This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Keller, A D Articles by Maniatis, T Search for Related Content PubMed PubMed Citation Articles by Keller, A D Articles by Maniatis, T

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 May; 12(5): 1940-1949

Only two of the five zinc fingers of the eukaryotic transcriptional repressor PRDI-BF1 are required for sequence-specific DNA binding.

Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

ABSTRACT

The eukaryotic transcriptional repressor PRDI-BF1 contains five zinc fingers of the C2H2 type, and the protein binds specifically to PRDI, a 14-bp regulatory element of the beta interferon gene promoter. We have investigated the amino acid sequence requirements for specific binding to PRDI and found that the five zinc fingers and a short stretch of amino acids N terminal to the first finger are necessary and sufficient for PRDI-specific binding. The contribution of individual zinc fingers to DNA binding was investigated by inserting them in various combinations into another zinc finger-containing DNA-binding protein whose own fingers had been removed. We found that insertion of PRDI-BF1 zinc fingers 1 and 2 confer PRDI-binding activity on the recipient protein. In contrast, the insertion of PRDI-BF1 zinc fingers 2 through 5, the insertion of zinc finger 1 or 2 alone, and the insertion of zinc fingers 1 and 2 in reverse order did not confer PRDI-binding activity. We conclude that the first two PRDI-BF1 zinc fingers together are sufficient for the sequence-specific recognition of PRDI.

This article has been cited by other articles:

• Tsai, C.-M., Chiu, Y.-K., Hsu, T.-L., Lin, I-Y., Hsieh, S.-L., Lin, K.-I (2008). Galectin-1 Promotes Immunoglobulin Production during Plasma Cell Differentiation. J. Immunol. 181: 4570-4579 [Abstract] [Full Text]  
• Robertson, E. J., Charatsi, I., Joyner, C. J., Koonce, C. H., Morgan, M., Islam, A., Paterson, C., Lejsek, E., Arnold, S. J., Kallies, A., Nutt, S. L., Bikoff, E. K. (2007). Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice. Development 134: 4335-4345 [Abstract] [Full Text]  
• Tam, W., Gomez, M., Chadburn, A., Lee, J. W., Chan, W. C., Knowles, D. M. (2006). Mutational analysis of PRDM1 indicates a tumor-suppressor role in diffuse large B-cell lymphomas. Blood 107: 4090-4100 [Abstract] [Full Text]  
• Davis, C. A., Haberland, M., Arnold, M. A., Sutherland, L. B., McDonald, O. G., Richardson, J. A., Childs, G., Harris, S., Owens, G. K., Olson, E. N. (2006). PRISM/PRDM6, a Transcriptional Repressor That Promotes the Proliferative Gene Program in Smooth Muscle Cells.. Mol. Cell. Biol. 26: 2626-2636 [Abstract] [Full Text]  
• Pasqualucci, L., Compagno, M., Houldsworth, J., Monti, S., Grunn, A., Nandula, S. V., Aster, J. C., Murty, V. V., Shipp, M. A., Dalla-Favera, R. (2006). Inactivation of the PRDM1/BLIMP1 gene in diffuse large B cell lymphoma.. J. Exp. Med. 203: 311-317 [Abstract] [Full Text]  
• Taubenheim, N., von Hornung, M., Durandy, A., Warnatz, K., Corcoran, L., Peter, H.-H., Eibel, H. (2005). Defined Blocks in Terminal Plasma Cell Differentiation of Common Variable Immunodeficiency Patients. J. Immunol. 175: 5498-5503 [Abstract] [Full Text]  
• Kuo, T. C., Calame, K. L. (2004). B Lymphocyte-Induced Maturation Protein (Blimp)-1, IFN Regulatory Factor (IRF)-1, and IRF-2 Can Bind to the Same Regulatory Sites. J. Immunol. 173: 5556-5563 [Abstract] [Full Text]  
• Sciammas, R., Davis, M. M. (2004). Modular Nature of Blimp-1 in the Regulation of Gene Expression during B Cell Maturation. J. Immunol. 172: 5427-5440 [Abstract] [Full Text]  
• Vanhoutteghem, A., Djian, P. (2004). Basonuclin 2: An extremely conserved homolog of the zinc finger protein basonuclin. Proc. Natl. Acad. Sci. USA 101: 3468-3473 [Abstract] [Full Text]  
• Gyory, I., Fejer, G., Ghosh, N., Seto, E., Wright, K. L. (2003). Identification of a Functionally Impaired Positive Regulatory Domain I Binding Factor 1 Transcription Repressor in Myeloma Cell Lines. J. Immunol. 170: 3125-3133 [Abstract] [Full Text]  
• Lin, K.-I, Angelin-Duclos, C., Kuo, T. C., Calame, K. (2002). Blimp-1-Dependent Repression of Pax-5 Is Required for Differentiation of B Cells to Immunoglobulin M-Secreting Plasma Cells. Mol. Cell. Biol. 22: 4771-4780 [Abstract] [Full Text]  
• Tunyaplin, C., Shapiro, M. A., Calame, K. L. (2000). Characterization of the B lymphocyte-induced maturation protein-1 (Blimp-1) gene, mRNA isoforms and basal promoter. Nucleic Acids Res 28: 4846-4855 [Abstract] [Full Text]  
• Angelin-Duclos, C., Cattoretti, G., Lin, K.-I, Calame, K. (2000). Commitment of B Lymphocytes to a Plasma Cell Fate Is Associated with Blimp-1 Expression In Vivo. J. Immunol. 165: 5462-5471 [Abstract] [Full Text]  
• Avram, D., Fields, A., Top, K. P. O., Nevrivy, D. J., Ishmael, J. E., Leid, M. (2000). Isolation of a Novel Family of C2H2 Zinc Finger Proteins Implicated in Transcriptional Repression Mediated by Chicken Ovalbumin Upstream Promoter Transcription Factor (COUP-TF) Orphan Nuclear Receptors. J. Biol. Chem. 275: 10315-10322 [Abstract] [Full Text]  
• Iuchi, S., Green, H. (1999). Basonuclin, a zinc finger protein of keratinocytes and reproductive germ cells, binds to the rRNA gene promoter. Proc. Natl. Acad. Sci. USA 96: 9628-9632 [Abstract] [Full Text]  
• Messika, E. J., Lu, P. S., Sung, Y.-J., Yao, T., Chi, J.-T., Chien, Y.-h., Davis, M. M. (1998). Differential Effect of B Lymphocyte-induced Maturation Protein (Blimp-1) Expression on Cell Fate during B Cell Development. J. Exp. Med. 188: 515-525 [Abstract] [Full Text]  
• Braganca, J., Genin, P., Bandu, M.-T., Darracq, N., Vignal, M., Casse, C., Doly, J., Civas, A. (1997). Synergism between Multiple Virus-induced Factor-binding Elements Involved in the Differential Expression of Interferon A Genes. J. Biol. Chem. 272: 22154-22162 [Abstract] [Full Text]  
• Iuchi, S., Green, H. (1997). Nuclear localization of basonuclin in human keratinocytes and the role of phosphorylation. Proc. Natl. Acad. Sci. USA 94: 7948-7953 [Abstract] [Full Text]  
• Lipkin, S M, Naar, A M, Kalla, K A, Sack, R A, Rosenfeld, M G (1993). Identification of a novel zinc finger protein binding a conserved element critical for Pit-1-dependent growth hormone gene expression.. Genes Dev. 7: 1674-1687 [Abstract]  
• Funahashi, J, Sekido, R, Murai, K, Kamachi, Y, Kondoh, H (1993). Delta-crystallin enhancer binding protein delta EF1 is a zinc finger-homeodomain protein implicated in postgastrulation embryogenesis. Development 119: 433-446 [Abstract]