This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lim, L C Articles by Sheffery, M Search for Related Content PubMed PubMed Citation Articles by Lim, L C Articles by Sheffery, M

Molecular cloning of the alpha-globin transcription factor CP2.

DeWitt Wallace Research Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

ABSTRACT

CP2, a transcription factor that binds the murine alpha-globin promoter, was purified and subjected to amino acid sequence analysis. Oligonucleotide primers derived from the sequence were used to obtain murine and human cDNA clones for the factor. The murine cDNA spans approximately 4 kb and contains two coextensive open reading frames (ORFs) which encode deduced polypeptides of 529 (ORF-1; molecular weight, 59,802) and 502 (ORF-2; molecular weight, 56,957) amino acids, slightly smaller than the purified factor as estimated from its mobility in sodium dodecyl sulfate-polyacrylamide gels (64,000 to 66,000). The human cDNA contains a single ORF of 501 amino acids that is nearly contiguous with murine ORF-2. Indeed, comparison of deduced human and murine amino acid sequences shows that the two polypeptides are 96.4% identical. A strictly conserved region is rich in serine and threonine (17.5%) and in proline (11%) residues (S-T-P domain). This S-T-P domain is immediately amino terminal to a string of 10 glutamines (in the human sequence) or a tract of alternating glutamine and proline residues (in the mouse sequence). Bacterial expression of the full-length (502-amino-acid) murine factor or of a core region comprising amino acids 133 to 395 generated polypeptides with the DNA binding specificity of CP2. These results confirmed the cloning of CP2 and delimited the region sufficient for specific DNA sequence recognition. Antisera produced against the core region recognized polypeptide species with Mrs of 64,000 and 66,000 in immune blots of nuclear extracts prepared from both murine and human cell lines, consistent with the size of the purified factor. Lastly, a data base search revealed that amino acids 63 to 270 of the murine factor are distantly related to a domain in the Drosophila gene regulatory factor Elf-1.

This article has been cited by other articles:

• Yamaguchi, Y., Yonemura, S., Takada, S. (2006). Grainyhead-related transcription factor is required for duct maturation in the salivary gland and the kidney of the mouse. Development 133: 4737-4748 [Abstract] [Full Text]  
• Bose, F., Fugazza, C., Casalgrandi, M., Capelli, A., Cunningham, J. M., Zhao, Q., Jane, S. M., Ottolenghi, S., Ronchi, A. (2006). Functional Interaction of CP2 with GATA-1 in the Regulation of Erythroid Promoters. Mol. Cell. Biol. 26: 3942-3954 [Abstract] [Full Text]  
• Sato, F., Yasumoto, K.-i., Kimura, K., Numayama-Tsuruta, K., Sogawa, K. (2005). Heterodimerization with LBP-1b is necessary for nuclear localization of LBP-1a and LBP-1c. GENES CELLS 10: 861-870 [Abstract] [Full Text]  
• Kang, H. C., Chae, J. H., Lee, Y. H., Park, M.-A., Shin, J. H., Kim, S.-H., Ye, S.-K., Cho, Y. S., Fiering, S., Kim, C. G. (2005). Erythroid Cell-Specific {alpha}-Globin Gene Regulation by the CP2 Transcription Factor Family. Mol. Cell. Biol. 25: 6005-6020 [Abstract] [Full Text]  
• Ylisastigui, L., Kaur, R., Johnson, H., Volker, J., He, G., Hansen, U., Margolis, D. (2005). Mitogen-Activated Protein Kinases Regulate LSF Occupancy at the Human Immunodeficiency Virus Type 1 Promoter. J. Virol. 79: 5952-5962 [Abstract] [Full Text]  
• Huang, N., Miller, W. L. (2005). LBP Proteins Modulate SF1-Independent Expression of P450scc in Human Placental JEG-3 Cells. Mol. Endocrinol. 19: 409-420 [Abstract] [Full Text]  
• Parekh, V., McEwen, A., Barbour, V., Takahashi, Y., Rehg, J. E., Jane, S. M., Cunningham, J. M. (2004). Defective Extraembryonic Angiogenesis in Mice Lacking LBP-1a, a Member of the grainyhead Family of Transcription Factors. Mol. Cell. Biol. 24: 7113-7129 [Abstract] [Full Text]  
• Acloque, H., Mey, A., Birot, A. M., Gruffat, H., Pain, B., Samarut, J. (2004). Transcription factor cCP2 controls gene expression in chicken embryonic stem cells. Nucleic Acids Res 32: 2259-2271 [Abstract] [Full Text]  
• Venkatesan, K., McManus, H. R., Mello, C. C., Smith, T. F., Hansen, U. (2003). Functional conservation between members of an ancient duplicated transcription factor family, LSF/Grainyhead. Nucleic Acids Res 31: 4304-4316 [Abstract] [Full Text]  
• Bruni, P., Minopoli, G., Brancaccio, T., Napolitano, M., Faraonio, R., Zambrano, N., Hansen, U., Russo, T. (2002). Fe65, a Ligand of the Alzheimer's beta -Amyloid Precursor Protein, Blocks Cell Cycle Progression by Down-regulating Thymidylate Synthase Expression. J. Biol. Chem. 277: 35481-35488 [Abstract] [Full Text]  
• Frith, M. C., Spouge, J. L., Hansen, U., Weng, Z. (2002). Statistical significance of clusters of motifs represented by position specific scoring matrices in nucleotide sequences. Nucleic Acids Res 30: 3214-3224 [Abstract] [Full Text]  
• Tuckfield, A., Clouston, D. R., Wilanowski, T. M., Zhao, L.-L., Cunningham, J. M., Jane, S. M. (2002). Binding of the RING Polycomb Proteins to Specific Target Genes in Complex with the grainyhead-Like Family of Developmental Transcription Factors. Mol. Cell. Biol. 22: 1936-1946 [Abstract] [Full Text]  
• Drouin, E. E., Schrader, C. E., Stavnezer, J., Hansen, U. (2002). The Ubiquitously Expressed DNA-Binding Protein Late SV40 Factor Binds Ig Switch Regions and Represses Class Switching to IgA. J. Immunol. 168: 2847-2856 [Abstract] [Full Text]  
• Zheng, J. B., Zhou, Y.-H., Maity, T., Liao, W. S.-L., Saunders, G. F. (2001). Activation of the human PAX6 gene through the exon 1 enhancer by transcription factors SEF and Sp1. Nucleic Acids Res 29: 4070-4078 [Abstract] [Full Text]  
• Zhou, W., Clouston, D. R., Wang, X., Cerruti, L., Cunningham, J. M., Jane, S. M. (2000). Induction of Human Fetal Globin Gene Expression by a Novel Erythroid Factor, NF-E4. Mol. Cell. Biol. 20: 7662-7672 [Abstract] [Full Text]  
