This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matunis, M J
Right arrow Articles by Dreyfuss, G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matunis, M J
Right arrow Articles by Dreyfuss, G

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 January; 12(1): 164-171

Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein.

M J Matunis, W M Michael and G Dreyfuss

Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104-6148.

ABSTRACT

At least 20 major proteins make up the ribonucleoprotein (RNP) complexes of heterogeneous nuclear RNA (hnRNA) in mammalian cells. Many of these proteins have distinct RNA-binding specificities. The abundant, acidic heterogeneous nuclear RNP (hnRNP) K and J proteins (66 and 64 kDa, respectively, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) are unique among the hnRNP proteins in their binding preference: they bind tenaciously to poly(C), and they are the major oligo(C)- and poly(C)-binding proteins in human HeLa cells. We purified K and J from HeLa cells by affinity chromatography and produced monoclonal antibodies to them. K and J are immunologically related and conserved among various vertebrates. Immunofluorescence microscopy with antibodies shows that K and J are located in the nucleoplasm. cDNA clones for K were isolated, and their sequences were determined. The predicted amino acid sequence of K does not contain an RNP consensus sequence found in many characterized hnRNP proteins and shows no extensive homology to sequences of any known proteins. The K protein contains two internal repeats not found in other known proteins, as well as GlyArgGlyGly and GlyArgGlyGlyPhe sequences, which occur frequently in many RNA-binding proteins. Overall, K represents a novel type of hnRNA-binding protein. It is likely that K and J play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences.

Mol Cell Biol. 1992 January; 12(1): 164-171




This article has been cited by other articles:

  • Mukhopadhyay, N. K., Kim, J., Cinar, B., Ramachandran, A., Hager, M. H., Di Vizio, D., Adam, R. M., Rubin, M. A., Raychaudhuri, P., De Benedetti, A., Freeman, M. R. (2009). Heterogeneous Nuclear Ribonucleoprotein K Is a Novel Regulator of Androgen Receptor Translation. Cancer Res. 69: 2210-2218 [Abstract] [Full Text]  
  • Wang, L. G., Johnson, E. M., Kinoshita, Y., Babb, J. S., Buckley, M. T., Liebes, L. F., Melamed, J., Liu, X.-M., Kurek, R., Ossowski, L., Ferrari, A. C. (2008). Androgen Receptor Overexpression in Prostate Cancer Linked to Pur{alpha} Loss from a Novel Repressor Complex. Cancer Res. 68: 2678-2688 [Abstract] [Full Text]  
  • Feliers, D., Lee, M.-J., Ghosh-Choudhury, G., Bomsztyk, K., Kasinath, B. S. (2007). Heterogeneous nuclear ribonucleoprotein K contributes to angiotensin II stimulation of vascular endothelial growth factor mRNA translation. Am. J. Physiol. Renal Physiol. 293: F607-F615 [Abstract] [Full Text]  
  • Yaklichkin, S., Steiner, A. B., Lu, Q., Kessler, D. S. (2007). FoxD3 and Grg4 Physically Interact to Repress Transcription and Induce Mesoderm in Xenopus. J. Biol. Chem. 282: 2548-2557 [Abstract] [Full Text]  
