This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Smith, B J Articles by Yaffe, M P Search for Related Content PubMed PubMed Citation Articles by Smith, B J Articles by Yaffe, M P

 Previous Article  |  Next Article 

Mol Cell Biol. 1991 May; 11(5): 2647-2655

A mutation in the yeast heat-shock factor gene causes temperature-sensitive defects in both mitochondrial protein import and the cell cycle.

Department of Biology, University of California, San Diego, La Jolla 92093.

ABSTRACT

Yeast cells containing the recessive mas3 mutation display temperature-sensitive defects in both mitochondrial protein import and the cell division cycle. The import defect is characterized by two pools of mitochondrial precursors and a dramatically slower rate of posttranslational import. The effect of mas3 on cell cycle progression occurs within one cell cycle at the nonpermissive temperature and retards progression through the G2 stage. The mas3 mutation maps to the gene encoding yeast heat-shock transcription factor (HSF), and expression of wild-type HSF complements the temperature-sensitive defects. The mas3 lesion has no apparent effect on protein secretion. In mas3 cells, induction of a major heat-shock gene, SSA1, is defective at 37 degrees C. The properties of the mas3 mutant cells indicate that HSF mediates the response to stress of two basic cellular processes: mitochondrial protein import and cell cycle progression.

This article has been cited by other articles:

• Shahi, P., Gulshan, K., Moye-Rowley, W. S. (2007). Negative Transcriptional Regulation of Multidrug Resistance Gene Expression by an Hsp70 Protein. J. Biol. Chem. 282: 26822-26831 [Abstract] [Full Text]  
• Yamamoto, A., Mizukami, Y., Sakurai, H. (2005). Identification of a Novel Class of Target Genes and a Novel Type of Binding Sequence of Heat Shock Transcription Factor in Saccharomyces cerevisiae. J. Biol. Chem. 280: 11911-11919 [Abstract] [Full Text]  
• Weir, B. A., Yaffe, M. P. (2004). Mmd1p, a Novel, Conserved Protein Essential for Normal Mitochondrial Morphology and Distribution in the Fission Yeast Schizosaccharomyces pombe. Mol. Biol. Cell 15: 1656-1665 [Abstract] [Full Text]  
• Davies, B. A., Topp, J. D., Sfeir, A. J., Katzmann, D. J., Carney, D. S., Tall, G. G., Friedberg, A. S., Deng, L., Chen, Z., Horazdovsky, B. F. (2003). Vps9p CUE Domain Ubiquitin Binding Is Required for Efficient Endocytic Protein Traffic. J. Biol. Chem. 278: 19826-19833 [Abstract] [Full Text]  
• Cronin, S. R., Rao, R., Hampton, R. Y. (2002). Cod1p/Spf1p is a P-type ATPase involved in ER function and Ca2+ homeostasis. J. Cell Biol. 157: 1017-1028 [Abstract] [Full Text]  
• Nanduri, J., Tartakoff, A. M. (2001). Perturbation of the Nucleus: A Novel Hog1p-independent, Pkc1p-dependent Consequence of Hypertonic Shock in Yeast. Mol. Biol. Cell 12: 1835-1841 [Abstract] [Full Text]  
• Fekkes, P., Shepard, K. A., Yaffe, M. P. (2000). Gag3p, an Outer Membrane Protein Required for Fission of Mitochondrial Tubules. J. Cell Biol. 151: 333-340 [Abstract] [Full Text]  
• Raitt, D. C., Johnson, A. L., Erkine, A. M., Makino, K., Morgan, B., Gross, D. S., Johnston, L. H. (2000). The Skn7 Response Regulator of Saccharomyces cerevisiae Interacts with Hsf1 In Vivo and Is Required for the Induction of Heat Shock Genes by Oxidative Stress. Mol. Biol. Cell 11: 2335-2347 [Abstract] [Full Text]  
• Liu, X.-D., Morano, K. A., Thiele, D. J. (1999). The Yeast Hsp110 Family Member, Sse1, Is an Hsp90 Cochaperone. J. Biol. Chem. 274: 26654-26660 [Abstract] [Full Text]  
• Fisk, H. A., Yaffe, M. P. (1999). A Role for Ubiquitination in Mitochondrial Inheritance in Saccharomyces cerevisiae. J. Cell Biol. 145: 1199-1208 [Abstract] [Full Text]  
• Hong, Y., Sarge, K. D. (1999). Regulation of Protein Phosphatase 2A Activity by Heat Shock Transcription Factor 2. J. Biol. Chem. 274: 12967-12970 [Abstract] [Full Text]  
• Shepard, K. A., Yaffe, M. P. (1999). The Yeast Dynamin-like Protein, Mgm1p, Functions on the Mitochondrial Outer Membrane to Mediate Mitochondrial Inheritance. J. Cell Biol. 144: 711-720 [Abstract] [Full Text]  
• Morano, K. A., Santoro, N., Koch, K. A., Thiele, D. J. (1999). A trans-Activation Domain in Yeast Heat Shock Transcription Factor Is Essential for Cell Cycle Progression during Stress. Mol. Cell. Biol. 19: 402-411 [Abstract] [Full Text]  
• Treger, J. M., Schmitt, A. P., Simon, J. R., McEntee, K. (1998). Transcriptional Factor Mutations Reveal Regulatory Complexities of Heat Shock and Newly Identified Stress Genes in Saccharomyces cerevisiae. J. Biol. Chem. 273: 26875-26879 [Abstract] [Full Text]  
• Berger, K. H., Yaffe, M. P. (1998). Prohibitin Family Members Interact Genetically with Mitochondrial Inheritance Components in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 4043-4052 [Abstract] [Full Text]  
• Fisk, H. A., Yaffe, M. P. (1997). Mutational Analysis of Mdm1p Function in Nuclear and Mitochondrial Inheritance. J. Cell Biol. 138: 485-494 [Abstract] [Full Text]  
• Berger, K. H., Sogo, L. F., Yaffe, M. P. (1997). Mdm12p, a Component Required for Mitochondrial Inheritance That Is Conserved between Budding and Fission Yeast. J. Cell Biol. 136: 545-553 [Abstract] [Full Text]  
• Zarzov, P, Boucherie, H, Mann, C (1997). A yeast heat shock transcription factor (Hsf1) mutant is defective in both Hsc82/Hsp82 synthesis and spindle pole body duplication. J. Cell Sci. 110: 1879-1891 [Abstract]  
• Zhu, D, Dix, D., Eddy, E. (1997). HSP70-2 is required for CDC2 kinase activity in meiosis I of mouse spermatocytes. Development 124: 3007-3014 [Abstract]  
• Kaplon, T., Jacquet, M. (1995). The Cellular Content of Cdc25p, the Ras Exchange Factor in Saccharomyces cerevisiae, Is Regulated by Destabilization Through a Cyclin Destruction Box. J. Biol. Chem. 270: 20742-20747 [Abstract] [Full Text]  
• Traven, A., Wong, J. M. S., Xu, D., Sopta, M., Ingles, C. J. (2001). Interorganellar Communication. ALTERED NUCLEAR GENE EXPRESSION PROFILES IN A YEAST MITOCHONDRIAL DNA MUTANT. J. Biol. Chem. 276: 4020-4027 [Abstract] [Full Text]