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Mol Cell Biol. 1991 September; 11(9): 4466-4472

The jun and fos protein families are both required for cell cycle progression in fibroblasts.

Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, New Jersey 08543-4000.

ABSTRACT

The expression of different members of the Jun and Fos families of transcription factors is rapidly induced following serum stimulation of quiescent fibroblasts. To determine whether these proteins are required for cell cycle progression, we microinjected affinity-purified antibodies directed against c-Fos, FosB, Fra-1, c-Jun, JunB, and JunD, and antibodies that recognize either the Fos or the Jun family of proteins, into Swiss 3T3 cells and determined their effects in cell cycle progression by monitoring DNA synthesis. We found that microinjection of anti-Fos and anti-Jun family antibodies efficiently blocked the entrance to the S phase of serum-stimulated or asynchronously growing cells. However, the antibodies against single members of the Fos family only partially inhibited DNA synthesis. In contrast, all three Jun antibodies prevented DNA synthesis more effectively than did any of the anti-Fos antibodies.

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• Marinissen, M. J., Servitja, J.-M., Offermanns, S., Simon, M. I., Gutkind, J. S. (2003). Thrombin Protease-activated Receptor-1 Signals through Gq- and G13-initiated MAPK Cascades Regulating c-Jun Expression to Induce Cell Transformation. J. Biol. Chem. 278: 46814-46825 [Abstract] [Full Text]  
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• Meijer, J. H., Schwartz, W. J. (2003). In Search of the Pathways for Light-Induced Pacemaker Resetting in the Suprachiasmatic Nucleus. J Biol Rhythms 18: 235-249 [Abstract]  
• Sun, W., Wei, X., Kesavan, K., Garrington, T. P., Fan, R., Mei, J., Anderson, S. M., Gelfand, E. W., Johnson, G. L. (2003). MEK Kinase 2 and the Adaptor Protein Lad Regulate Extracellular Signal-Regulated Kinase 5 Activation by Epidermal Growth Factor via Src. Mol. Cell. Biol. 23: 2298-2308 [Abstract] [Full Text]  
• Ramos-Nino, M. E., Timblin, C. R., Mossman, B. T. (2002). Mesothelial Cell Transformation Requires Increased AP-1 Binding Activity and ERK-dependent Fra-1 Expression. Cancer Res. 62: 6065-6069 [Abstract] [Full Text]  
• Andrecht, S., Kolbus, A., Hartenstein, B., Angel, P., Schorpp-Kistner, M. (2002). Cell Cycle Promoting Activity of JunB through Cyclin A Activation. J. Biol. Chem. 277: 35961-35968 [Abstract] [Full Text]  
• Chen, S.-Y., Lu, J., Shih, Y.-C., Tsai, C.-H. (2002). Epstein-Barr Virus Latent Membrane Protein 2A Regulates c-Jun Protein through Extracellular Signal-Regulated Kinase. J. Virol. 76: 9556-9561 [Abstract] [Full Text]  
• Medina, D. L., Rivas, M., Cruz, P., Barroso, I., Regadera, J., Santisteban, P. (2002). RhoA Activation Promotes Transformation and Loss of Thyroid Cell Differentiation Interfering with Thyroid Transcription Factor-1 Activity. Mol. Endocrinol. 16: 33-44 [Abstract] [Full Text]  
• Seth, R. K., Haque, M. S. R., Zelenka, P. S. (2001). Regulation of c-fos Induction in Lens Epithelial Cells by 12(S)HETE-Dependent Activation of PKC. IOVS 42: 3239-3246 [Abstract] [Full Text]  