  • Emerald, B. S., Chen, Y., Zhu, T., Zhu, Z., Lee, K.-O., Gluckman, P. D., Lobie, P. E. (2007). {alpha}CP1 Mediates Stabilization of hTERT mRNA by Autocrine Human Growth Hormone. J. Biol. Chem. 282: 680-690 [Abstract] [Full Text]  
  • Tomoiu, A., Gravel, A., Tanguay, R. M., Flamand, L. (2006). Functional Interaction between Human Herpesvirus 6 Immediate-Early 2 Protein and Ubiquitin-Conjugating Enzyme 9 in the Absence of Sumoylation.. J. Virol. 80: 10218-10228 [Abstract] [Full Text]  
  • Wei, C.-C., Zhang, S.-L., Chen, Y.-W., Guo, D.-F., Ingelfinger, J. R., Bomsztyk, K., Chan, J. S. D. (2006). Heterogeneous Nuclear Ribonucleoprotein K Modulates Angiotensinogen Gene Expression in Kidney Cells. J. Biol. Chem. 281: 25344-25355 [Abstract] [Full Text]  
  • Kong, J., Sumaroka, M., Eastmond, D. L., Liebhaber, S. A. (2006). Shared Stabilization Functions of Pyrimidine-Rich Determinants in the Erythroid 15-lipoxygenase and {alpha}-globin mRNAs. Mol. Cell. Biol. 26: 5603-5614 [Abstract] [Full Text]  
  • Yoo, Y., Wu, X., Egile, C., Li, R., Guan, J.-L. (2006). Interaction of N-WASP with hnRNPK and Its Role in Filopodia Formation and Cell Spreading. J. Biol. Chem. 281: 15352-15360 [Abstract] [Full Text]  
  • Harris, D., Zhang, Z., Chaubey, B., Pandey, V. N. (2006). Identification of Cellular Factors Associated with the 3'-Nontranslated Region of the Hepatitis C Virus Genome. Mol. Cell. Proteomics 5: 1006-1018 [Abstract] [Full Text]  
  • Ostareck-Lederer, A., Ostareck, D. H., Rucknagel, K. P., Schierhorn, A., Moritz, B., Huttelmaier, S., Flach, N., Handoko, L., Wahle, E. (2006). Asymmetric Arginine Dimethylation of Heterogeneous Nuclear Ribonucleoprotein K by Protein-arginine Methyltransferase 1 Inhibits Its Interaction with c-Src. J. Biol. Chem. 281: 11115-11125 [Abstract] [Full Text]  
  • Zhang, T., Delestienne, N., Huez, G., Kruys, V., Gueydan, C. (2005). Identification of the sequence determinants mediating the nucleo-cytoplasmic shuttling of TIAR and TIA-1 RNA-binding proteins. J. Cell Sci. 118: 5453-5463 [Abstract] [Full Text]  
  • Kim, S.-S., Pandey, K. K., Choi, H. S., Kim, S.-Y., Law, P.-Y., Wei, L.-N., Loh, H. H. (2005). Poly(C) Binding Protein Family Is a Transcription Factor in {micro}-Opioid Receptor Gene Expression. Mol. Pharmacol. 68: 729-736 [Abstract] [Full Text]  
  • Musunuru, K., Darnell, R. B. (2004). Determination and augmentation of RNA sequence specificity of the Nova K-homology domains. Nucleic Acids Res 32: 4852-4861 [Abstract] [Full Text]  
  • Boopathi, E., Lenka, N., Prabu, S. K., Fang, J.-K., Wilkinson, F., Atchison, M., Giallongo, A., Avadhani, N. G. (2004). Regulation of Murine Cytochrome c Oxidase Vb Gene Expression during Myogenesis: YY-1 AND HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN D-LIKE PROTEIN (JKTBP1) RECIPROCALLY REGULATE TRANSCRIPTION ACTIVITY BY PHYSICAL INTERACTION WITH THE BERF-1/ZBP-89 FACTOR. J. Biol. Chem. 279: 35242-35254 [Abstract] [Full Text]  
  • Chkheidze, A. N., Liebhaber, S. A. (2003). A Novel Set of Nuclear Localization Signals Determine Distributions of the {alpha}CP RNA-Binding Proteins. Mol. Cell. Biol. 23: 8405-8415 [Abstract] [Full Text]  
