., Clarke, L., Carbon, J. (1999). Point Mutations in Yeast CBF5 Can Abolish In Vivo Pseudouridylation of rRNA. Mol. Cell. Biol. 19: 7461-7472 [Abstract] [Full Text]  
• Dechampesme, A.-M., Koroleva, O., Leger-Silvestre, I., Gas, N., Camier, S. (1999). Assembly of 5S Ribosomal RNA Is Required at a Specific Step of the Pre-rRNA Processing Pathway. J. Cell Biol. 145: 1369-1380 [Abstract] [Full Text]  
• Ho, J. H.-N., Johnson, A. W. (1999). NMD3 Encodes an Essential Cytoplasmic Protein Required for Stable 60S Ribosomal Subunits in Saccharomyces cerevisiae. Mol. Cell. Biol. 19: 2389-2399 [Abstract] [Full Text]  
• Si, K., Maitra, U. (1999). The Saccharomyces cerevisiae Homologue of Mammalian Translation Initiation Factor 6 Does Not Function as a Translation Initiation Factor. Mol. Cell. Biol. 19: 1416-1426 [Abstract] [Full Text]  
• Zanchin, N. I. T., Goldfarb, D. S. (1999). Nip7p Interacts with Nop8p, an Essential Nucleolar Protein Required for 60S Ribosome Biogenesis, and the Exosome Subunit Rrp43p. Mol. Cell. Biol. 19: 1518-1525 [Abstract] [Full Text]  
• Kressler, D., de la Cruz, J., Rojo, M., Linder, P. (1998). Dbp6p Is an Essential Putative ATP-Dependent RNA Helicase Required for 60S-Ribosomal-Subunit Assembly in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 1855-1865 [Abstract] [Full Text]  
• Rosenblum, J. S., Pemberton, L. F., Blobel, G. (1997). A Nuclear Import Pathway for a Protein Involved in tRNA Maturation. J. Cell Biol. 139: 1655-1661 [Abstract] [Full Text]  
• Proweller, A., Butler, J. S. (1997). Ribosome Concentration Contributes to Discrimination against Poly(A)- mRNA during Translation Initiation in Saccharomyces cerevisiae. J. Biol. Chem. 272: 6004-6010 [Abstract] [Full Text]  
• Martin-Nieto, J, Roufa, D. (1997). Functional analysis of human RPS14 null alleles. J. Cell Sci. 110: 955-963 [Abstract]  
• Proweller, A., Butler, J. S. (1996). Ribosomal Association of Poly(A)-binding Protein in Poly(A)-deficient Saccharomyces cerevisiae. J. Biol. Chem. 271: 10859-10865 [Abstract] [Full Text]  
• Deshmukh, M., Stark, J., Yeh, L.-C. C., Lee, J. C., Woolford, J. L. Jr. (1995). Multiple Regions of Yeast Ribosomal Protein L1 Are Important for Its Interaction with 5 S rRNA and Assembly into Ribosomes. J. Biol. Chem. 270: 30148-30156 [Abstract] [Full Text]  
• Barker, D D, Wang, C, Moore, J, Dickinson, L K, Lehmann, R (1992). Pumilio is essential for function but not for distribution of the Drosophila abdominal determinant Nanos.. Genes Dev. 6: 2312-2326 [Abstract]