• Kampfer, S., Windegger, M., Hochholdinger, F., Schwaiger, W., Pestell, R. G., Baier, G., Grunicke, H. H., Uberall, F. (2001). Protein Kinase C Isoforms Involved in the Transcriptional Activation of Cyclin D1 by Transforming Ha-Ras. J. Biol. Chem. 276: 42834-42842 [Abstract] [Full Text]  
• Chung, W. H., Bennett, B. M., Racz, W. J., Brien, J. F., Massey, T. E. (2001). Induction of c-jun and TGF-beta 1 in Fischer 344 rats during amiodarone-induced pulmonary fibrosis. Am. J. Physiol. Lung Cell. Mol. Physiol. 281: L1180-L1188 [Abstract] [Full Text]  
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• Hennigan, R. F., Stambrook, P. J. (2001). Dominant Negative c-jun Inhibits Activation of the Cyclin D1 and Cyclin E Kinase Complexes. Mol. Biol. Cell 12: 2352-2363 [Abstract] [Full Text]  
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• Chiariello, M., Marinissen, M. J., Gutkind, J. S. (2000). Multiple Mitogen-Activated Protein Kinase Signaling Pathways Connect the Cot Oncoprotein to the c-jun Promoter and to Cellular Transformation. Mol. Cell. Biol. 20: 1747-1758 [Abstract] [Full Text]  
• Olman, M. A., Hagood, J. S., Simmons, W. L., Fuller, G. M., Vinson, C., White, K. E. (1999). Fibrin Fragment Induction of Plasminogen Activator Inhibitor Transcription Is Mediated by Activator Protein-1 Through a Highly Conserved Element. Blood 94: 2029-2038 [Abstract] [Full Text]  
• Brar, S. S., Kennedy, T. P., Whorton, A. R., Murphy, T. M., Chitano, P., Hoidal, J. R. (1999). Requirement for Reactive Oxygen Species in Serum-induced and Platelet-derived Growth Factor-induced Growth of Airway Smooth Muscle. J. Biol. Chem. 274: 20017-20026 [Abstract] [Full Text]  
• Marinissen, M. J., Chiariello, M., Pallante, M., Gutkind, J. S. (1999). A Network of Mitogen-Activated Protein Kinases Links G Protein-Coupled Receptors to the c-jun Promoter: a Role for c-Jun NH2-Terminal Kinase, p38s, and Extracellular Signal-Regulated Kinase 5. Mol. Cell. Biol. 19: 4289-4301 [Abstract] [Full Text]  
• Murphy, D. J., Hardy, S., Engel, D. A. (1999). Human SWI-SNF Component BRG1 Represses Transcription of the c-fos Gene. Mol. Cell. Biol. 19: 2724-2733 [Abstract] [Full Text]  
• Schreiber, M., Kolbus, A., Piu, F., Szabowski, A., Möhle-Steinlein, U., Tian, J., Karin, M., Angel, P., Wagner, E. F. (1999). Control of cell cycle progression by c-Jun is p53 dependent. Genes Dev. 13: 607-619 [Abstract] [Full Text]  
• Okada, S., Fukuda, T., Inada, K., Tokuhisa, T. (1999). Prolonged Expression of c-fos Suppresses Cell Cycle Entry of Dormant Hematopoietic Stem Cells. Blood 93: 816-825 [Abstract] [Full Text]  
• Kustikova, O., Kramerov, D., Grigorian, M., Berezin, V., Bock, E., Lukanidin, E., Tulchinsky, E. (1998). Fra-1 Induces Morphological Transformation and Increases In Vitro Invasiveness and Motility of Epithelioid Adenocarcinoma Cells. Mol. Cell. Biol. 18: 7095-7105 [Abstract] [Full Text]  
• Brown, J. R., Nigh, E., Lee, R. J., Ye, H., Thompson, M. A., Saudou, F., Pestell, R. G., Greenberg, M. E. (1998). Fos Family Members Induce Cell Cycle Entry by Activating Cyclin D1. Mol. Cell. Biol. 18: 5609-5619 [Abstract] [Full Text]  