  • Thakur, S., Nakamura, T., Calin, G., Russo, A., Tamburrino, J. F., Shimizu, M., Baldassarre, G., Battista, S., Fusco, A., Wassell, R. P., Dubois, G., Alder, H., Croce, C. M. (2003). Regulation of BRCA1 Transcription by Specific Single-Stranded DNA Binding Factors. Mol. Cell. Biol. 23: 3774-3787 [Abstract] [Full Text]  
  • Ji, X., Kong, J., Liebhaber, S. A. (2003). In Vivo Association of the Stability Control Protein {alpha}CP with Actively Translating mRNAs. Mol. Cell. Biol. 23: 899-907 [Abstract] [Full Text]  
  • Da Silva, N., Bharti, A., Shelley, C. S. (2002). hnRNP-K and Puralpha act together to repress the transcriptional activity of the CD43 gene promoter. Blood 100: 3536-3544 [Abstract] [Full Text]  
  • Chen, H.-C., Lin, W.-C., Tsay, Y.-G., Lee, S.-C., Chang, C.-J. (2002). An RNA Helicase, DDX1, Interacting with Poly(A) RNA and Heterogeneous Nuclear Ribonucleoprotein K. J. Biol. Chem. 277: 40403-40409 [Abstract] [Full Text]  
  • Gustin, K. E., Sarnow, P. (2002). Inhibition of Nuclear Import and Alteration of Nuclear Pore Complex Composition by Rhinovirus. J. Virol. 76: 8787-8796 [Abstract] [Full Text]  
  • Expert-Bezancon, A., Le Caer, J. P., Marie, J. (2002). Heterogeneous Nuclear Ribonucleoprotein (hnRNP) K Is a Component of an Intronic Splicing Enhancer Complex That Activates the Splicing of the Alternative Exon 6A from Chicken beta -Tropomyosin Pre-mRNA. J. Biol. Chem. 277: 16614-16623 [Abstract] [Full Text]  
  • Denisenko, O., Bomsztyk, K. (2002). Yeast hnRNP K-Like Genes Are Involved in Regulation of the Telomeric Position Effect and Telomere Length. Mol. Cell. Biol. 22: 286-297 [Abstract] [Full Text]  
  • Mili, S., Shu, H. J., Zhao, Y., Pinol-Roma, S. (2001). Distinct RNP Complexes of Shuttling hnRNP Proteins with Pre-mRNA and mRNA: Candidate Intermediates in Formation and Export of mRNA. Mol. Cell. Biol. 21: 7307-7319 [Abstract] [Full Text]  
  • Guillonneau, F., Guieysse, A. L., Le Caer, J. P., Rossier, J., Praseuth, D. (2001). Selection and identification of proteins bound to DNA triple-helical structures by combination of 2D-electrophoresis and MALDI-TOF mass spectrometry. Nucleic Acids Res 29: 2427-2436 [Abstract] [Full Text]  
  • Holcik, M., Korneluk, R. G. (2000). Functional Characterization of the X-Linked Inhibitor of Apoptosis (XIAP) Internal Ribosome Entry Site Element: Role of La Autoantigen in XIAP Translation. Mol. Cell. Biol. 20: 4648-4657 [Abstract] [Full Text]  
  • Shnyreva, M., Schullery, D. S., Suzuki, H., Higaki, Y., Bomsztyk, K. (2000). Interaction of Two Multifunctional Proteins. HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN K AND Y-BOX-BINDING PROTEIN. J. Biol. Chem. 275: 15498-15503 [Abstract] [Full Text]  
  • Lacroix, L., Lienard, H., Labourier, E., Djavaheri-Mergny, M., Lacoste, J., Leffers, H., Tazi, J., Helene, C., Mergny, J.-L. (2000). Identification of two human nuclear proteins that recognise the cytosine-rich strand of human telomeres in vitro. Nucleic Acids Res 28: 1564-1575 [Abstract] [Full Text]  
