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Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae.

Department of Cellular, Viral, and Molecular Biology, University of Utah Medical Center, Salt Lake City 84132.

ABSTRACT

We have cloned and sequenced the SIN4 gene and determined that SIN4 is identical to TSF3, identified as a negative regulator of GAL1 gene transcription (S. Chen, R.W. West, Jr., S.L. Johnson, H. Gans, and J. Ma, submitted for publication). Yeast strains bearing a sin4 delta null mutation have been constructed and are temperature sensitive for growth and display defects in both negative and positive regulation of transcription. Transcription of the CTS1 gene is reduced in sin4 delta mutants, suggesting that Sin4 functions as a positive transcriptional regulator. Additionally, a Sin4-LexA fusion protein activates transcription from test promoters containing LexA binding sites. The sin4 delta mutant also shows phenotypes common to histone and spt mutants, including suppression of delta insertion mutations in the HIS4 and LYS2 promoters, expression of promoters lacking upstream activation sequence elements, and decreased superhelical density of circular DNA molecules. These results suggest that the sin4 delta mutation may alter the structure of chromatin, and these changes in chromatin structure may affect transcriptional regulation.

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• Dobi, K. C., Winston, F. (2007). Analysis of Transcriptional Activation at a Distance in Saccharomyces cerevisiae. Mol. Cell. Biol. 27: 5575-5586 [Abstract] [Full Text]  
• Romero, C., Desai, P., DeLillo, N., Vancura, A. (2006). Expression of FLR1 Transporter Requires Phospholipase C and Is Repressed by Mediator. J. Biol. Chem. 281: 5677-5685 [Abstract] [Full Text]  
• Beve, J., Hu, G.-Z., Myers, L. C., Balciunas, D., Werngren, O., Hultenby, K., Wibom, R., Ronne, H., Gustafsson, C. M. (2005). The Structural and Functional Role of Med5 in the Yeast Mediator Tail Module. J. Biol. Chem. 280: 41366-41372 [Abstract] [Full Text]  
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• Wang, X., Michels, C. A. (2004). Mutations in SIN4 and RGR1 Cause Constitutive Expression of MAL Structural Genes in Saccharomyces cerevisiae. Genetics 168: 747-757 [Abstract] [Full Text]  
• Guidi, B. W., Bjornsdottir, G., Hopkins, D. C., Lacomis, L., Erdjument-Bromage, H., Tempst, P., Myers, L. C. (2004). Mutual Targeting of Mediator and the TFIIH Kinase Kin28. J. Biol. Chem. 279: 29114-29120 [Abstract] [Full Text]  
• Mukai, Y., Davie, J. K., Dent, S. Y. R. (2003). Physical and Functional Interaction of the Yeast Corepressor Tup1 with mRNA 5'-Triphosphatase. J. Biol. Chem. 278: 18895-18901 [Abstract] [Full Text]  
• Park, J. M., Kim, J. M., Kim, L. K., Kim, S. N., Kim-Ha, J., Kim, J. H., Kim, Y.-J. (2003). Signal-Induced Transcriptional Activation by Dif Requires the dTRAP80 Mediator Module. Mol. Cell. Biol. 23: 1358-1367 [Abstract] [Full Text]  
• Balciunas, D., Hallberg, M., Bjorklund, S., Ronne, H. (2003). Functional Interactions within Yeast Mediator and Evidence of Differential Subunit Modifications. J. Biol. Chem. 278: 3831-3839 [Abstract] [Full Text]  
• Spencer, J. V., Arndt, K. M. (2002). A TATA Binding Protein Mutant with Increased Affinity for DNA Directs Transcription from a Reversed TATA Sequence In Vivo. Mol. Cell. Biol. 22: 8744-8755 [Abstract] [Full Text]  
• Traven, A., Staresincic, L., Arneric, M., Sopta, M. (2002). The yeast protein Xtc1 functions as a direct transcriptional repressor. Nucleic Acids Res 30: 2358-2364 [Abstract] [Full Text]  
• Blumental-Perry, A., Li, W., Simchen, G., Mitchell, A. P. (2002). Repression and Activation Domains of Rme1p Structurally Overlap, but Differ in Genetic Requirements. Mol. Biol. Cell 13: 1709-1721 [Abstract] [Full Text]  