• Lu, C., Giordano, F. J., Bao, X., Morris, K. C., Rothman, A. (1998). Antisense fosB RNA Inhibits Thrombin-Induced Hypertrophy in Cultured Pulmonary Arterial Smooth Muscle Cells. Circulation 98: 596-603 [Abstract] [Full Text]  
• Herdegen, T., Claret, F.-X., Kallunki, T., Martin-Villalba, A., Winter, C., Hunter, T., Karin, M. (1998). Lasting N-Terminal Phosphorylation of c-Jun and Activation of c-Jun N-Terminal Kinases after Neuronal Injury. J. Neurosci. 18: 5124-5135 [Abstract] [Full Text]  
• Yubero, P., Barberá, M., Alvarez, R., Viñas, O., Mampel, T., Iglesias, R., Villarroya, F., Giralt, M. (1998). Dominant Negative Regulation by c-Jun of Transcription of the Uncoupling Protein-1 Gene through a Proximal cAMP-Regulatory Element: A Mechanism for Repressing Basal and Norepinephrine-Induced Expression of the Gene before Brown Adipocyte Differentiation. Mol. Endocrinol. 12: 1023-1037 [Abstract] [Full Text]  
• Clarke, N., Arenzana, N., Hai, T., Minden, A., Prywes, R. (1998). Epidermal Growth Factor Induction of the c-jun Promoter by a Rac Pathway. Mol. Cell. Biol. 18: 1065-1073 [Abstract] [Full Text]  
• Lotfi, C. F. P., Todorovic, Z., Armelin, H. A., Schimmer, B. P. (1997). Unmasking a Growth-promoting Effect of the Adrenocorticotropic Hormone in Y1 Mouse Adrenocortical Tumor Cells. J. Biol. Chem. 272: 29886-29891 [Abstract] [Full Text]  
• Coso, O. A., Montaner, S., Fromm, C., Lacal, J. C., Prywes, R., Teramoto, H., Gutkind, J. S. (1997). Signaling from G Protein-coupled Receptors to the c-jun Promoter Involves the MEF2 Transcription Factor. EVIDENCE FOR A NOVEL c-Jun AMINO-TERMINAL KINASE-INDEPENDENT PATHWAY. J. Biol. Chem. 272: 20691-20697 [Abstract] [Full Text]  
• Mitsui, H., Takuwa, N., Kurokawa, K., Exton, J. H., Takuwa, Y. (1997). Dependence of Activated Galpha 12-induced G1 to S Phase Cell Cycle Progression on Both Ras/Mitogen-activated Protein Kinase and Ras/Rac1/Jun N-terminal Kinase Cascades in NIH3T3 Fibroblasts. J. Biol. Chem. 272: 4904-4910 [Abstract] [Full Text]  
• Hales, B. F. (1997). Oxidative Stress Regulates the Expression and Activity of Transcription Factor Activator Protein-1 in Rat Conceptus. J. Pharmacol. Exp. Ther. 280: 1085-1093 [Abstract] [Full Text]  
• Hsu, D. K.W., Guo, Y., Alberts, G. F., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Peifley, K. A., Winkles, J. A. (1996). Identification of a Murine TEF-1-related Gene Expressed after Mitogenic Stimulation of Quiescent Fibroblasts and during Myogenic Differentiation. J. Biol. Chem. 271: 13786-13795 [Abstract] [Full Text]  
• Chen, Y.-Q., Gilliam, D. M., Rydzewski, B., Naftilan, A. J. (1996). Multiple Enhancer Elements Mediate Induction of c-fos in Vascular Smooth Muscle Cells. Hypertension 27: 1224-1233 [Abstract] [Full Text]  
• Albanese, C., Johnson, J., Watanabe, G., Eklund, N., Vu, D., Arnold, A., Pestell, R. G. (1995). Transforming p21[IMAGE] Mutants and c-Ets-2 Activate the Cyclin D1 Promoter through Distinguishable Regions. J. Biol. Chem. 270: 23589-23597 [Abstract] [Full Text]  
