This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ruggieri, R
Right arrow Articles by Matsumoto, K
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruggieri, R
Right arrow Articles by Matsumoto, K

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 February; 12(2): 758-766

RSR1, a ras-like gene homologous to Krev-1 (smg21A/rap1A): role in the development of cell polarity and interactions with the Ras pathway in Saccharomyces cerevisiae.

R Ruggieri, A Bender, Y Matsui, S Powers, Y Takai, J R Pringle and K Matsumoto

DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304.

ABSTRACT

The Saccharomyces cerevisiae ras-like gene RSR1 is particularly closely related to the mammalian gene Krev-1 (also known as smg21A and rap1A). RSR1 was originally isolated as a multicopy suppressor of a cdc24 mutation, which causes an inability to bud or establish cell polarity. Deletion of RSR1 itself does not affect growth but causes a randomization of bud position. We have now constructed mutant alleles of RSR1 encoding proteins with substitutions of Val for Gly at position 12 (analogous to constitutively activated Ras proteins) or Asn for Lys at position 16 (analogous to a dominant-negative Ras protein). rsr1Val-12 could not restore a normal budding pattern to an rsr1 deletion strain but could suppress a cdc24 mutation when overexpressed. rsr1Asn-16 could randomize the budding pattern of a wild-type strain even in low copy number but was not lethal even in high copy number. These and other results suggest that Rsr1p functions only in bud site selection and not in subsequent events of polarity establishment and bud formation, that this function involves a cycling between GTP-bound and GDP-bound forms of the protein, and that the suppression of cdc24 involves direct interaction between Rsr1p[GTP] and Cdc24p. Functional homology between Rsr1p and Krev-1 p21 was suggested by the observations that expression of the latter protein in yeast cells could both suppress a cdc24 mutation and randomize the budding pattern of wild-type cells. As Krev-1 overexpression can suppress ras-induced transformation of mammalian cells, we looked for effects of RSR1 on the S. cerevisiae Ras pathway. Although no suppression of the activated RAS2Val-19 allele was observed, overexpression of rsr1Val-12 suppressed the lethality of strains lacking RAS gene function, apparently through a direct activation of adenyl cyclase. This interaction of Rsr1p with the effector of Ras in S. cerevisiae suggests that Krev-1 may revert ras-induced transformation of mammalian cells by affecting the interaction of ras p21 with its effector.

Mol Cell Biol. 1992 February; 12(2): 758-766




This article has been cited by other articles:

  • Slaughter, B. D., Smith, S. E., Li, R. (2009). Symmetry Breaking in the Life Cycle of the Budding Yeast. Cold Spring Harb. Perspect. Biol. 1: a003384-a003384 [Abstract] [Full Text]  
  • Brand, A., Vacharaksa, A., Bendel, C., Norton, J., Haynes, P., Henry-Stanley, M., Wells, C., Ross, K., Gow, N. A. R., Gale, C. A. (2008). An Internal Polarity Landmark Is Important for Externally Induced Hyphal Behaviors in Candida albicans. Eukaryot Cell 7: 712-720 [Abstract] [Full Text]  
  • Harispe, L., Portela, C., Scazzocchio, C., Penalva, M. A., Gorfinkiel, L. (2008). Ras GTPase-Activating Protein Regulation of Actin Cytoskeleton and Hyphal Polarity in Aspergillus nidulans. Eukaryot Cell 7: 141-153 [Abstract] [Full Text]  
  • Gao, X.-D., Sperber, L. M., Kane, S. A., Tong, Z., Tong, A. H. Y., Boone, C., Bi, E. (2007). Sequential and Distinct Roles of the Cadherin Domain-containing Protein Axl2p in Cell Polarization in Yeast Cell Cycle. Mol. Biol. Cell 18: 2542-2560 [Abstract] [Full Text]  
  • Park, H.-O., Bi, E. (2007). Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond. Microbiol. Mol. Biol. Rev. 71: 48-96 [Abstract] [Full Text]  
  • Hausauer, D. L., Gerami-Nejad, M., Kistler-Anderson, C., Gale, C. A. (2005). Hyphal Guidance and Invasive Growth in Candida albicans Require the Ras-Like GTPase Rsr1p and Its GTPase-Activating Protein Bud2p. Eukaryot Cell 4: 1273-1286 [Abstract] [Full Text]  
  • Wang, Y., Chen, W., Simpson, D. M., Elion, E. A. (2005). Cdc24 Regulates Nuclear Shuttling and Recruitment of the Ste5 Scaffold to a Heterotrimeric G Protein in Saccharomyces cerevisiae. J. Biol. Chem. 280: 13084-13096 [Abstract] [Full Text]  
  • Fukuyama, T., Ogita, H., Kawakatsu, T., Fukuhara, T., Yamada, T., Sato, T., Shimizu, K., Nakamura, T., Matsuda, M., Takai, Y. (2005). Involvement of the c-Src-Crk-C3G-Rap1 Signaling in the Nectin-induced Activation of Cdc42 and Formation of Adherens Junctions. J. Biol. Chem. 280: 815-825 [Abstract] [Full Text]  
  • Hogan, C., Serpente, N., Cogram, P., Hosking, C. R., Bialucha, C. U., Feller, S. M., Braga, V. M. M., Birchmeier, W., Fujita, Y. (2004). Rap1 Regulates the Formation of E-Cadherin-Based Cell-Cell Contacts. Mol. Cell. Biol. 24: 6690-6700 [Abstract] [Full Text]  
  • Kozminski, K. G., Beven, L., Angerman, E., Tong, A. H. Y., Boone, C., Park, H.-O. (2003). Interaction between a Ras and a Rho GTPase Couples Selection of a Growth Site to the Development of Cell Polarity in Yeast. Mol. Biol. Cell 14: 4958-4970 [Abstract] [Full Text]  
  • Marchuk, D. A., Srinivasan, S., Squire, T. L., Zawistowski, J. S. (2003). Vascular morphogenesis: tales of two syndromes. Hum Mol Genet 12: R97-112 [Abstract] [Full Text]  
  • Cullen, P. J., Sprague, G. F. Jr. (2002). The Roles of Bud-Site-Selection Proteins during Haploid Invasive Growth in Yeast. Mol. Biol. Cell 13: 2990-3004 [Abstract] [Full Text]  
  • Gunel, M., Laurans, M. S. H., Shin, D., DiLuna, M. L., Voorhees, J., Choate, K., Nelson-Williams, C., Lifton, R. P. (2002). KRIT1, a gene mutated in cerebral cavernous malformation, encodes a microtubule-associated protein. Proc. Natl. Acad. Sci. USA 99: 10677-10682 [Abstract] [Full Text]  
  • Park, H.-O., Kang, P. J., Rachfal, A. W. (2002). Localization of the Rsr1/Bud1 GTPase Involved in Selection of a Proper Growth Site in Yeast. J. Biol. Chem. 277: 26721-26724 [Abstract] [Full Text]  
  • Drees, B. L., Sundin, B., Brazeau, E., Caviston, J. P., Chen, G.-C., Guo, W., Kozminski, K. G., Lau, M. W., Moskow, J. J., Tong, A., Schenkman, L. R., McKenzie, A. III, Brennwald, P., Longtine, M., Bi, E., Chan, C., Novick, P., Boone, C., Pringle, J. R., Davis, T. N., Fields, S., Drubin, D. G. (2001). A protein interaction map for cell polarity development. JCB 154: 549-576 [Abstract] [Full Text]  
