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Mol Cell Biol. 1991 March; 11(3): 1431-1437

Silencing of the expression of the immunoglobulin kappa gene in non-B cells.

J W Pierce, A M Gifford and D Baltimore

Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.

ABSTRACT

Although the activating factor NF-kappa B can be present in the nucleus of many cell types, transcription and rearrangement of the immunoglobulin kappa chain gene is restricted to cells of the B lineage. Part of this specificity is determined by sequences within the major intron of the kappa gene that specifically silence gene expression in non-B cells (T cells and HeLa cells). These sequences are found in a 232-bp fragment located 5' of the NF-kappa B binding sequence of the enhancer. When this fragment is added back upstream of an active NF-kappa B site, it specifically decreases the expression of a linked gene by more than 10-fold in activated T cells but it has no effect on expression in B cells. The kappa silencer region acts in an orientation- and distance-independent manner and appears to be composed of multiple negative elements. The kappa silencer may act to restrict transcription and rearrangement of the C kappa locus to cells of the B lineage.

Mol Cell Biol. 1991 March; 11(3): 1431-1437




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