This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Masson, N Articles by Lee, K A Search for Related Content PubMed PubMed Citation Articles by Masson, N Articles by Lee, K A

Cyclic AMP response element-binding protein and the catalytic subunit of protein kinase A are present in F9 embryonal carcinoma cells but are unable to activate the somatostatin promoter.

Gene Activation Laboratory, Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, United Kingdom.

ABSTRACT

The cyclic AMP (cAMP) response elements (CREs) of the somatostatin and vasoactive intestinal peptide (VIP) promoters contain binding sites for CRE-binding protein (CREB) that are essential for cAMP-regulated transcription. Using F9 embryonal carcinoma cells, we show that the somatostatin and VIP promoters exhibit a differentiation-dependent cAMP response, demonstrating that these promoters are regulated by transcription factors that become active during differentiation. Lack of cAMP responsiveness of the somatostatin promoter in undifferentiated cells is not due to the absence of known positive-acting factors (the catalytic subunit of protein kinase A [cPKA] and CREB) or a general inhibition of protein kinase A activity. Since overexpression of exogenous cPKA and CREB is sufficient to activate the somatostatin promoter in undifferentiated cells, these findings suggest that a negative factor(s) represses endogenous cPKA and CREB. In contrast to their effects on somatostatin, exogenous CREB and cPKA do not activate the VIP promoter. Thus, despite coregulation during differentiation and the ability to bind CREB, the somatostatin and VIP promoters are not coordinately activated by CREB in undifferentiated F9 cells.

This article has been cited by other articles:

• Hagmaier, K., Stock, N., Precious, B., Childs, K., Wang, L.-F., Goodbourn, S., Randall, R. E. (2007). Mapuera virus, a rubulavirus that inhibits interferon signalling in a wide variety of mammalian cells without degrading STATs. J. Gen. Virol. 88: 956-966 [Abstract] [Full Text]  
• Sherwood, V., Burgert, H.-G., Chen, Y.-H., Sanghera, S., Katafigiotis, S., Randall, R. E., Connerton, I., Mellits, K. H. (2007). Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation. J. Gen. Virol. 88: 71-76 [Abstract] [Full Text]  
• Hilton, L., Moganeradj, K., Zhang, G., Chen, Y.-H., Randall, R. E., McCauley, J. W., Goodbourn, S. (2006). The NPro Product of Bovine Viral Diarrhea Virus Inhibits DNA Binding by Interferon Regulatory Factor 3 and Targets It for Proteasomal Degradation. J. Virol. 80: 11723-11732 [Abstract] [Full Text]  
• Childs, K. S., Goodbourn, S. (2003). Identification of novel co-repressor molecules for Interferon Regulatory Factor-2. Nucleic Acids Res 31: 3016-3026 [Abstract] [Full Text]  
• Hiraiwa, N., Yabuta, T., Yoritomi, K., Hiraiwa, M., Tanaka, Y., Suzuki, T., Yoshida, M., Kannagi, R. (2003). Transactivation of the fucosyltransferase VII gene by human T-cell leukemia virus type 1 Tax through a variant cAMP-responsive element. Blood 101: 3615-3621 [Abstract] [Full Text]  
• Andrejeva, J., Young, D. F., Goodbourn, S., Randall, R. E. (2002). Degradation of STAT1 and STAT2 by the V Proteins of Simian Virus 5 and Human Parainfluenza Virus Type 2, Respectively: Consequences for Virus Replication in the Presence of Alpha/Beta and Gamma Interferons. J. Virol. 76: 2159-2167 [Abstract] [Full Text]  
• Young, D. F., Chatziandreou, N., He, B., Goodbourn, S., Lamb, R. A., Randall, R. E. (2001). Single Amino Acid Substitution in the V Protein of Simian Virus 5 Differentiates Its Ability To Block Interferon Signaling in Human and Murine Cells. J. Virol. 75: 3363-3370 [Abstract] [Full Text]  
• Cardinaux, J.-R., Notis, J. C., Zhang, Q., Vo, N., Craig, J. C., Fass, D. M., Brennan, R. G., Goodman, R. H. (2000). Recruitment of CREB Binding Protein Is Sufficient for CREB-Mediated Gene Activation. Mol. Cell. Biol. 20: 1546-1552 [Abstract] [Full Text]  
• Groussin, L., Massias, J. F., Bertagna, X., Bertherat, J. (2000). Loss of Expression of the Ubiquitous Transcription Factor cAMP Response Element-Binding Protein (CREB) and Compensatory Overexpression of the Activator CREM{tau} in the Human Adrenocortical Cancer Cell Line H295R. J. Clin. Endocrinol. Metab. 85: 345-354 [Abstract] [Full Text]  
• Didcock, L., Young, D. F., Goodbourn, S., Randall, R. E. (1999). The V Protein of Simian Virus 5 Inhibits Interferon Signalling by Targeting STAT1 for Proteasome-Mediated Degradation. J. Virol. 73: 9928-9933 [Abstract] [Full Text]  
• Kingsley-Kallesen, M. L., Kelly, D., Rizzino, A. (1999). Transcriptional Regulation of the Transforming Growth Factor-beta 2 Promoter by cAMP-responsive Element-binding Protein (CREB) and Activating Transcription Factor-1 (ATF-1) Is Modulated by Protein Kinases and the Coactivators p300 and CREB-binding Protein. J. Biol. Chem. 274: 34020-34028 [Abstract] [Full Text]  
• Didcock, L., Young, D. F., Goodbourn, S., Randall, R. E. (1999). Sendai Virus and Simian Virus 5 Block Activation of Interferon-Responsive Genes: Importance for Virus Pathogenesis. J. Virol. 73: 3125-3133 [Abstract] [Full Text]  
• Laurance, M. E., Kwok, R. P.S., Huang, M. S., Richards, J. P., Lundblad, J. R., Goodman, R. H. (1997). Differential Activation of Viral and Cellular Promoters by Human T-cell Lymphotropic Virus-1 Tax and cAMP-responsive Element Modulator Isoforms. J. Biol. Chem. 272: 2646-2651 [Abstract] [Full Text]  
• Vietor, I., Oliveira, I. C., Vilcek, J. (1996). CCAAT Box Enhancer Binding Protein alpha (C/EBP-alpha) Stimulates kappaB Element-mediated Transcription in Transfected Cells. J. Biol. Chem. 271: 5595-5602 [Abstract] [Full Text]  
• Marksitzer, R., Stief, A., Menoud, P.-A., Nagamine, Y. (1995). Role of LFB3 in Cell-specific cAMP Induction of the Urokinase-type Plasminogen Activator Gene. J. Biol. Chem. 270: 21833-21838 [Abstract] [Full Text]