This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Medema, R H Articles by Bos, J L Search for Related Content PubMed PubMed Citation Articles by Medema, R H Articles by Bos, J L

 Previous Article  |  Next Article 

Mol Cell Biol. 1991 December; 11(12): 5963-5967

Two dominant inhibitory mutants of p21ras interfere with insulin-induced gene expression.

Department of Physiological Chemistry, University of Utrecht, The Netherlands.

ABSTRACT

Insulin induces a rapid activation of p21ras in NIH 3T3 and Chinese hamster ovary cells that overexpress the insulin receptor. Previously, we suggested that p21ras may mediate insulin-induced gene expression. To test such a function of p21ras more directly, we studied the effect of different dominant inhibitory mutants of p21ras on the induction of gene expression in response to insulin. We transfected a collagenase promoter-chloramphenicol acetyltransferase (CAT) gene or a fos promoter-luciferase gene into NIH 3T3 cells that overexpressed the insulin receptor. The activities of both promoters were strongly induced after treatment with insulin. This induction could be suppressed by cotransfection of two inhibitory mutant ras genes, H-ras(Asn-17) or H-ras(Leu-61,Ser-186). In particular, insulin-induced activation of the fos promoter was inhibited completely by H-ras(Asn-17). These results show that p21ras functions as an intermediate in the insulin signal transduction route leading to the induction of gene expression.

This article has been cited by other articles:

• Hollestelle, M. J, Timmerman, P., Meloen, R. H, Hoppener, J. W M (2008). Characterization of gastrin-cholecystokinin 2 receptor interaction in relation to c-fos induction. Endocr Relat Cancer 15: 301-309 [Abstract] [Full Text]  
• de Gorter, D. J. J., Vos, J. C. M., Pals, S. T., Spaargaren, M. (2007). The B Cell Antigen Receptor Controls AP-1 and NFAT Activity through Ras-Mediated Activation of Ral. J. Immunol. 178: 1405-1414 [Abstract] [Full Text]  
• Morrison, H., Sperka, T., Manent, J., Giovannini, M., Ponta, H., Herrlich, P. (2007). Merlin/Neurofibromatosis Type 2 Suppresses Growth by Inhibiting the Activation of Ras and Rac. Cancer Res. 67: 520-527 [Abstract] [Full Text]  
• De Ruiter, N. D., Burgering, B. M. T., Bos, J. L. (2001). Regulation of the Forkhead Transcription Factor AFX by Ral-Dependent Phosphorylation of Threonines 447 and 451. Mol. Cell. Biol. 21: 8225-8235 [Abstract] [Full Text]  
• Wasylyk, C., Wasylyk, B. (2000). Defect in the p53-Mdm2 Autoregulatory Loop Resulting from Inactivation of TAFII250 in Cell Cycle Mutant tsBN462 Cells. Mol. Cell. Biol. 20: 5554-5570 [Abstract] [Full Text]  
• Reedquist, K. A., Ross, E., Koop, E. A., Wolthuis, R. M.F., Zwartkruis, F. J.T., van Kooyk, Y., Salmon, M., Buckley, C. D., Bos, J. L. (2000). The Small GTPase, Rap1, Mediates CD31-induced Integrin Adhesion. J. Cell Biol. 148: 1151-1158 [Abstract] [Full Text]  
• GIORDANO, S., MAFFE, A., WILLIAMS, T. A., ARTIGIANI, S., GUAL, P., BARDELLI, A., BASILICO, C., MICHIELI, P., COMOGLIO, P. M. (2000). Different point mutations in the met oncogene elicit distinct biological properties. FASEB J. 14: 399-406 [Abstract] [Full Text]  
• Voz, M. L., Agten, N. S., Van de Ven, W. J. M., Kas, K. (2000). PLAG1, the Main Translocation Target in Pleomorphic Adenoma of the Salivary Glands, Is a Positive Regulator of IGF-II. Cancer Res. 60: 106-113 [Abstract] [Full Text]  
• Vanoni, M., Bertini, R., Sacco, E., Fontanella, L., Rieppi, M., Colombo, S., Martegani, E., Carrera, V., Moroni, A., Bizzarri, C., Sabbatini, V., Cattozzo, M., Colagrande, A., Alberghina, L. (1999). Characterization and Properties of Dominant-negative Mutants of the Ras-specific Guanine Nucleotide Exchange Factor CDC25Mm. J. Biol. Chem. 274: 36656-36662 [Abstract] [Full Text]  
• Vo{beta}, M., Weernink, P. A. O., Haupenthal, S., Moller, U., Cool, R. H., Bauer, B., Camonis, J. H., Jakobs, K. H., Schmidt, M. (1999). Phospholipase D Stimulation by Receptor Tyrosine Kinases Mediated by Protein Kinase C and a Ras/Ral Signaling Cascade. J. Biol. Chem. 274: 34691-34698 [Abstract] [Full Text]  
• Spaargaren, M., Bos, J. L. (1999). Rab5 Induces Rac-independent Lamellipodia Formation and Cell Migration. Mol. Biol. Cell 10: 3239-3250 [Abstract] [Full Text]  
• Misra, U. K., Gawdi, G., Gonzalez-Gronow, M., Pizzo, S. V. (1999). Coordinate Regulation of the alpha 2-Macroglobulin Signaling Receptor and the Low Density Lipoprotein Receptor-related Protein/alpha 2-Macroglobulin Receptor by Insulin. J. Biol. Chem. 274: 25785-25791 [Abstract] [Full Text]  
• Chapman, S. C., Ayala, J. E., Streeper, R. S., Culbert, A. A., Eaton, E. M., Svitek, C. A., Goldman, J. K., Tavare, J. M., O'Brien, R. M. (1999). Multiple Promoter Elements Are Required for the Stimulatory Effect of Insulin on Human Collagenase-1 Gene Transcription. SELECTIVE EFFECTS ON ACTIVATOR PROTEIN-1 EXPRESSION MAY EXPLAIN THE QUANTITATIVE DIFFERENCE IN INSULIN AND PHORBOL ESTER ACTION. J. Biol. Chem. 274: 18625-18634 [Abstract] [Full Text]  
• Verheijen, M. H. G., Wolthuis, R. M. F., Bos, J. L., Defize, L. H. K. (1999). The Ras/Erk Pathway Induces Primitive Endoderm but Prevents Parietal Endoderm Differentiation of F9 Embryonal Carcinoma Cells. J. Biol. Chem. 274: 1487-1494 [Abstract] [Full Text]  
