This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martinez, E Articles by Mermod, N Search for Related Content PubMed PubMed Citation Articles by Martinez, E Articles by Mermod, N

Synergistic transcriptional activation by CTF/NF-I and the estrogen receptor involves stabilized interactions with a limiting target factor.

Institut de Biologie Animale, Bâtiment de Biologie, Université de Lausanne, Switzerland.

ABSTRACT

Transcription initiation at eukaryotic protein-coding gene promoters is regulated by a complex interplay of site-specific DNA-binding proteins acting synergistically or antagonistically. Here, we have analyzed the mechanisms of synergistic transcriptional activation between members of the CCAAT-binding transcription factor/nuclear factor I (CTF/NF-I) family and the estrogen receptor. By using cotransfection experiments with HeLa cells, we show that the proline-rich transcriptional activation domain of CTF-1, when fused to the GAL4 DNA-binding domain, synergizes with each of the two estrogen receptor-activating regions. Cooperative DNA binding between the GAL4-CTF-1 fusion and the estrogen receptor does not occur in vitro, and in vivo competition experiments demonstrate that both activators can be specifically inhibited by the overexpression of a proline-rich competitor, indicating that a common limiting factor is mediating their transcriptional activation functions. Furthermore, the two activators functioning synergistically are much more resistant to competition than either factor alone, suggesting that synergism between CTF-1 and the estrogen receptor is the result of a stronger tethering of the limiting target factor(s) to the two promoter-bound activators.

This article has been cited by other articles:

• LaRochelle, O., Labbe, S., Harrisson, J.-F., Simard, C., Tremblay, V., St-Gelais, G., Govindan, M. V., Seguin, C. (2008). Nuclear Factor-1 and Metal Transcription Factor-1 Synergistically Activate the Mouse Metallothionein-1 Gene in Response to Metal Ions. J. Biol. Chem. 283: 8190-8201 [Abstract] [Full Text]  
• Emi, Y., Ueda, K., Ohnishi, A., Ikushiro, S.-i., Iyanagi, T. (2005). Transcriptional Enhancement of UDP-Glucuronosyltransferase Form 1A2 (UGT1A2) by Nuclear Factor I-A (NFI-A) in Rat Hepatocytes. J Biochem 138: 313-325 [Abstract] [Full Text]  
• Bonofiglio, D., Gabriele, S., Aquila, S., Catalano, S., Gentile, M., Middea, E., Giordano, F., Ando, S. (2005). Estrogen Receptor {alpha} Binds to Peroxisome Proliferator-Activated Receptor Response Element and Negatively Interferes with Peroxisome Proliferator-Activated Receptor {gamma} Signaling in Breast Cancer Cells. Clin. Cancer Res. 11: 6139-6147 [Abstract] [Full Text]  
• Ferrari, S., Simmen, K. C., Dusserre, Y., Muller, K., Fourel, G., Gilson, E., Mermod, N. (2004). Chromatin Domain Boundaries Delimited by a Histone-binding Protein in Yeast. J. Biol. Chem. 279: 55520-55530 [Abstract] [Full Text]  
• Qin, C., Nguyen, T., Stewart, J., Samudio, I., Burghardt, R., Safe, S. (2002). Estrogen Up-Regulation of p53 Gene Expression in MCF-7 Breast Cancer Cells Is Mediated by Calmodulin Kinase IV-Dependent Activation of a Nuclear Factor {kappa}B/CCAAT-Binding Transcription Factor-1 Complex. Mol. Endocrinol. 16: 1793-1809 [Abstract] [Full Text]  
• Morel, Y., Coumoul, X., Nalpas, A., Barouki, R. (2001). Nuclear Factor I/CCAAT Box Transcription Factor trans-Activating Domain Is a Negative Sensor of Cellular Stress. Mol. Pharmacol. 58: 1239-1246 [Abstract] [Full Text]  
• Muller, K., Mermod, N. (2000). The Histone-interacting Domain of Nuclear Factor I Activates Simian Virus 40 DNA Replication in Vivo. J. Biol. Chem. 275: 1645-1650 [Abstract] [Full Text]  
• Cook, T., Gebelein, B., Belal, M., Mesa, K., Urrutia, R. (1999). Three Conserved Transcriptional Repressor Domains Are a Defining Feature of the TIEG Subfamily of Sp1-like Zinc Finger Proteins. J. Biol. Chem. 274: 29500-29504 [Abstract] [Full Text]  
• Chaudhry, A. Z., Vitullo, A. D., Gronostajski, R. M. (1999). Nuclear Factor I-mediated Repression of the Mouse Mammary Tumor Virus Promoter Is Abrogated by the Coactivators p300/CBP and SRC-1. J. Biol. Chem. 274: 7072-7081 [Abstract] [Full Text]  
• Ricci, M. S., Toscano, D. G., Mattingly, C. J., Toscano, W. A. Jr. (1999). Estrogen Receptor Reduces CYP1A1 Induction in Cultured Human Endometrial Cells. J. Biol. Chem. 274: 3430-3438 [Abstract] [Full Text]  
• Tsai-Pflugfelder, M., Gasser, S. M., Wahli, W. (1998). Functional Interaction between the Estrogen Receptor and CTF1: Analysis of the Vitellogenin Gene B1 Promoter in Yeast. Mol. Endocrinol. 12: 1525-1541 [Abstract] [Full Text]  
• Alevizopoulos, A., Dusserre, Y., Ruegg, U., Mermod, N. (1997). Regulation of the Transforming Growth Factor beta -responsive Transcription Factor CTF-1 by Calcineurin and Calcium/ Calmodulin-dependent Protein Kinase IV. J. Biol. Chem. 272: 23597-23605 [Abstract] [Full Text]  
• de Medeiros, S. R. B., Krey, G., Hihi, A. K., Wahli, W. (1997). Functional Interactions between the Estrogen Receptor and the Transcription Activator Sp1 Regulate the Estrogen-dependent Transcriptional Activity of the Vitellogenin A1 io Promoter. J. Biol. Chem. 272: 18250-18260 [Abstract] [Full Text]  
• Alevizopoulos, A., Mermod, N. (1996). Antagonistic Regulation of a Proline-rich Transcription Factor by Transforming Growth Factor beta and Tumor Necrosis Factor alpha. J. Biol. Chem. 271: 29672-29681 [Abstract] [Full Text]  
• Harnish, D. C., Malik, S., Kilbourne, E., Costa, R., Karathanasis, S. K. (1996). Control of Apolipoprotein AI Gene Expression through Synergistic Interactions between Hepatocyte Nuclear Factors 3and 4. J. Biol. Chem. 271: 13621-13628 [Abstract] [Full Text]  
• Alevizopoulos, A, Dusserre, Y, Tsai-Pflugfelder, M, von der Weid, T, Wahli, W, Mermod, N (1995). A proline-rich TGF-beta-responsive transcriptional activator interacts with histone H3.. Genes Dev. 9: 3051-3066 [Abstract]  
• Holloway, J M, Szeto, D P, Scully, K M, Glass, C K, Rosenfeld, M G (1995). Pit-1 binding to specific DNA sites as a monomer or dimer determines gene-specific use of a tyrosine-dependent synergy domain.. Genes Dev. 9: 1992-2006 [Abstract]  
• Tansey, W P, Ruppert, S, Tjian, R, Herr, W (1994). Multiple regions of TBP participate in the response to transcriptional activators in vivo.. Genes Dev. 8: 2756-2769 [Abstract]  
• Halachmi, S, Marden, E, Martin, G, MacKay, H, Abbondanza, C, Brown, M (1994). Estrogen receptor-associated proteins: possible mediators of hormone-induced transcription. Science 264: 1455-1458 [Abstract]  
• Robyr, D., Gegonne, A., Wolffe, A. P., Wahli, W. (2000). Determinants of Vitellogenin B1 Promoter Architecture. HNF3 AND ESTROGEN RESPONSIVE TRANSCRIPTION WITHIN CHROMATIN. J. Biol. Chem. 275: 28291-28300 [Abstract] [Full Text]