This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Furter-Graves, E M Articles by Hall, B D Search for Related Content PubMed PubMed Citation Articles by Furter-Graves, E M Articles by Hall, B D

 Previous Article  |  Next Article 

Mol Cell Biol. 1991 August; 11(8): 4121-4127

SHI, a new yeast gene affecting the spacing between TATA and transcription initiation sites.

Department of Genetics, University of Washington, Seattle 98195.

ABSTRACT

In a genetic selection for Saccharomyces cerevisiae genes involved in transcription start site specification, two mutant genes which restore alcohol dehydrogenase activity to a functionally defective S. pombe ADH gene were recovered. Examination of S. pombe ADH initiation sites showed that mutations in the SHI gene shift the location of the transcription initiation window closer to TATA. The shi mutant also affected initiation site selection for two S. cerevisiae genes that were tested. For H2B mRNA, initiation occurred in the shi mutant at a series of initiation sites located 43 to 80 bp 3' of the histone H2B TATA sequence and at the usual initiation sites 102 and 103 bp downstream of the TATA sequence. Weakly used initiation sites ranging from 51 to 80 bp downstream of the TATA sequence were observed for the S. cerevisiae ADH1 gene in shi strains, in addition to the normal ADH1 initiation sites 89 and 99 bp from the TATA sequence. Restoration of function to the defective S. pombe ADH gene occurs only when this gene contains a TATA sequence; a single-base-pair TATA-to-TAGA change is sufficient to prevent this restoration of function. Genetic mapping placed the SHI locus on the left arm of chromosome VII, 22.3 centimorgans from cyh2; it does not correspond to any previously mapped gene.

This article has been cited by other articles:

• Seshadri, V., McArthur, A. G., Sogin, M. L., Adam, R. D. (2003). Giardia lamblia RNA Polymerase II: AMANITIN-RESISTANT TRANSCRIPTION. J. Biol. Chem. 278: 27804-27810 [Abstract] [Full Text]  
• Zhang, D.-Y., Carson, D. J., Ma, J. (2002). The role of TFIIB-RNA polymerase II interaction in start site selection in yeast cells. Nucleic Acids Res 30: 3078-3085 [Abstract] [Full Text]  
• Luo, H., Gilinger, G., Mukherjee, D., Bellofatto, V. (1999). Transcription Initiation at the TATA-less Spliced Leader RNA Gene Promoter Requires at Least Two DNA-binding Proteins and a Tripartite Architecture That Includes an Initiator Element. J. Biol. Chem. 274: 31947-31954 [Abstract] [Full Text]  
• Cho, E.-J., Buratowski, S. (1999). Evidence That Transcription Factor IIB Is Required for a Post-assembly Step in Transcription Initiation. J. Biol. Chem. 274: 25807-25813 [Abstract] [Full Text]  
• Hampsey, M. (1998). Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery. Microbiol. Mol. Biol. Rev. 62: 465-503 [Abstract] [Full Text]  
• Awrey, D. E., Weilbaecher, R. G., Hemming, S. A., Orlicky, S. M., Kane, C. M., Edwards, A. M. (1997). Transcription Elongation through DNA Arrest Sites. A MULTISTEP PROCESS INVOLVING BOTH RNA POLYMERASE II SUBUNIT RPB9 AND TFIIS. J. Biol. Chem. 272: 14747-14754 [Abstract] [Full Text]  
• Hull, M W, McKune, K, Woychik, N A (1995). RNA polymerase II subunit RPB9 is required for accurate start site selection.. Genes Dev. 9: 481-490 [Abstract]  
• Li, Y, Flanagan, P., Tschochner, H, Kornberg, R. (1994). RNA polymerase II initiation factor interactions and transcription start site selection. Science 263: 805-807 [Abstract]