• Streeper, R. S., Chapman, S. C., Ayala, J. E., Svitek, C. A., Goldman, J. K., Cave, A., O’Brien, R. M. (1998). A Phorbol Ester-Insensitive AP-1 Motif Mediates the Stimulatory Effect of Insulin on Rat Malic Enzyme Gene Transcription. Mol. Endocrinol. 12: 1778-1791 [Abstract] [Full Text]  
• Yeagley, D., Agati, J. M., Quinn, P. G. (1998). A Tripartite Array of Transcription Factor Binding Sites Mediates cAMP Induction of Phosphoenolpyruvate Carboxykinase Gene Transcription and Its Inhibition by Insulin. J. Biol. Chem. 273: 18743-18750 [Abstract] [Full Text]  
• Patrone, C., Gianazza, E., Santagati, S., Agrati, P., Maggi, A. (1998). Divergent Pathways Regulate Ligand-Independent Activation of ER{alpha} in SK-N-BE Neuroblastoma and COS-1 Renal Carcinoma Cells. Mol. Endocrinol. 12: 835-841 [Abstract] [Full Text]  
• Zhao, H., Okada, S., Pessin, J. E., Koretzky, G. A. (1998). Insulin Receptor-mediated Dissociation of Grb2 from Sos Involves Phosphorylation of Sos by Kinase(s) Other than Extracellular Signal-regulated Kinase. J. Biol. Chem. 273: 12061-12067 [Abstract] [Full Text]  
• Harada, S., Smith, R. M., Smith, J. A., White, M. F., Jarett, L. (1996). Insulin-induced egr-1 and c-fos Expression in 32D Cells Requires Insulin Receptor, Shc, and Mitogen-activated Protein Kinase, but Not Insulin Receptor Substrate-1 and Phosphatidylinositol 3-Kinase Activation. J. Biol. Chem. 271: 30222-30226 [Abstract] [Full Text]  
• Fixman, E. D., Fournier, T. M., Kamikura, D. M., Naujokas, M. A., Park, M. (1996). Pathways Downstream of Shc and Grb2 Are Required for Cell Transformation by the Tpr-Met Oncoprotein. J. Biol. Chem. 271: 13116-13122 [Abstract] [Full Text]  
• Guyton, K. Z., Liu, Y., Gorospe, M., Xu, Q., Holbrook, N. J. (1996). Activation of Mitogen-activated Protein Kinase by H(2)O(2). J. Biol. Chem. 271: 4138-4142 [Abstract] [Full Text]  
• Gabbay, R. A., Sutherland, C., Gnudi, L., Kahn, B. B., O'Brien, R. M., Granner, D. K., Flier, J. S. (1996). Insulin Regulation of Phosphoenolpyruvate Carboxykinase Gene Expression Does Not Require Activation of the Ras/Mitogen-activated Protein Kinase Signaling Pathway. J. Biol. Chem. 271: 1890-1897 [Abstract] [Full Text]  
• Urich, M., Shemerly, M. Y. M. E., Besser, D., Nagamine, Y., Ballmer-Hofer, K. (1995). Activation and Nuclear Translocation of Mitogen-activated Protein Kinases by Polyomavirus Middle-T or Serum Depend on Phosphatidylinositol 3-Kinase. J. Biol. Chem. 270: 29286-29292 [Abstract] [Full Text]  
• Harada, S., Smith, R. M., Smith, J. A., Shah, N., Hu, D.-Q., Jarett, L. (1995). Insulin-induced egr-1 Expression in Chinese Hamster Ovary Cells Is Insulin Receptor and Insulin Receptor Substrate-1 Phosphorylation-independent. J. Biol. Chem. 270: 26632-26638 [Abstract] [Full Text]  
• Taha, C., Mitsumoto, Y., Liu, Z., Skolnik, E. Y., Klip, A. (1995). The Insulin-dependent Biosynthesis of GLUT1 and GLUT3 Glucose Transporters in L6 Muscle Cells Is Mediated by Distinct Pathways. J. Biol. Chem. 270: 24678-24681 [Abstract] [Full Text]  
• Cherniack, A. D., Klarlund, J. K., Conway, B. R., Czech, M. P. (1995). Disassembly of Son-of-sevenless Proteins from Grb2 during p21[IMAGE] Desensitization by Insulin. J. Biol. Chem. 270: 1485-1488 [Abstract] [Full Text]  
• Reynet, C, Kahn, C. (1993). Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans. Science 262: 1441-1444 [Abstract]  
• Matter, N., Marx, M., Weg-Remers, S., Ponta, H., Herrlich, P., Konig, H. (2000). Heterogeneous Ribonucleoprotein A1 Is Part of an Exon-specific Splice-silencing Complex Controlled by Oncogenic Signaling Pathways. J. Biol. Chem. 275: 35353-35360 [Abstract] [Full Text]  
• Cuda, G., Paterno, R., Ceravolo, R., Candigliota, M., Perrotti, N., Perticone, F., Faniello, M. C., Schepis, F., Ruocco, A., Mele, E., Cassano, S., Bifulco, M., Santillo, M., Avvedimento, E. V. (2002). Protection of Human Endothelial Cells From Oxidative Stress: Role of Ras-ERK1/2 Signaling. Circulation 105: 968-974 [Abstract] [Full Text]