  • Ostrowski, J., Schullery, D. S., Denisenko, O. N., Higaki, Y., Watts, J., Aebersold, R., Stempka, L., Gschwendt, M., Bomsztyk, K. (2000). Role of Tyrosine Phosphorylation in the Regulation of the Interaction of Heterogenous Nuclear Ribonucleoprotein K Protein with Its Protein and RNA Partners. J. Biol. Chem. 275: 3619-3628 [Abstract] [Full Text]  
  • Melcak, I., Koberna, K., Malínský, J. (2000). Nuclear pre-mRNA Compartmentalization: Trafficking of Released Transcripts to Splicing Factor Reservoirs. Mol. Biol. Cell 11: 497-510 [Abstract] [Full Text]  
  • Pazman, C, Mayes, C., Fanto, M, Haynes, S., Mlodzik, M (2000). Rasputin, the Drosophila homologue of the RasGAP SH3 binding protein, functions in ras- and Rho-mediated signaling. Development 127: 1715-1725 [Abstract]  
  • Charroux, B., Angelats, C., Fasano, L., Kerridge, S., Vola, C. (1999). The Levels of the bancal Product, a Drosophila Homologue of Vertebrate hnRNP K Protein, Affect Cell Proliferation and Apoptosis in Imaginal Disc Cells. Mol. Cell. Biol. 19: 7846-7856 [Abstract] [Full Text]  
  • Ai, L.-S., Chau, L.-Y. (1999). Post-transcriptional Regulation of H-ferritin mRNA. IDENTIFICATION OF A PYRIMIDINE-RICH SEQUENCE IN THE 3'-UNTRANSLATED REGION ASSOCIATED WITH MESSAGE STABILITY IN HUMAN MONOCYTIC THP-1 CELLS. J. Biol. Chem. 274: 30209-30214 [Abstract] [Full Text]  
  • Wadd, S., Bryant, H., Filhol, O., Scott, J. E., Hsieh, T.-Y., Everett, R. D., Clements, J. B. (1999). The Multifunctional Herpes Simplex Virus IE63 Protein Interacts with Heterogeneous Ribonucleoprotein K and with Casein Kinase 2. J. Biol. Chem. 274: 28991-28998 [Abstract] [Full Text]  
  • Chkheidze, A. N., Lyakhov, D. L., Makeyev, A. V., Morales, J., Kong, J., Liebhaber, S. A. (1999). Assembly of the alpha -Globin mRNA Stability Complex Reflects Binary Interaction between the Pyrimidine-Rich 3' Untranslated Region Determinant and Poly(C) Binding Protein alpha CP. Mol. Cell. Biol. 19: 4572-4581 [Abstract] [Full Text]  
  • Schullery, D. S., Ostrowski, J., Denisenko, O. N., Stempka, L., Shnyreva, M., Suzuki, H., Gschwendt, M., Bomsztyk, K. (1999). Regulated Interaction of Protein Kinase Cdelta with the Heterogeneous Nuclear Ribonucleoprotein K Protein. J. Biol. Chem. 274: 15101-15109 [Abstract] [Full Text]  
  • Kim, C.-Y., Takahashi, K., Nguyen, T. B., Roberts, J. K. M., Webster, C. (1999). Identification of a Nucleic Acid Binding Domain in Eukaryotic Initiation Factor eIFiso4G from Wheat. J. Biol. Chem. 274: 10603-10608 [Abstract] [Full Text]  
  • Ullman, K. S., Shah, S., Powers, M. A., Forbes, D. J. (1999). The Nucleoporin Nup153 Plays a Critical Role in Multiple Types of Nuclear Export. Mol. Biol. Cell 10: 649-664 [Abstract] [Full Text]  
  • Collier, B., Goobar-Larsson, L., Sokolowski, M., Schwartz, S. (1998). Translational Inhibition in Vitro of Human Papillomavirus Type 16 L2 mRNA Mediated through Interaction with Heterogenous Ribonucleoprotein K and Poly(rC)-binding Proteins 1 and 2. J. Biol. Chem. 273: 22648-22656 [Abstract] [Full Text]  
  • Saffman, E. E., Styhler, S., Rother, K., Li, W., Richard, S., Lasko, P. (1998). Premature Translation of oskar in Oocytes Lacking the RNA-Binding Protein Bicaudal-C. Mol. Cell. Biol. 18: 4855-4862 [Abstract] [Full Text]  
