This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Goedecke, W Articles by Pfeiffer, P Search for Related Content PubMed PubMed Citation Articles by Goedecke, W Articles by Pfeiffer, P

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 February; 12(2): 811-816

Activation of a system for the joining of nonhomologous DNA ends during Xenopus egg maturation.

Institute for Genetics, University of Cologne, Germany.

ABSTRACT

Mature Xenopus laevis eggs provide an elementary reaction system of illegitimate recombination which efficiently joins nonhomologous DNA ends (P. Pfeiffer and W. Vielmetter, Nucleic Acids Res. 16:907-924, 1988). Here we show that stage VI oocytes, known to express a system for homologous recombination (D. Carroll, Proc. Natl. Acad. Sci. USA 80:6902-6906, 1983), are completely devoid of this joining system. Nonhomologous DNA end-to-end joining, however, attains full activity only at an extremely late stage of egg maturation. Cycloheximide inhibition patterns indicate that nonhomologous joining activity is regulated at the G2 restriction point of the cell cycle. Implications of homologous and nonhomologous recombination activities during egg maturation are discussed.

This article has been cited by other articles:

• Manivasakam, P., Aubrecht, J., Sidhom, S., Schiestl, R. H. (2001). Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells. Nucleic Acids Res 29: 4826-4833 [Abstract] [Full Text]  
• Moore, C. W., McKoy, J., Dardalhon, M., Davermann, D., Martinez, M., Averbeck, D. (2000). DNA Damage-Inducible and RAD52-Independent Repair of DNA Double-Strand Breaks in Saccharomyces cerevisiae. Genetics 154: 1085-1099 [Abstract] [Full Text]  
• Labhart, P. (1999). Ku-Dependent Nonhomologous DNA End Joining in Xenopus Egg Extracts. Mol. Cell. Biol. 19: 2585-2593 [Abstract] [Full Text]  
• Gu, X.-Y., Bennett, R. A.O., Povirk, L. F. (1996). End-joining of Free Radical-mediated DNA Double-strand Breaks in Vitro Is Blocked by the Kinase Inhibitor Wortmannin at a Step Preceding Removal of Damaged 3' Termini. J. Biol. Chem. 271: 19660-19663 [Abstract] [Full Text]