• Kang, J. S., Kim, S. H., Hwang, M. S., Han, S. J., Lee, Y. C., Kim, Y.-J. (2001). The Structural and Functional Organization of the Yeast Mediator Complex. J. Biol. Chem. 276: 42003-42010 [Abstract] [Full Text]  
• Bhoite, L. T., Yu, Y., Stillman, D. J. (2001). The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II. Genes Dev. 15: 2457-2469 [Abstract] [Full Text]  
• Howard, S. C., Chang, Y.-W., Budovskaya, Y. V., Herman, P. K. (2001). The Ras/PKA Signaling Pathway of Saccharomyces cerevisiae Exhibits a Functional Interaction With the Sin4p Complex of the RNA Polymerase II Holoenzyme. Genetics 159: 77-89 [Abstract] [Full Text]  
• Park, J. M., Kim, H.-S., Han, S. J., Hwang, M.-S., Lee, Y. C., Kim, Y.-J. (2000). In Vivo Requirement of Activator-Specific Binding Targets of Mediator. Mol. Cell. Biol. 20: 8709-8719 [Abstract] [Full Text]  
• Watson, A. D., Edmondson, D. G., Bone, J. R., Mukai, Y., Yu, Y., Du, W., Stillman, D. J., Roth, S. Y. (2000). Ssn6-Tup1 interacts with class I histone deacetylases required for repression. Genes Dev. 14: 2737-2744 [Abstract] [Full Text]  
• Yao, S., Neiman, A., Prelich, G. (2000). BUR1 and BUR2 Encode a Divergent Cyclin-Dependent Kinase-Cyclin Complex Important for Transcription In Vivo. Mol. Cell. Biol. 20: 7080-7087 [Abstract] [Full Text]  
• Kuchin, S., Treich, I., Carlson, M. (2000). A regulatory shortcut between the Snf1 protein kinase and RNA polymerase II holoenzyme. Proc. Natl. Acad. Sci. USA 10.1073/pnas.140109897v1 [Abstract] [Full Text]  
• Dorris, D. R., Struhl, K. (2000). Artificial Recruitment of TFIID, but Not RNA Polymerase II Holoenzyme, Activates Transcription in Mammalian Cells. Mol. Cell. Biol. 20: 4350-4358 [Abstract] [Full Text]  
• Stargell, L. A., Moqtaderi, Z., Dorris, D. R., Ogg, R. C., Struhl, K. (2000). TFIIA Has Activator-dependent and Core Promoter Functions in Vivo. J. Biol. Chem. 275: 12374-12380 [Abstract] [Full Text]  
• Yu, Y., Eriksson, P., Stillman, D. J. (2000). Architectural Transcription Factors and the SAGA Complex Function in Parallel Pathways To Activate Transcription. Mol. Cell. Biol. 20: 2350-2357 [Abstract] [Full Text]  
• Kim, S., Cabane, K., Hampsey, M., Reinberg, D. (2000). Genetic Analysis of the Ydr1-Bur6 Repressor Complex Reveals an Intricate Balance among Transcriptional Regulatory Proteins in Yeast. Mol. Cell. Biol. 20: 2455-2465 [Abstract] [Full Text]  
• Mukai, Y., Matsuo, E., Roth, S. Y., Harashima, S. (1999). Conservation of Histone Binding and Transcriptional Repressor Functions in a Schizosaccharomyces pombe Tup1p Homolog. Mol. Cell. Biol. 19: 8461-8468 [Abstract] [Full Text]  
• Harata, M., Oma, Y., Mizuno, S., Jiang, Y. W., Stillman, D. J., Wintersberger, U. (1999). The Nuclear Actin-related Protein of Saccharomyces cerevisiae, Act3p/Arp4, Interacts with Core Histones. Mol. Biol. Cell 10: 2595-2605 [Abstract] [Full Text]  
• Davies, J. P., Yildiz, F. H., Grossman, A. R. (1999). Sac3, an Snf1-like Serine/Threonine Kinase That Positively and Negatively Regulates the Responses of Chlamydomonas to Sulfur Limitation. Plant Cell 11: 1179-1190 [Abstract] [Full Text]  
• Dudley, A. M., Gansheroff, L. J., Winston, F. (1999). Specific Components of the SAGA Complex Are Required for Gcn4- and Gcr1-Mediated Activation of the his4-912{delta} Promoter in Saccharomyces cerevisiae. Genetics 151: 1365-1378 [Abstract] [Full Text]  
• Gaudreau, L., Keaveney, M., Nevado, J., Zaman, Z., Bryant, G. O., Struhl, K., Ptashne, M. (1999). Transcriptional activation by artificial recruitment in yeast is influenced by promoter architecture and downstream sequences. Proc. Natl. Acad. Sci. USA 96: 2668-2673 [Abstract] [Full Text]  
• Chang, M., French-Cornay, D., Fan, H.-y., Klein, H., Denis, C. L., Jaehning, J. A. (1999). A Complex Containing RNA Polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p Plays a Role in Protein Kinase C Signaling. Mol. Cell. Biol. 19: 1056-1067 [Abstract] [Full Text]  