  • Du, Q., Melnikova, I. N., Gardner, P. D. (1998). Differential Effects of Heterogeneous Nuclear Ribonucleoprotein K on Sp1- and Sp3-mediated Transcriptional Activation of a Neuronal Nicotinic Acetylcholine Receptor Promoter. J. Biol. Chem. 273: 19877-19883 [Abstract] [Full Text]  
  • Hsieh, T.-Y., Matsumoto, M., Chou, H.-C., Schneider, R., Hwang, S. B., Lee, A. S., Lai, M. M. C. (1998). Hepatitis C Virus Core Protein Interacts with Heterogeneous Nuclear Ribonucleoprotein K. J. Biol. Chem. 273: 17651-17659 [Abstract] [Full Text]  
  • Kanamori, H., Dodson, R. E., Shapiro, D. J. (1998). In Vitro Genetic Analysis of the RNA Binding Site of Vigilin, a Multi-KH-Domain Protein. Mol. Cell. Biol. 18: 3991-4003 [Abstract] [Full Text]  
  • Delling, U., Raymond, M., Schurr, E. (1998). Identification of Saccharomyces cerevisiae Genes Conferring Resistance to Quinoline Ring-Containing Antimalarial Drugs. Antimicrob. Agents Chemother. 42: 1034-1041 [Abstract] [Full Text]  
  • Miau, L.-H., Chang, C.-J., Shen, B.-J., Tsai, W.-H., Lee, S.-C. (1998). Identification of Heterogeneous Nuclear Ribonucleoprotein K (hnRNP K) as a Repressor of C/EBPbeta -mediated Gene Activation. J. Biol. Chem. 273: 10784-10791 [Abstract] [Full Text]  
  • Nishiyama, H., Itoh, K., Kaneko, Y., Kishishita, M., Yoshida, O., Fujita, J. (1997). A Glycine-rich RNA-binding Protein Mediating Cold-inducible Suppression of Mammalian Cell Growth. JCB 137: 899-908 [Abstract] [Full Text]  
  • Buchenau, P., Saumweber, H., Arndt-Jovin, D. J. (1997). The Dynamic Nuclear Redistribution of an hnRNP K-homologous Protein during Drosophila Embryo Development and Heat Shock. Flexibility of Transcription Sites In Vivo. JCB 137: 291-303 [Abstract] [Full Text]  
  • Denisenko, O. N., O'Neill, B., Ostrowski, J., Van Seuningen, I., Bomsztyk, K. (1996). Zik1, a Transcriptional Repressor That Interacts with the Heterogeneous Nuclear Ribonucleoprotein Particle K Protein. J. Biol. Chem. 271: 27701-27706 [Abstract] [Full Text]  
  • Sun, X.-L., Sun, X. L. (1996). Evidence That a Specific Interaction Between an 18-Base cis-Element in the 5'-Untranslated Region of Human Folate Receptor-alpha mRNA and a 46-kDa Cytosolic trans-Factor is Critical for Translation. J. Biol. Chem. 271: 25539-25547 [Abstract] [Full Text]  
  • Bunnell, S. C., Henry, P. A., Kolluri, R., Kirchhausen, T., Rickles, R. J., Berg, L. J. (1996). Identification of Itk/Tsk Src Homology 3Domain Ligands. J. Biol. Chem. 271: 25646-25656 [Abstract] [Full Text]  
  • Michael, W. M., Dreyfuss, G. (1996). Distinct Domains in Ribosomal Protein L5 Mediate 5 S rRNA Binding and Nucleolar Localization. J. Biol. Chem. 271: 11571-11574 [Abstract] [Full Text]  
  • Lee, M.-H., Mori, S., Raychaudhuri, P. (1996). trans-Activation by the hnRNP K Protein Involves an Increase in RNA Synthesis from the Reporter Genes. J. Biol. Chem. 271: 3420-3427 [Abstract] [Full Text]  
  • Czyzyk-Krzeska, M. F., Beresh, J. E. (1996). Characterization of the Hypoxia-inducible Protein Binding Site within the Pyrimidine-rich Tract in the 3`-Untranslated Region of the Tyrosine Hydroxylase mRNA. J. Biol. Chem. 271: 3293-3299 [Abstract] [Full Text]  