  • Ni, L., Snyder, M. (2001). A Genomic Study of the Bipolar Bud Site Selection Pattern in Saccharomyces cerevisiae. Mol. Biol. Cell 12: 2147-2170 [Abstract] [Full Text]  
  • Gulli, M.-P., Peter, M. (2001). Temporal and spatial regulation of Rho-type guanine-nucleotide exchange factors: the yeast perspective. Genes Dev. 15: 365-379 [Full Text]  
  • Freedman, T., Porter, A., Haarer, B. (2000). Mutational and hyperexpression-induced disruption of bipolar budding in yeast. Microbiology 146: 2833-2843 [Abstract] [Full Text]  
  • EINARSON, M. B., GOLEMIS, E. A. (2000). Encroaching genomics: adapting large-scale science to small academic laboratories. Physiol. Genomics 2: 85-92 [Abstract] [Full Text]  
  • Johnson, D. I. (1999). Cdc42: An Essential Rho-Type GTPase Controlling Eukaryotic Cell Polarity. Microbiol. Mol. Biol. Rev. 63: 54-105 [Abstract] [Full Text]  
  • Davis, C. R., Richman, T. J., Deliduka, S. B., Blaisdell, J. O., Collins, C. C., Johnson, D. I. (1998). Analysis of the Mechanisms of Action of the Saccharomyces cerevisiae Dominant Lethal cdc42G12V and Dominant Negative cdc42D118A Mutations. J. Biol. Chem. 273: 849-858 [Abstract] [Full Text]  
  • Chen, G.-C., Kim, Y.-J., Chan, C. S.M. (1997). The Cdc42 GTPase-associated proteins Gic1 and Gic2 are required for polarized cell growth in Saccharomyces cerevisiae. Genes Dev. 11: 2958-2971 [Abstract] [Full Text]  
  • Park, H.-O., Bi, E., Pringle, J. R., Herskowitz, I. (1997). Two active states of the Ras-related Bud1/Rsr1 protein bind to different effectors to determine yeast cell polarity. Proc. Natl. Acad. Sci. USA 94: 4463-4468 [Abstract] [Full Text]  
  • Stevenson, B J, Ferguson, B, De Virgilio, C, Bi, E, Pringle, J R, Ammerer, G, Sprague, G F (1995). Mutation of RGA1, which encodes a putative GTPase-activating protein for the polarity-establishment protein Cdc42p, activates the pheromone-response pathway in the yeast Saccharomyces cerevisiae.. Genes Dev. 9: 2949-2963 [Abstract]  
  • Morishita, T., Mitsuzawa, H., Nakafuku, M., Nakamura, S., Hattori, S., Anraku, Y. (1995). Requirement of Saccharomyces cerevisiae Ras for Completion of Mitosis. Science 270: 1213-1215 [Abstract]  
  • Zheng, Y., Bender, A., Cerione, R. A. (1995). Interactions among Proteins Involved in Bud-site Selection and Bud-site Assembly in Saccharomyces cerevisiae. J. Biol. Chem. 270: 626-630 [Abstract] [Full Text]  
  • Herskowitz, I., Park, H.-O., Sanders, S., Valtz, N., Peter, M. (1995). Programming of Cell Polarity in Budding Yeast by Endogenous and Exogenous Signals. Cold Spring Harb Symp Quant Biol 60: 717-727 [Abstract]  
  • Pringle, J.R., Bi, E., Harkins, H.A., Zahner, J.E., De Virgilio, C., Chant, J., Corrado, K., Fares, H. (1995). Establishment of Cell Polarity in Yeast. Cold Spring Harb Symp Quant Biol 60: 729-744 [Abstract]  
  • Freed, E, Symons, M, Macdonald, S., McCormick, F, Ruggieri, R (1994). Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation. Science 265: 1713-1716 [Abstract]  
  • Herman, M., Horvitz, H. (1994). The Caenorhabditis elegans gene lin-44 controls the polarity of asymmetric cell divisions. Development 120: 1035-1047 [Abstract]  
  • Freed, E., McCormick, F., Ruggieri, R. (1994). Proteins of the 14-3-3 Family Associate with Raf and Contribute to Its Activation. Cold Spring Harb Symp Quant Biol 59: 187-193 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»