• Myers, L. C., Gustafsson, C. M., Hayashibara, K. C., Brown, P. O., Kornberg, R. D. (1999). Mediator protein mutations that selectively abolish activated transcription. Proc. Natl. Acad. Sci. USA 96: 67-72 [Abstract] [Full Text]  
• Myer, V. E., Young, R. A. (1998). RNA Polymerase II Holoenzymes and Subcomplexes. J. Biol. Chem. 273: 27757-27760 [Full Text]  
• Lee, Y. C., Kim, Y.-J. (1998). Requirement for a Functional Interaction between Mediator Components Med6 and Srb4 in RNA Polymerase II Transcription. Mol. Cell. Biol. 18: 5364-5370 [Abstract] [Full Text]  
• Friesen, H., Tanny, J. C., Segall, J. (1998). SPE3, Which Encodes Spermidine Synthase, Is Required for Full Repression Through NREDIT in Saccharomyces cerevisiae. Genetics 150: 59-73 [Abstract] [Full Text]  
• Tabtiang, R. K., Herskowitz, I. (1998). Nuclear Proteins Nut1p and Nut2p Cooperate To Negatively Regulate a Swi4p-Dependent lacZ Reporter Gene in Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 4707-4718 [Abstract] [Full Text]  
• Jiang, Y. W., Veschambre, P., Erdjument-Bromage, H., Tempst, P., Conaway, J. W., Conaway, R. C., Kornberg, R. D. (1998). Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. Proc. Natl. Acad. Sci. USA 95: 8538-8543 [Abstract] [Full Text]  
• Gancedo, J. M. (1998). Yeast Carbon Catabolite Repression. Microbiol. Mol. Biol. Rev. 62: 334-361 [Abstract] [Full Text]  
• Hampsey, M. (1998). Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery. Microbiol. Mol. Biol. Rev. 62: 465-503 [Abstract] [Full Text]  
• Kuchin, S., Carlson, M. (1998). Functional Relationships of Srb10-Srb11 Kinase, Carboxy-Terminal Domain Kinase CTDK-I, and Transcriptional Corepressor Ssn6-Tup1. Mol. Cell. Biol. 18: 1163-1171 [Abstract] [Full Text]  
• DeSilva, H., Lee, K., Osley, M. A. (1998). Functional Dissection of Yeast Hir1p, a WD Repeat–Containing Transcriptional Corepressor. Genetics 148: 657-668 [Abstract] [Full Text]  
• Shimizu, M., Li, W., Shindo, H., Mitchell, A. P. (1997). Transcriptional repression at a distance through exclusion of activator binding in vivo. Proc. Natl. Acad. Sci. USA 94: 790-795 [Abstract] [Full Text]  
• Gustafsson, C. M., Myers, L. C., Li, Y., Redd, M. J., Lui, M., Erdjument-Bromage, H., Tempst, P., Kornberg, R. D. (1997). Identification of Rox3 as a Component of Mediator and RNA Polymerase II Holoenzyme. J. Biol. Chem. 272: 48-50 [Abstract] [Full Text]  
• Jiang, Y W, Stillman, D J (1996). Epigenetic effects on yeast transcription caused by mutations in an actin-related protein present in the nucleus.. Genes Dev. 10: 604-619 [Abstract]  
• Cooper, J P, Roth, S Y, Simpson, R T (1994). The global transcriptional regulators, SSN6 and TUP1, play distinct roles in the establishment of a repressive chromatin structure.. Genes Dev. 8: 1400-1410 [Abstract]  
• Kokubo, T, Gong, D W, Yamashita, S, Horikoshi, M, Roeder, R G, Nakatani, Y (1993). Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID.. Genes Dev. 7: 1033-1046 [Abstract]  
• Sakurai, H., Fukasawa, T. (2000). Functional Connections between Mediator Components and General Transcription Factors of Saccharomyces cerevisiae. J. Biol. Chem. 275: 37251-37256 [Abstract] [Full Text]  
• Bone, J. R., Roth, S. Y. (2001). Recruitment of the Yeast Tup1p-Ssn6p Repressor Is Associated with Localized Decreases in Histone Acetylation. J. Biol. Chem. 276: 1808-1813 [Abstract] [Full Text]  
• Han, S. J., Lee, J.-S., Kang, J. S., Kim, Y.-J. (2001). Med9/Cse2 and Gal11 Modules Are Required for Transcriptional Repression of Distinct Group of Genes. J. Biol. Chem. 276: 37020-37026 [Abstract] [Full Text]  
• Kuchin, S., Treich, I., Carlson, M. (2000). A regulatory shortcut between the Snf1 protein kinase and RNA polymerase II holoenzyme. Proc. Natl. Acad. Sci. USA 97: 7916-7920 [Abstract] [Full Text]