This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Friesel, R Articles by Dawid, I B Search for Related Content PubMed PubMed Citation Articles by Friesel, R Articles by Dawid, I B

cDNA cloning and developmental expression of fibroblast growth factor receptors from Xenopus laevis.

Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.

ABSTRACT

The heparin-binding growth factors constitute a family of homologous polypeptides including basic and acidic fibroblast growth factors (FGFs). These factors participate in a variety of processes, including wound healing, angiogenesis, neuronal survival, and inductive events in the early amphibian embryo. We have isolated three closely related species of cDNA clones for Xenopus FGF receptors. One of these, designated XFGFR-A1, encodes an open reading frame of 814 amino acids. A second class encodes an identical amino acid sequence with the exception of an 88-amino-acid deletion near the 5' end. This species probably arises through alternative splicing. A third class of cDNA corresponding to the shorter form of XFGFR-A1 was isolated and shown to be 95% homologous and is designated XFGFR-A2. Xenopus FGF receptors are similar to FGF receptors from other species in that they contain a transmembrane domain, a tyrosine kinase domain split by a 14-amino-acid insertion, and a unique conserved stretch of eight acidic residues in the extracellular domain. Overexpression of Xenopus FGF receptor protein by transfection of COS1 cells with the corresponding cDNA in a transient expression vector leads to the appearance of new FGF binding sites on transfected cells, consistent with these cDNAs encoding for FGF receptors. RNA gel blot analysis demonstrates that Xenopus FGF receptor mRNA is a maternal message and is expressed throughout early development. When blastula-stage ectoderm is cultured in control amphibian salt solutions, Xenopus FGF receptor mRNA declines to undetectable levels by late neurula stages. However, when cultured in the presence of FGF of XTC mesoderm-inducing factor, Xenopus FGF receptor RNA expression is maintained.

This article has been cited by other articles:

