This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Luo, Y Articles by Voellmy, R Search for Related Content PubMed PubMed Citation Articles by Luo, Y Articles by Voellmy, R

Ecdysterone receptor is a sequence-specific transcription factor involved in the developmental regulation of heat shock genes.

Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101.

ABSTRACT

Purification of ecdysterone receptor from Drosophila melanogaster to apparent homogeneity is reported. Purified receptor binds specifically to several sequences in the promoters of the developmentally active hsp27 and hsp23 heat shock genes that were previously implied in ecdysterone regulation of the genes and that share limited homology among themselves and with mammalian steroid receptor binding sites. Some of these elements confer ecdysterone regulation on a basal promoter in transfected cells, acting in a synergistic fashion. Transcription in vitro of promoters containing such elements is stimulated up to 100-fold by added purified ecdysterone receptor, depending on receptor dosage and the number of elements present. Transcriptional enhancement requires sequence-specific binding of receptor to template promoters which facilitates the formation of a preinitiation complex. Ecdysterone stimulates DNA binding of the receptor in vitro.

This article has been cited by other articles:

• Merzendorfer, H., Zimoch, L. (2003). Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J. Exp. Biol. 206: 4393-4412 [Abstract] [Full Text]  
• Simon, A. F., Shih, C., Mack, A., Benzer, S. (2003). Steroid Control of Longevity in Drosophila melanogaster. Science 299: 1407-1410 [Abstract] [Full Text]  
• Reineke, S., Wieczorek, H., Merzendorfer, H. (2002). Expression of Manduca sexta V-ATPase genes mvB, mvG and mvd is regulated by ecdysteroids. J. Exp. Biol. 205: 1059-1067 [Abstract] [Full Text]  
• Wang, S.-F., Ayer, S., Segraves, W. A., Williams, D. R., Raikhel, A. S. (2000). Molecular Determinants of Differential Ligand Sensitivities of Insect Ecdysteroid Receptors. Mol. Cell. Biol. 20: 3870-3879 [Abstract] [Full Text]  
• Wieczorek, H, Grber, G, Harvey, W., Huss, M, Merzendorfer, H, Zeiske, W (2000). Structure and regulation of insect plasma membrane H(+)V-ATPase. J. Exp. Biol. 203: 127-135 [Abstract]  
• Ahmed, A., Martin, D., Manetti, A. G. O., Han, S.-J., Lee, W.-J., Mathiopoulos, K. D., Muller, H.-M., Kafatos, F. C., Raikhel, A., Brey, P. T. (1999). Genomic structure and ecdysone regulation of the prophenoloxidase 1 gene in the malaria vector Anopheles gambiae. Proc. Natl. Acad. Sci. USA 96: 14795-14800 [Abstract] [Full Text]  
• Uusitalo, A., Tenhunen, K., Tenhunen, J., Matikainen, S., Peltonen, L., Jalanko, A. (1997). Expression and Regulation of the Human and Mouse Aspartylglucosaminidase Gene. J. Biol. Chem. 272: 9524-9530 [Abstract] [Full Text]  
• Takeuchi, H., Chen, J.-H., O'Reilly, D. R., Turner, P. C., Rees, H. H. (2001). Regulation of Ecdysteroid Signaling: Cloning and Characterization of Ecdysone Oxidase. A NOVEL STEROID OXIDASE FROM THE COTTON LEAFWORM, SPODOPTERA LITTORALIS. J. Biol. Chem. 276: 26819-26828 [Abstract] [Full Text]  
• Ghbeish, N., Tsai, C.-C., Schubiger, M., Zhou, J. Y., Evans, R. M., McKeown, M. (2001). The dual role of ultraspiracle, the Drosophila retinoid X receptor, in the ecdysone response. Proc. Natl. Acad. Sci. USA 98: 3867-3872 [Abstract] [Full Text]