This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Farber, J M Search for Related Content PubMed PubMed Citation Articles by Farber, J M

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 April; 12(4): 1535-1545

A collection of mRNA species that are inducible in the RAW 264.7 mouse macrophage cell line by gamma interferon and other agents.

Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

ABSTRACT

To identify genes induced during macrophage activation, a cDNA library was prepared from cultures of the RAW 264.7 mouse macrophage cell line that had been treated with conditioned medium from mitogen-stimulated spleen cells, and the cDNA library was screened by differential plaque hybridization. Eleven cDNA clones, designated CRG-1 through CRG-11, corresponding to mRNA species inducible in RAW 264.7 cells by the spleen cell conditioned medium, were isolated. Inductions were not blocked by cycloheximide. All of the mRNAs were inducible by gamma interferon, and some were also inducible by alpha and beta interferons, by lipopolysaccharide, by phorbol 12-myristate 13-acetate, and by the calcium ionophore A23187. Sequencing of the cDNAs revealed that CRG-1, CRG-3, and CRG-5 are cDNAs of recently identified transcription factors IRF-1, zif/268, and LRF-1 respectively. As previously reported, CRG-2 and CRG-10 (MIG) encode new members of the platelet factor 4 family of cytokines. CRG-6 corresponds to a new member of a family of interferon-inducible genes clustered on mouse chromosome 1, CRG-9 corresponds to a prostaglandin synthase homolog, CRG-8 corresponds to beta 2-microglobulin, and CRG-4 corresponds to metallothionein II. CRG-11 contains sequences of a truncated L1Md repetitive element as well as nonrepetitive sequences. The nonrepetitive sequence of CRG-11 as well as the sequences of CRG-7 are not closely related to published sequences. The CRG genes and proteins are of interest because of their involvement in macrophage activation, because of their roles as mediators of the effects of gamma interferon and other pleiotropic agents, and because of their usefulness as tools for studying the signal pathways through which gamma interferon and other inducers exert their effects on gene and protein expression.

This article has been cited by other articles:

• Whitmore, M. M., Iparraguirre, A., Kubelka, L., Weninger, W., Hai, T., Williams, B. R. G. (2007). Negative Regulation of TLR-Signaling Pathways by Activating Transcription Factor-3. J. Immunol. 179: 3622-3630 [Abstract] [Full Text]  
• Woo, A. L., Gildea, L. A., Tack, L. M., Miller, M. L., Spicer, Z., Millhorn, D. E., Finkelman, F. D., Hassett, D. J., Shull, G. E. (2002). In Vivo Evidence for Interferon-gamma -mediated Homeostatic Mechanisms in Small Intestine of the NHE3 Na+/H+ Exchanger Knockout Model of Congenital Diarrhea. J. Biol. Chem. 277: 49036-49046 [Abstract] [Full Text]  
• Park, M. K., Amichay, D., Love, P., Wick, E., Liao, F., Grinberg, A., Rabin, R. L., Zhang, H. H., Gebeyehu, S., Wright, T. M., Iwasaki, A., Weng, Y., DeMartino, J. A., Elkins, K. L., Farber, J. M. (2002). The CXC Chemokine Murine Monokine Induced by IFN-{gamma} (CXC Chemokine Ligand 9) Is Made by APCs, Targets Lymphocytes Including Activated B Cells, and Supports Antibody Responses to a Bacterial Pathogen In Vivo. J. Immunol. 169: 1433-1443 [Abstract] [Full Text]  
• Allen-Jennings, A. E., Hartman, M. G., Kociba, G. J., Hai, T. (2002). The Roles of ATF3 in Liver Dysfunction and the Regulation of Phosphoenolpyruvate Carboxykinase Gene Expression. J. Biol. Chem. 277: 20020-20025 [Abstract] [Full Text]  
• Park, M. K., Hoffmann, K. F., Cheever, A. W., Amichay, D., Wynn, T. A., Farber, J. M. (2001). Patterns of Chemokine Expression in Models of Schistosoma mansoni Inflammation and Infection Reveal Relationships between Type 1 and Type 2 Responses and Chemokines In Vivo. Infect. Immun. 69: 6755-6768 [Abstract] [Full Text]  
• Okamoto, Y., Chaves, A., Chen, J., Kelley, R., Jones, K., Weed, H. G., Gardner, K. L., Gangi, L., Yamaguchi, M., Klomkleaw, W., Nakayama, T., Hamlin, R. L., Carnes, C., Altschuld, R., Bauer, J., Hai, T. (2001). Transgenic Mice with Cardiac-Specific Expression of Activating Transcription Factor 3, a Stress-Inducible Gene, Have Conduction Abnormalities and Contractile Dysfunction. Am. J. Pathol. 159: 639-650 [Abstract] [Full Text]  
• Sauder, C., Hallensleben, W., Pagenstecher, A., Schneckenburger, S., Biro, L., Pertlik, D., Hausmann, J., Suter, M., Staeheli, P. (2000). Chemokine Gene Expression in Astrocytes of Borna Disease Virus-Infected Rats and Mice in the Absence of Inflammation. J. Virol. 74: 9267-9280 [Abstract] [Full Text]  
• Liu, M. T., Chen, B. P., Oertel, P., Buchmeier, M. J., Armstrong, D., Hamilton, T. A., Lane, T. E. (2000). Cutting Edge: The T Cell Chemoattractant IFN-Inducible Protein 10 Is Essential in Host Defense Against Viral-Induced Neurologic Disease. J. Immunol. 165: 2327-2330 [Abstract] [Full Text]  
• Belperio, J. A., Keane, M. P., Arenberg, D. A., Addison, C. L., Ehlert, J. E., Burdick, M. D., Strieter, R. M. (2000). CXC chemokines in angiogenesis. J. Leukoc. Biol. 68: 1-8 [Abstract] [Full Text]  
• Widney, D. P., Xia, Y.-R., Lusis, A. J., Smith, J. B. (2000). The Murine Chemokine CXCL11 (IFN-Inducible T Cell {alpha} Chemoattractant) Is an IFN-{gamma}- and Lipopolysaccharide- Inducible Glucocorticoid-Attenuated Response Gene Expressed in Lung and Other Tissues During Endotoxemia. J. Immunol. 164: 6322-6331 [Abstract] [Full Text]  
• Gasperini, S., Marchi, M., Calzetti, F., Laudanna, C., Vicentini, L., Olsen, H., Murphy, M., Liao, F., Farber, J., Cassatella, M. A. (1999). Gene Expression and Production of the Monokine Induced by IFN-{gamma} (MIG), IFN-Inducible T Cell {alpha} Chemoattractant (I-TAC), and IFN-{gamma}-Inducible Protein-10 (IP-10) Chemokines by Human Neutrophils. J. Immunol. 162: 4928-4937 [Abstract] [Full Text]  
• Mahalingam, S., Farber, J. M., Karupiah, G. (1999). The Interferon-Inducible Chemokines MuMig and Crg-2 Exhibit Antiviral Activity In Vivo. J. Virol. 73: 1479-1491 [Abstract] [Full Text]  
• Williams, J. A., Shacter, E. (1997). Regulation of Macrophage Cytokine Production by Prostaglandin E2. DISTINCT ROLES OF CYCLOOXYGENASE-1 AND -2. J. Biol. Chem. 272: 25693-25699 [Abstract] [Full Text]  
• Hernandez, J., Carrasco, J., Arbones, M.L., Hidalgo, J. (1997). IFN-{gamma}R -/- mice show an enhanced liver and brain metallothionein I+II response to endotoxin but not to immobilization stress. Innate Immunity 4: 363-370 [Abstract]  
• Smith, J. B., Herschman, H. R. (1995). Glucocorticoid-attenuated Response Genes Encode Intercellular Mediators, Including a New C-X-C Chemokine. J. Biol. Chem. 270: 16756-16765 [Abstract] [Full Text]  
• Allen-Jennings, A. E., Hartman, M. G., Kociba, G. J., Hai, T. (2001). The Roles of ATF3 in Glucose Homeostasis. A TRANSGENIC MOUSE MODEL WITH LIVER DYSFUNCTION AND DEFECTS IN ENDOCRINE PANCREAS. J. Biol. Chem. 276: 29507-29514 [Abstract] [Full Text]  
• Wolfgang, C. D., Liang, G., Okamoto, Y., Allen, A. E., Hai, T. (2000). Transcriptional Autorepression of the Stress-inducible Gene ATF3. J. Biol. Chem. 275: 16865-16870 [Abstract] [Full Text]