• Monsoro-Burq, A.-H., Fletcher, R. B., Harland, R. M. (2003). Neural crest induction by paraxial mesoderm in Xenopus embryos requires FGF signals. Development 130: 3111-3124 [Abstract] [Full Text]  
• Carron, C., Pascal, A., Djiane, A., Boucaut, J.-C., Shi, D.-L., Umbhauer, M. (2003). Frizzled receptor dimerization is sufficient to activate the Wnt/{beta}-catenin pathway. J. Cell Sci. 116: 2541-2550 [Abstract] [Full Text]  
• Paterno, G. D., Ryan, P. J., Kao, K. R., Gillespie, L. L. (2000). The VT+ and VT- Isoforms of the Fibroblast Growth Factor Receptor Type 1 Are Differentially Expressed in the Presumptive Mesoderm of Xenopus Embryos and Differ in Their Ability to Mediate Mesoderm Formation. J. Biol. Chem. 275: 9581-9586 [Abstract] [Full Text]  
• Umbhauer, M, Penzo-Mendez, A, Clavilier, L, Boucaut, J, Riou, J (2000). Signaling specificities of fibroblast growth factor receptors in early Xenopus embryo. J. Cell Sci. 113: 2865-2875 [Abstract]  
• LaVallee, T. M., Prudovsky, I. A., McMahon, G. A., Hu, X., Maciag, T. (1998). Activation of the MAP Kinase Pathway by FGF-1 Correlates with Cell Proliferation Induction While Activation of the Src Pathway Correlates with Migration. J. Cell Biol. 141: 1647-1658 [Abstract] [Full Text]  
• TAKAHASHI, K., OKAMURA, Y. (1998). Ion Channels and Early Development of Neural Cells. Physiol. Rev. 78: 307-337 [Abstract] [Full Text]  
• McFarlane, S, Zuber, M., Holt, C. (1998). A role for the fibroblast growth factor receptor in cell fate decisions in the developing vertebrate retina. Development 125: 3967-3975 [Abstract]  
• Walz, A, McFarlane, S, Brickman, Y., Nurcombe, V, Bartlett, P., Holt, C. (1997). Essential role of heparan sulfates in axon navigation and targeting in the developing visual system. Development 124: 2421-2430 [Abstract]  
• Neilson, K. M., Friesel, R. (1996). Ligand-independent Activation of Fibroblast Growth Factor Receptors by Point Mutations in the Extracellular, Transmembrane, and Kinase Domains. J. Biol. Chem. 271: 25049-25057 [Abstract] [Full Text]  
• Prudovsky, I. A., Savion, N., LaVallee, T. M., Maciag, T. (1996). The Nuclear Trafficking of Extracellular Fibroblast Growth Factor (FGF)-1 Correlates with the Perinuclear Association of the FGF Receptor-1alpha Isoforms but Not the FGF Receptor-1beta Isoforms. J. Biol. Chem. 271: 14198-14205 [Abstract] [Full Text]  
• Neilson, K. M., Friesel, R. E. (1995). Constitutive Activation of Fibroblast Growth Factor Receptor-2 by a Point Mutation Associated with Crouzon Syndrome. J. Biol. Chem. 270: 26037-26040 [Abstract] [Full Text]  
• Gillespie, L. L., Chen, G., Paterno, G. D. (1995). Cloning of a Fibroblast Growth Factor Receptor 1 Splice Variant from Xenopus Embryos That Lacks a Protein Kinase C Site Important for the Regulation of Receptor Activity. J. Biol. Chem. 270: 22758-22763 [Abstract] [Full Text]  
• Mathieu, M., Kiefer, P., Mason, I., Dickson, C. (1995). Fibroblast Growth Factor (FGF) 3 from Xenopus laevis (XFGF3) Binds with High Affinity to FGF Receptor 2. J. Biol. Chem. 270: 6779-6787 [Abstract] [Full Text]  
• Kengaku, M, Okamoto, H (1995). bFGF as a possible morphogen for the anteroposterior axis of the central nervous system in Xenopus. Development 121: 3121-3130 [Abstract]  
• Robbie, E., Peterson, M, Amaya, E, Musci, T. (1995). Temporal regulation of the Xenopus FGF receptor in development: a translation inhibitory element in the 3' untranslated region. Development 121: 1775-1785 [Abstract]  
• Kessler, D., Melton, D. (1994). Vertebrate embryonic induction: mesodermal and neural patterning. Science 266: 596-604 [Abstract]  
• Shi, D., Fromentoux, V, Launay, C, Umbhauer, M, Boucaut, J. (1994). Isolation and developmental expression of the amphibian homolog of the fibroblast growth factor receptor 3. J. Cell Sci. 107: 417-425 [Abstract]  
• Marcelle, C, Eichmann, A, Halevy, O, Breant, C, Le Douarin, N. (1994). Distinct developmental expression of a new avian fibroblast growth factor receptor. Development 120: 683-694 [Abstract]  
• Kengaku, M., Okamoto, H. (1993). Basic fibroblast growth factor induces differentiation of neural tube and neural crest lineages of cultured ectoderm cells from Xenopus gastrula. Development 119: 1067-1078 [Abstract]  
• Shishido, E, Higashijima, S, Emori, Y, Saigo, K (1993). Two FGF-receptor homologues of Drosophila: one is expressed in mesodermal primordium in early embryos. Development 117: 751-761 [Abstract]  
• Friesel, R., Brown, S. A. (1992). Spatially restricted expression of fibroblast growth factor receptor-2 during Xenopus development. Development 116: 1051-1058 [Abstract]  
• Kimelman, D., Christian, J. L., Moon, R. T. (1992). Synergistic principles of development: overlapping patterning systems in Xenopus mesoderm induction. Development 116: 1-9 [Abstract]  
• Shi, D., Feige, J., Riou, J., DeSimone, D., Boucaut, J. (1992). Differential expression and regulation of two distinct fibroblast growth factor receptors during early development of the urodele amphibian Pleurodeles waltl. Development 116: 261-273 [Abstract]