This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chen, R H Articles by Blenis, J Search for Related Content PubMed PubMed Citation Articles by Chen, R H Articles by Blenis, J

Regulation of pp90rsk phosphorylation and S6 phosphotransferase activity in Swiss 3T3 cells by growth factor-, phorbol ester-, and cyclic AMP-mediated signal transduction.

Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts 02115.

ABSTRACT

Somatic cell homologs to the Xenopus laevis S6 protein kinases (referred to collectively as pp90rsk) have recently been identified and partially characterized. Here we examine alterations in pp90rsk phosphorylation and S6 phosphotransferase activity in response to regulators of multiple signal transduction systems: purified growth factors, phorbol ester, changes in cyclic AMP (cAMP) levels, and sodium vanadate. All reagents tested increased pp90rsk serine and threonine phosphorylation, but only those agents that regulate cell proliferation and sodium vanadate activated its S6 kinase activity. In addition to the cAMP-stimulated phosphorylation of pp90rsk, a simple correlation between the extent of growth-regulated pp90rsk phosphorylation and S6 phosphotransferase activity was not observed. Quantitative phosphorylation of pp90rsk continued to increase after its S6 kinase activity began its return towards basal levels. However, a close correlation between the appearance and disappearance of a slow-mobility form of phosphorylated pp90rsk (by electrophoresis) and pp90rsk activity was observed. In addition, pp90rsk was regulated by both protein kinase C-independent and -dependent signaling mechanisms. The extent of protein kinase C participation, however, varied depending on which growth factor receptor was activated. Furthermore, growth factor-specific differences in the temporal regulation of pp90rsk S6 phosphotransferase activity were also observed. These results support the notion that the complex regulation of the rsk gene product constitutes one of the primary responses of animal cells to mitogenic signals.

This article has been cited by other articles:

• Itoh, S., Ding, B., Shishido, T., Lerner-Marmarosh, N., Wang, N., Maekawa, N., Berk, B. C., Takeishi, Y., Yan, C., Blaxall, B. C., Abe, J.-i. (2006). Role of p90 Ribosomal S6 Kinase-Mediated Prorenin-Converting Enzyme in Ischemic and Diabetic Myocardium. Circulation 113: 1787-1798 [Abstract] [Full Text]  
• Garton, A. J., Crew, A. P.A., Franklin, M., Cooke, A. R., Wynne, G. M., Castaldo, L., Kahler, J., Winski, S. L., Franks, A., Brown, E. N., Bittner, M. A., Keily, J. F., Briner, P., Hidden, C., Srebernak, M. C., Pirrit, C., O'Connor, M., Chan, A., Vulevic, B., Henninger, D., Hart, K., Sennello, R., Li, A.-H., Zhang, T., Richardson, F., Emerson, D. L., Castelhano, A. L., Arnold, L. D., Gibson, N. W. (2006). OSI-930: A Novel Selective Inhibitor of Kit and Kinase Insert Domain Receptor Tyrosine Kinases with Antitumor Activity in Mouse Xenograft Models. Cancer Res. 66: 1015-1024 [Abstract] [Full Text]  
• Itoh, S., Ding, B., Bains, C. P., Wang, N., Takeishi, Y., Jalili, T., King, G. L., Walsh, R. A., Yan, C., Abe, J.-i. (2005). Role of p90 Ribosomal S6 Kinase (p90RSK) in Reactive Oxygen Species and Protein Kinase C {beta} (PKC-{beta})-mediated Cardiac Troponin I Phosphorylation. J. Biol. Chem. 280: 24135-24142 [Abstract] [Full Text]  
• Zeniou, M., Ding, T., Trivier, E., Hanauer, A. (2002). Expression analysis of RSK gene family members: the RSK2 gene, mutated in Coffin-Lowry syndrome, is prominently expressed in brain structures essential for cognitive function and learning. Hum Mol Genet 11: 2929-2940 [Abstract] [Full Text]  
• Doi, J., Takemori, H., Lin, X.-z., Horike, N., Katoh, Y., Okamoto, M. (2002). Salt-inducible Kinase Represses cAMP-dependent Protein Kinase-mediated Activation of Human Cholesterol Side Chain Cleavage Cytochrome P450 Promoter through the CREB Basic Leucine Zipper Domain. J. Biol. Chem. 277: 15629-15637 [Abstract] [Full Text]  
• Richards, S. A., Dreisbach, V. C., Murphy, L. O., Blenis, J. (2001). Characterization of Regulatory Events Associated with Membrane Targeting of p90 Ribosomal S6 Kinase 1. Mol. Cell. Biol. 21: 7470-7480 [Abstract] [Full Text]  
• Merienne, K., Pannetier, S., Harel-Bellan, A., Sassone-Corsi, P. (2001). Mitogen-Regulated RSK2-CBP Interaction Controls Their Kinase and Acetylase Activities. Mol. Cell. Biol. 21: 7089-7096 [Abstract] [Full Text]  
• Abe, J.-i., Okuda, M., Huang, Q., Yoshizumi, M., Berk, B. C. (2000). Reactive Oxygen Species Activate p90 Ribosomal S6 Kinase via Fyn and Ras. J. Biol. Chem. 275: 1739-1748 [Abstract] [Full Text]  
• Tan, Y., Ruan, H., Demeter, M. R., Comb, M. J. (1999). p90RSK Blocks Bad-mediated Cell Death via a Protein Kinase C-dependent Pathway. J. Biol. Chem. 274: 34859-34867 [Abstract] [Full Text]  
• Trushin, S. A., Pennington, K. N., Algeciras-Schimnich, A., Paya, C. V. (1999). Protein Kinase C and Calcineurin Synergize to Activate Ikappa B Kinase and NF-kappa B in T Lymphocytes. J. Biol. Chem. 274: 22923-22931 [Abstract] [Full Text]  
• Li, H.-L., Davis, W., Pure, E. (1999). Suboptimal Cross-linking of Antigen Receptor Induces Syk-dependent Activation of p70S6 Kinase through Protein Kinase C and Phosphoinositol 3-Kinase. J. Biol. Chem. 274: 9812-9820 [Abstract] [Full Text]  
• Markuns, J. F., Napoli, R., Hirshman, M. F., Davalli, A. M., Cheatham, B., Goodyear, L. J. (1999). Effects of Streptozocin-Induced Diabetes and Islet Cell Transplantation on Insulin Signaling in Rat Skeletal Muscle. Endocrinology 140: 106-111 [Abstract] [Full Text]  
• Angenstein, F., Greenough, W. T., Weiler, I. J. (1998). Metabotropic glutamate receptor-initiated translocation of protein kinase p90rsk to polyribosomes: A possible factor regulating synaptic protein synthesis. Proc. Natl. Acad. Sci. USA 95: 15078-15083 [Abstract] [Full Text]  
• De Cesare, D., Jacquot, S., Hanauer, A., Sassone-Corsi, P. (1998). Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene. Proc. Natl. Acad. Sci. USA 95: 12202-12207 [Abstract] [Full Text]  
• Joel, P. B., Smith, J., Sturgill, T. W., Fisher, T. L., Blenis, J., Lannigan, D. A. (1998). pp90rsk1 Regulates Estrogen Receptor-Mediated Transcription through Phosphorylation of Ser-167. Mol. Cell. Biol. 18: 1978-1984 [Abstract] [Full Text]  
• Takahashi, E., Abe, J.-i., Berk, B. C. (1997). Angiotensin II Stimulates p90rsk in Vascular Smooth Muscle Cells : A Potential Na+-H+ Exchanger Kinase. Circ. Res. 81: 268-273 [Abstract] [Full Text]  
• Li, H.-L., Forman, M. S., Kurosaki, T., Pure, E. (1997). Syk Is Required for BCR-mediated Activation of p90Rsk, but Not p70S6k, via a Mitogen-activated Protein Kinase-independent Pathway in B Cells. J. Biol. Chem. 272: 18200-18208 [Abstract] [Full Text]  
• Zhao, Y., Bjorbak, C., Moller, D. E. (1996). Regulation and Interaction of pp90rsk Isoforms with Mitogen-activated Protein Kinases. J. Biol. Chem. 271: 29773-29779 [Abstract] [Full Text]  
• Petritsch, C., Woscholski, Rüd., Edelmann, H. M. L., Ballou, L. M. (1995). Activation of p70 S6 Kinase and erk-encoded Mitogen-activated Protein Kinases Is Resistant to High Cyclic Nucleotide Levels in Swiss 3T3 Fibroblasts. J. Biol. Chem. 270: 26619-26625 [Abstract] [Full Text]  
• Han, J.-W., Pearson, R. B., Dennis, P. B., Thomas, G. (1995). Rapamycin, Wortmannin, and the Methylxanthine SQ20006 Inactivate p70[IMAGE] by Inducing Dephosphorylation of the Same Subset of Sites. J. Biol. Chem. 270: 21396-21403 [Abstract] [Full Text]  
• Bjørbæk, C., Zhao, Y., Moller, D. E. (1995). Divergent Functional Roles for p90[IMAGE] Kinase Domains. J. Biol. Chem. 270: 18848-18852 [Abstract] [Full Text]  
• Zhang, Y., Zhong, S., Dong, Z., Chen, N., Bode, A. M., Ma, W.-y., Dong, Z. (2001). UVA Induces Ser381 Phosphorylation of p90RSK/MAPKAP-K1 via ERK and JNK Pathways. J. Biol. Chem. 276: 14572-14580 [Abstract] [Full Text]