This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mechti, N
Right arrow Articles by Lebleu, B
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mechti, N
Right arrow Articles by Lebleu, B

 Previous Article  |  Next Article 

Mol Cell Biol. 1991 May; 11(5): 2832-2841

Sequence requirements for premature transcription arrest within the first intron of the mouse c-fos gene.

N Mechti, M Piechaczyk, J M Blanchard, P Jeanteur and B Lebleu

Laboratoire de Biochimie des Protéines, URA CNRS 1191, Génétique Moléculaire, Université des Sciences et Techniques du Languedoc, Montpellier, France.

ABSTRACT

A strong block to the elongation of nascent RNA transcripts by RNA polymerase II occurs in the 5' part of the mammalian c-fos proto-oncogene. In addition to the control of initiation, this mechanism contributes to transcriptional regulation of the gene. In vitro transcription experiments using nuclear extracts and purified transcription templates allowed us to map a unique arrest site within the mouse first intron 385 nucleotides downstream from the promoter. This position is in keeping with that estimated from nuclear run-on assays performed with short DNA probes and thus suggests that it corresponds to the actual block in vivo. Moreover, we have shown that neither the c-fos promoter nor upstream sequences are absolute requirements for an efficient transcription arrest both in vivo and in vitro. Finally, we have characterized a 103-nucleotide-long intron 1 motif comprising the arrest site and sufficient for obtaining the block in a cell-free transcription assay.

Mol Cell Biol. 1991 May; 11(5): 2832-2841




This article has been cited by other articles:

  • Ryser, S., Fujita, T., Tortola, S., Piuz, I., Schlegel, W. (2007). The Rate of c-fos Transcription in Vivo Is Continuously Regulated at the Level of Elongation by Dynamic Stimulus-coupled Recruitment of Positive Transcription Elongation Factor b. J. Biol. Chem. 282: 5075-5084 [Abstract] [Full Text]  
  • Cha-Molstad, H., Keller, D. M., Yochum, G. S., Impey, S., Goodman, R. H. (2004). Cell-type-specific binding of the transcription factor CREB to the cAMP-response element. Proc. Natl. Acad. Sci. USA 101: 13572-13577 [Abstract] [Full Text]  
  • Blanchette, M., Tompa, M. (2002). Discovery of Regulatory Elements by a Computational Method for Phylogenetic Footprinting. Genome Res 12: 739-748 [Abstract] [Full Text]  
  • Gongora, C., Degols, G., Espert, L., Hua, T. D., Mechti, N. (2000). A unique ISRE, in the TATA-less human Isg20 promoter, confers IRF-1-mediated responsiveness to both interferon type I and type II. Nucleic Acids Res 28: 2333-2341 [Abstract] [Full Text]  
  • SUSINI, S., VAN HAASTEREN, G., LI, S., PRENTKI, M., SCHLEGEL, W. (2000). Essentiality of intron control in the induction of c-fos by glucose and glucoincretin peptides in INS-1 {beta}-cells. FASEB J. 14: 128-136 [Abstract] [Full Text]  
  • Coulon, V., Veyrune, J.-L., Tourkine, N., Vie, A., Hipskind, R. A., Blanchard, J.-M. (1999). A Novel Calcium Signaling Pathway Targets the c-fos Intragenic Transcriptional Pausing Site. J. Biol. Chem. 274: 30439-30446 [Abstract] [Full Text]  
  • Dallinger, G., Oberkofler, H., Seelos, C., Patsch, W. (1999). Transcriptional elongation of the rat apolipoprotein A-I gene: identification and mapping of two arrest sites and their signals. J. Lipid Res. 40: 1229-1239 [Abstract] [Full Text]  
  • Kumahara, E., Ebihara, T., Saffen, D. (1999). Protein Kinase Inhibitor H7 Blocks the Induction of Immediate-Early Genes zif268 and c-fos by a Mechanism Unrelated to Inhibition of Protein Kinase C but Possibly Related to Inhibition of Phosphorylation of RNA Polymerase II. J. Biol. Chem. 274: 10430-10438 [Abstract] [Full Text]  
  • Gongora, C., David, G., Pintard, L., Tissot, C., Hua, T. D., Dejean, A., Mechti, N. (1997). Molecular Cloning of a New Interferon-induced PML Nuclear Body-associated Protein. J. Biol. Chem. 272: 19457-19463 [Abstract] [Full Text]  
  • Marshall, N. F., Price, D. H. (1995). Purification of P-TEFb, a Transcription Factor Required for the Transition into Productive Elongation. J. Biol. Chem. 270: 12335-12338 [Abstract] [Full Text]  
  • Thompson, M. A., Ginty, D. D., Bonni, A., Greenberg, M. E. (1995). L-type Voltage-sensitive Ca[IMAGE] Channel Activation Regulates c-fos Transcription at Multiple Levels. J. Biol. Chem. 270: 4224-4235 [Abstract] [Full Text]  
  • Kan, J. L. C., Moran, R. G. (1995). Analysis of a Mouse Gene Encoding Three Steps of Purine Synthesis Reveals Use of an Intronic Polyadenylation Signal without Alternative Exon Usage. J. Biol. Chem. 270: 1823-1832 [Abstract] [Full Text]  
  • Carroll, J. M., Taichman, L. B. (1992). Characterization of the human involucrin promoter using a transient beta-galactosidase assay. J. Cell Sci. 103: 925-930 [Abstract]  
  • Morrow, M A, Lee, G, Gillis, S, Yancopoulos, G D, Alt, F W (1992). Interleukin-7 induces N-myc and c-myc expression in normal precursor B lymphocytes.. Genes Dev. 6: 61-70 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»