  • Van Seuningen, I., Ostrowski, J., Bustelo, X. R., Sleath, P. R., Bomsztyk, K. (1995). The K Protein Domain That Recruits the Interleukin 1-responsive K Protein Kinase Lies Adjacent to a Cluster of c-Src and Vav SH3-binding Sites. J. Biol. Chem. 270: 26976-26985 [Abstract] [Full Text]  
  • Liu, X, Mertz, J E (1995). HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.. Genes Dev. 9: 1766-1780 [Abstract]  
  • Michelotti, E. F., Tomonaga, T., Krutzsch, H., Levens, D. (1995). Cellular Nucleic Acid Binding Protein Regulates the CT Element of the Human c- myc Protooncogene. J. Biol. Chem. 270: 9494-9499 [Abstract] [Full Text]  
  • Tomonaga, T., Levens, D. (1995). Heterogeneous Nuclear Ribonucleoprotein K Is a DNA-binding Transactivator. J. Biol. Chem. 270: 4875-4881 [Abstract] [Full Text]  
  • Siebel, C W, Admon, A, Rio, D C (1995). Soma-specific expression and cloning of PSI, a negative regulator of P element pre-mRNA splicing.. Genes Dev. 9: 269-283 [Abstract]  
  • Solovei, I, Macgregor, H, Gaginskaya, E (1995). Single stranded nucleic acid binding structures on chicken lampbrush chromosomes. J. Cell Sci. 108: 1391-1396 [Abstract]  
  • Burd, C., Dreyfuss, G (1994). Conserved structures and diversity of functions of RNA-binding proteins. Science 265: 615-621 [Abstract]  
  • Duncan, R, Bazar, L, Michelotti, G, Tomonaga, T, Krutzsch, H, Avigan, M, Levens, D (1994). A sequence-specific, single-strand binding protein activates the far upstream element of c-myc and defines a new DNA-binding motif.. Genes Dev. 8: 465-480 [Abstract]  
  • Ashley, C. Jr, Wilkinson, K., Reines, D, Warren, S. (1993). FMR1 protein: conserved RNP family domains and selective RNA binding. Science 262: 563-566 [Abstract]  
  • Garcia-Gras, E. A., Chi, P., Thompson, E. A. (2000). Glucocorticoid-mediated Destabilization of Cyclin D3 mRNA Involves RNA-Protein Interactions in the 3'-Untranslated Region of the mRNA. J. Biol. Chem. 275: 22001-22008 [Abstract] [Full Text]  
  • Ko, J. L., Loh, H. H. (2001). Single-stranded DNA-binding Complex Involved in Transcriptional Regulation of Mouse {micro}-Opioid Receptor Gene. J. Biol. Chem. 276: 788-795 [Abstract] [Full Text]  
  • Mandal, M., Vadlamudi, R., Nguyen, D., Wang, R.-A., Costa, L., Bagheri-Yarmand, R., Mendelsohn, J., Kumar, R. (2001). Growth Factors Regulate Heterogeneous Nuclear Ribonucleoprotein K Expression and Function. J. Biol. Chem. 276: 9699-9704 [Abstract] [Full Text]  
  • Fabini, G., Raijmakers, R., Hayer, S., Fouraux, M. A., Pruijn, G. J. M., Steiner, G. (2001). The Heterogeneous Nuclear Ribonucleoproteins I and K Interact with a Subset of the Ro Ribonucleoprotein-associated Y RNAs in Vitro and in Vivo. J. Biol. Chem. 276: 20711-20718 [Abstract] [Full Text]  
  • Xiao, X., Tang, Y.-S., Mackins, J. Y., Sun, X.-L., Jayaram, H. N., Hansen, D. K., Antony, A. C. (2001). Isolation and Characterization of a Folate Receptor mRNA-binding trans-Factor from Human Placenta. EVIDENCE FAVORING IDENTITY WITH HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN E1. J. Biol. Chem. 276: 41510-41517 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»