This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hazel, T G Articles by Lau, L F Search for Related Content PubMed PubMed Citation Articles by Hazel, T G Articles by Lau, L F

 Previous Article  |  Next Article 

Mol Cell Biol. 1991 June; 11(6): 3239-3246

Nur77 is differentially modified in PC12 cells upon membrane depolarization and growth factor treatment.

Department of Genetics, University of Illinois College of Medicine, Chicago 60612.

ABSTRACT

The rat pheochromocytoma cell line PC12 can be induced by growth factors to undergo proliferation and neuronal differentiation. These cells also have excitable membranes that can be depolarized by neurotransmitters or elevated levels of extracellular KCl. Treatment of PC12 cells with growth factors or membrane-depolarizing agents rapidly activates the expression of specific genes whose products are thought to mediate the subsequent biological responses. One such gene, nur77, is a member of the steroid and thyroid hormone receptor gene superfamily. We have identified the Nur77 protein and shown that it is synthesized rapidly and transiently in PC12 cells following stimulation, has a short half-life of 30 to 40 min, and is located in both the nucleus and the cytoplasm. Nur77 is posttranslationally modified, primarily by phosphorylation on serine residues. Phosphopeptide analysis reveals that Nur77 is modified differently upon membrane depolarization than after treatment with growth factors. We hypothesize that the activity of Nur77 is regulated by both differential gene expression and posttranslational modification and that these modes of regulation contribute to distinct downstream responses specific to membrane depolarization and growth factor treatment.

This article has been cited by other articles:

• Cunningham, N. R., Artim, S. C., Fornadel, C. M., Sellars, M. C., Edmonson, S. G., Scott, G., Albino, F., Mathur, A., Punt, J. A. (2006). Immature CD4+CD8+ Thymocytes and Mature T Cells Regulate Nur77 Distinctly in Response to TCR Stimulation. J. Immunol. 177: 6660-6666 [Abstract] [Full Text]  
• Kanzleiter, T., Schneider, T., Walter, I., Bolze, F., Eickhorst, C., Heldmaier, G., Klaus, S., Klingenspor, M. (2005). Evidence for Nr4a1 as a cold-induced effector of brown fat thermogenesis. Physiol. Genomics 24: 37-44 [Abstract] [Full Text]  
• Havelock, J. C., Smith, A. L., Seely, J. B., Dooley, C. A., Rodgers, R. J., Rainey, W. E., Carr, B. R. (2005). The NGFI-B family of transcription factors regulates expression of 3{beta}-hydroxysteroid dehydrogenase type 2 in the human ovary. Mol Hum Reprod 11: 79-85 [Abstract] [Full Text]  
• Jacobs, C. M., Boldingh, K. A., Slagsvold, H. H., Thoresen, G. H., Paulsen, R. E. (2004). ERK2 Prohibits Apoptosis-induced Subcellular Translocation of Orphan Nuclear Receptor NGFI-B/TR3. J. Biol. Chem. 279: 50097-50101 [Abstract] [Full Text]  
• Cao, X., Liu, W., Lin, F., Li, H., Kolluri, S. K., Lin, B., Han, Y.-h., Dawson, M. I., Zhang, X.-k. (2004). Retinoid X Receptor Regulates Nur77/Thyroid Hormone Receptor 3-Dependent Apoptosis by Modulating Its Nuclear Export and Mitochondrial Targeting. Mol. Cell. Biol. 24: 9705-9725 [Abstract] [Full Text]  
• Song, K.-H., Park, Y.-Y., Park, K. C., Hong, C. Y., Park, J. H., Shong, M., Lee, K., Choi, H.-S. (2004). The Atypical Orphan Nuclear Receptor DAX-1 Interacts with Orphan Nuclear Receptor Nur77 and Represses Its Transactivation. Mol. Endocrinol. 18: 1929-1940 [Abstract] [Full Text]  
• Kolluri, S. K., Bruey-Sedano, N., Cao, X., Lin, B., Lin, F., Han, Y.-H., Dawson, M. I., Zhang, X.-k. (2003). Mitogenic Effect of Orphan Receptor TR3 and Its Regulation by MEKK1 in Lung Cancer Cells. Mol. Cell. Biol. 23: 8651-8667 [Abstract] [Full Text]  
• Wansa, K. D. S. A., Harris, J. M., Yan, G., Ordentlich, P., Muscat, G. E. O. (2003). The AF-1 Domain of the Orphan Nuclear Receptor NOR-1 Mediates Trans-activation, Coactivator Recruitment, and Activation by the Purine Anti-metabolite 6-Mercaptopurine. J. Biol. Chem. 278: 24776-24790 [Abstract] [Full Text]  
• Maira, M., Martens, C., Batsche, E., Gauthier, Y., Drouin, J. (2003). Dimer-Specific Potentiation of NGFI-B (Nur77) Transcriptional Activity by the Protein Kinase A Pathway and AF-1-Dependent Coactivator Recruitment. Mol. Cell. Biol. 23: 763-776 [Abstract] [Full Text]  
• Wansa, K. D. S. A., Harris, J. M., Muscat, G. E. O. (2002). The Activation Function-1 Domain of Nur77/NR4A1 Mediates Trans-activation, Cell Specificity, and Coactivator Recruitment. J. Biol. Chem. 277: 33001-33011 [Abstract] [Full Text]  
• Song, K.-H., Lee, K., Choi, H.-S. (2002). Endocrine Disrupter Bisphenol A Induces Orphan Nuclear Receptor Nur77 Gene Expression and Steroidogenesis in Mouse Testicular Leydig Cells. Endocrinology 143: 2208-2215 [Abstract] [Full Text]  
• Song, K.-H., Park, J.-I., Lee, M.-O., Soh, J., Lee, K., Choi, H.-S. (2001). LH Induces Orphan Nuclear Receptor Nur77 Gene Expression in Testicular Leydig Cells. Endocrinology 142: 5116-5123 [Abstract] [Full Text]  
• Park, J.-I., Park, H.-J., Choi, H.-S., Lee, K., Lee, W.-K., Chun, S.-Y. (2001). Gonadotropin Regulation of NGFI-B Messenger Ribonucleic Acid Expression during Ovarian Follicle Development in the Rat. Endocrinology 142: 3051-3059 [Abstract] [Full Text]  
• Giguère, V. (1999). Orphan Nuclear Receptors: From Gene to Function. Endocr. Rev. 20: 689-725 [Abstract] [Full Text]  
• Becker, W., Weber, Y., Wetzel, K., Eirmbter, K., Tejedor, F. J., Joost, H.-G. (1998). Sequence Characteristics, Subcellular Localization, and Substrate Specificity of DYRK-related Kinases, a Novel Family of Dual Specificity Protein Kinases. J. Biol. Chem. 273: 25893-25902 [Abstract] [Full Text]  
• Katagiri, Y., Hirata, Y., Milbrandt, J., Guroff, G. (1997). Differential Regulation of the Transcriptional Activity of the Orphan Nuclear Receptor NGFI-B by Membrane Depolarization and Nerve Growth Factor. J. Biol. Chem. 272: 31278-31284 [Abstract] [Full Text]  
• Li, Y., Lau, L. F. (1997). Adrenocorticotropic Hormone Regulates the Activities of the Orphan Nuclear Receptor Nur77 through Modulation of Phosphorylation. Endocrinology 138: 4138-4146 [Abstract] [Full Text]