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Mol Cell Biol. 1992 October; 12(10): 4422-4432

Ectopic recombination within homologous immunoglobulin mu gene constant regions in a mouse hybridoma cell line.

Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.

ABSTRACT

We have transferred a pSV2neo vector containing the wild-type constant region of the immunoglobulin mu gene (C mu) into the mutant hybridoma igm482, which bears a 2-bp deletion in the third constant-region exon of its haploid chromosomal mu gene (C mu 3). Independent igm482 transformants contain the wild-type immunoglobulin C mu region stably integrated in ectopic chromosomal positions. We report here that the wild-type immunoglobulin C mu region can function as the donor sequence in a gene conversion event which corrects the 2-bp deletion in the mutant igm482 chromosomal C mu 3 exon. The homologous recombination event restores normal immunoglobulin M production in the mutant cell.

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