This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mietz, J A Articles by Kuff, E L Search for Related Content PubMed PubMed Citation Articles by Mietz, J A Articles by Kuff, E L

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 January; 12(1): 220-228

Selective activation of a discrete family of endogenous proviral elements in normal BALB/c lymphocytes.

Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892.

ABSTRACT

Intracisternal A-particle (IAP) proviral elements are abundant and widely dispersed in the mouse genome. IAP-related transcripts have been detected in normal mouse tissues where expression is under genetic control. In this study, we sought to determine whether IAP expression in BALB/c thymus and lipopolysaccharide-stimulated B cells was due to selective or indiscriminate activation of IAP elements. cDNA libraries were prepared from each source. A total of 86 IAP cDNA clones were isolated from both libraries, and 37 of these were sequenced over a common 0.7- to 1.0-kb region of the IAP genome that included the 3' long terminal repeat (LTR). Three highly related families of elements were found to be expressed in the two cell types examined. All of the related elements had a distinctive U3 regulatory region. Thirteen individual IAP proviral elements were distinguished on the basis of sequence differences within the R region of the LTR. Hybridization of genomic DNA with element-specific oligonucleotide probes confirmed the presence of a restricted number of proviral copies in the lymphocyte-specific family of elements. Most of these copies were found to be methylated in the lymphocyte DNA, but at least seven were hypomethylated in their 5' LTRs. This study shows that activation of IAP elements in normal normal mouse lymphocytes is highly selective. Activation is probably a function of both sequence specificity and methylation status of the proviral LTR.

This article has been cited by other articles:

• Maksakova, I. A., Mager, D. L. (2005). Transcriptional Regulation of Early Transposon Elements, an Active Family of Mouse Long Terminal Repeat Retrotransposons. J. Virol. 79: 13865-13874 [Abstract] [Full Text]  
• Barbot, W., Dupressoir, A., Lazar, V., Heidmann, T. (2002). Epigenetic regulation of an IAP retrotransposon in the aging mouse: progressive demethylation and de-silencing of the element by its repetitive induction. Nucleic Acids Res 30: 2365-2373 [Abstract] [Full Text]  
• Gwynn, B., Lueders, K., Sands, M. S., Birkenmeier, E. H. (1998). Intracisternal A-Particle Element Transposition into the Murine beta -Glucuronidase Gene Correlates with Loss of Enzyme Activity: a New Model for beta -Glucuronidase Deficiency in the C3H Mouse. Mol. Cell. Biol. 18: 6474-6481 [Abstract] [Full Text]  
• Muller, J. R., Giese, T., Henry, D. L., Mushinski, J. F., Marcu, K. B. (1998). Generation of Switch Hybrid DNA Between Ig Heavy Chain-{micro} and Downstream Switch Regions in B Lymphocytes. J. Immunol. 161: 1354-1362 [Abstract] [Full Text]  
• Puech, A., Dupressoir, A., Loireau, M.-P., Mattei, M.-G., Heidmann, T. (1997). Characterization of Two Age-induced Intracisternal A-particle-related Transcripts in the Mouse Liver. TRANSCRIPTIONAL READ-THROUGH INTO AN OPEN READING FRAME WITH SIMILARITIES TO THE YEAST CCR4 TRANSCRIPTION FACTOR. J. Biol. Chem. 272: 5995-6003 [Abstract] [Full Text]  
• Kawaji, H., Schonbach, C., Matsuo, Y., Kawai, J., Okazaki, Y., Hayashizaki, Y., Matsuda, H. (2002). Exploration of Novel Motifs Derived from Mouse cDNA Sequences. Genome Res 12: 367-378 [Abstract] [Full Text]