• Puri, P. L., Avantaggiati, M. L., Burgio, V. L., Chirillo, P., Collepardo, D., Natoli, G., Balsano, C., Levrero, M. (1995). Reactive Oxygen Intermediates Mediate Angiotensin II-induced c-Junbulletc-Fos Heterodimer DNA Binding Activity and Proliferative Hypertrophic Responses in Myogenic Cells. J. Biol. Chem. 270: 22129-22134 [Abstract] [Full Text]  
• Rezzonico, R., Ponzio, G., Loubat, A.ès, Lallemand, D., Proudfoot, A., Rossi, B. (1995). Two Distinct Signalling Pathways Are Involved in the Control of the Biphasic junB Transcription Induced by Interleukin-6 in the B Cell Hybridoma 7TD1. J. Biol. Chem. 270: 1261-1268 [Abstract] [Full Text]  
• Barnett, S., Rosario, M, Doyle, A, Kilbey, A, Lovatt, A, Gillespie, D. (1995). Differential regulation of AP-1 and novel TRE-specific DNA-binding complexes during differentiation of oligodendrocyte-type-2-astrocyte (O-2A) progenitor cells. Development 121: 3969-3977 [Abstract]  
• Carrasco, D, Weih, F, Bravo, R (1994). Developmental expression of the mouse c-rel proto-oncogene in hematopoietic organs. Development 120: 2991-3004 [Abstract]  
• Wick, M, Burger, C, Brusselbach, S, Lucibello, F., Muller, R (1994). Identification of serum-inducible genes: different patterns of gene regulation during G0-->S and G1-->S progression. J. Cell Sci. 107: 227-239 [Abstract]  
• Siegele, D A, Kolter, R (1993). Isolation and characterization of an Escherichia coli mutant defective in resuming growth after starvation.. Genes Dev. 7: 2629-2640 [Abstract]  
• Johnson, R S, van Lingen, B, Papaioannou, V E, Spiegelman, B M (1993). A null mutation at the c-jun locus causes embryonic lethality and retarded cell growth in culture.. Genes Dev. 7: 1309-1317 [Abstract]  
• Carrasco, D, Ryseck, R., Bravo, R (1993). Expression of relB transcripts during lymphoid organ development: specific expression in dendritic antigen-presenting cells. Development 118: 1221-1231 [Abstract]  
• Adolph, S, Brusselbach, S, Muller, R (1993). Inhibition of transcription blocks cell cycle progression of NIH3T3 fibroblasts specifically in G1. J. Cell Sci. 105: 113-122 [Abstract]  
• Papavassiliou, A., Treier, M, Chavrier, C, Bohmann, D (1992). Targeted degradation of c-Fos, but not v-Fos, by a phosphorylation-dependent signal on c-Jun. Science 258: 1941-1944 [Abstract]  
• Kato, Y., Zhao, M., Morikawa, A., Sugiyama, T., Chakravortty, D., Koide, N., Yoshida, T., Tapping, R. I., Yang, Y., Yokochi, T., Lee, J.-D. (2000). Big Mitogen-activated Kinase Regulates Multiple Members of the MEF2 Protein Family. J. Biol. Chem. 275: 18534-18540 [Abstract] [Full Text]  
• Sun, W., Kesavan, K., Schaefer, B. C., Garrington, T. P., Ware, M., Johnson, N. L., Gelfand, E. W., Johnson, G. L. (2001). MEKK2 Associates with the Adapter Protein Lad/RIBP and Regulates the MEK5-BMK1/ERK5 Pathway. J. Biol. Chem. 276: 5093-5100 [Abstract] [Full Text]  
• Singh, P., Chan, S. W., Hong, W. (2001). Retinoblastoma Protein Is Functionally Distinct from Its Homologues in Affecting Glucocorticoid Receptor-mediated Transcription and Apoptosis. J. Biol. Chem. 276: 13762-13770 [Abstract] [Full Text]  
• Kumar, N. V., Bernstein, L. R. (2001). Ten ERK-related Proteins in Three Distinct Classes Associate with AP-1 Proteins and/or AP-1 DNA. J. Biol. Chem. 276: 32362-32372 [Abstract] [Full Text]