• Lambert, J.-C., Goumidi, L., Vrieze, F. W.-D., Frigard, B., Harris, J. M., Cummings, A., Coates, J., Pasquier, F., Cottel, D., Gaillac, M., St Clair, D., Mann, D. M.A., Hardy, J., Lendon, C. L., Amouyel, P., Chartier-Harlin, M.-C. (2000). The transcriptional factor LBP-1c/CP2/LSF gene on chromosome 12 is a genetic determinant of Alzheimer's disease. Hum Mol Genet 9: 2275-2280 [Abstract] [Full Text]  
• Coull, J. J., Romerio, F., Sun, J.-M., Volker, J. L., Galvin, K. M., Davie, J. R., Shi, Y., Hansen, U., Margolis, D. M. (2000). The Human Factors YY1 and LSF Repress the Human Immunodeficiency Virus Type 1 Long Terminal Repeat via Recruitment of Histone Deacetylase 1. J. Virol. 74: 6790-6799 [Abstract] [Full Text]  
• Huang, N., Miller, W. L. (2000). Cloning of Factors Related to HIV-inducible LBP Proteins That Regulate Steroidogenic Factor-1-independent Human Placental Transcription of the Cholesterol Side-chain Cleavage Enzyme, P450scc. J. Biol. Chem. 275: 2852-2858 [Abstract] [Full Text]  
• Hayashi, Y., Yamagishi, M., Nishimoto, Y., Taguchi, O., Matsukage, A., Yamaguchi, M. (1999). A Binding Site for the Transcription Factor Grainyhead/Nuclear Transcription Factor-1 Contributes to Regulation of the Drosophila Proliferating Cell Nuclear Antigen Gene Promoter. J. Biol. Chem. 274: 35080-35088 [Abstract] [Full Text]  
• Bing, Z., Reddy, S. A. G., Ren, Y., Qin, J., Liao, W. S.-L. (1999). Purification and Characterization of the Serum Amyloid A3 Enhancer Factor. J. Biol. Chem. 274: 24649-24656 [Abstract] [Full Text]  
• Chen, X., Reitman, M., Bieker, J. J. (1998). Chromatin Structure and Transcriptional Control Elements of the Erythroid Kruppel-like Factor (EKLF) Gene. J. Biol. Chem. 273: 25031-25040 [Abstract] [Full Text]  
• Zambrano, N., Minopoli, G., de Candia, P., Russo, T. (1998). The Fe65 Adaptor Protein Interacts through Its PID1 Domain with the Transcription Factor CP2/LSF/LBP1. J. Biol. Chem. 273: 20128-20133 [Abstract] [Full Text]  
• Shirra, M. K., Hansen, U. (1998). LSF and NTF-1 Share a Conserved DNA Recognition Motif yet Require Different Oligomerization States to Form a Stable Protein-DNA Complex. J. Biol. Chem. 273: 19260-19268 [Abstract] [Full Text]  
• Sheridan, P. L., Mayall, T. P., Verdin, E., Jones, K. A. (1997). Histone acetyltransferases regulate HIV-1 enhancer activity in vitro. Genes Dev. 11: 3327-3340 [Abstract] [Full Text]  
• Volker, J L, Rameh, L E, Zhu, Q, DeCaprio, J, Hansen, U (1997). Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity.. Genes Dev. 11: 1435-1446 [Abstract]  
• Alonso, C. R., Pesce, C. G., Kornblihtt, A. R. (1996). The CCAAT-binding Proteins CP1 and NF-I Cooperate with ATF-2 in the Transcription of the Fibronectin Gene. J. Biol. Chem. 271: 22271-22279 [Abstract] [Full Text]  
• Attardi, L D, Tjian, R (1993). Drosophila tissue-specific transcription factor NTF-1 contains a novel isoleucine-rich activation motif.. Genes Dev. 7: 1341-1353 [Abstract]  
• Ramamurthy, L., Barbour, V., Tuckfield, A., Clouston, D. R., Topham, D., Cunningham, J. M., Jane, S. M. (2001). Targeted Disruption of the CP2 Gene, a Member of the NTF Family of Transcription Factors. J. Biol. Chem. 276: 7836-7842 [Abstract] [Full Text]  
• Bing, Z., Huang, J. H., Liao, W. S.-L. (2000). NFkappa B Interacts with Serum Amyloid A3 Enhancer Factor to Synergistically Activate Mouse Serum Amyloid A3 Gene Transcription. J. Biol. Chem. 275: 31616-31623 [Abstract] [Full Text]  
• Casolaro, V., Keane-Myers, A. M., Swendeman, S. L., Steindler, C., Zhong, F., Sheffery, M., Georas, S. N., Ono, S. J. (2000). Identification and Characterization of a Critical CP2-binding Element in the Human Interleukin-4 Promoter. J. Biol. Chem. 275: 36605-36611 [Abstract] [Full Text]  
• Rodda, S., Sharma, S., Scherer, M., Chapman, G., Rathjen, P. (2001). CRTR-1, a Developmentally Regulated Transcriptional Repressor Related to the CP2 Family of Transcription Factors. J. Biol. Chem. 276: 3324-3332 [Abstract] [Full Text]