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Mol Cell Biol. 1992 May; 12(5): 2186-2192

The transport of proteins into the nucleus requires the 70-kilodalton heat shock protein or its cytosolic cognate.

Department of Biochemistry, New York University School of Medicine, New York 10016.

ABSTRACT

The 70-kDa heat shock protein hsp70 and its constitutively expressed cognate, hsc70, are abundant proteins implicated in a number of cellular processes. When a permeabilized cell system for examining the transport of proteins into the nucleus is depleted of hsc70 and hsp70, either by affinity chromatography on ATP-agarose or with antibodies against these proteins, nuclear transport activity is lost. Full activity is restored by the addition of HeLa proteins that bind to ATP-agarose. hsc70 and hsp70 are the active factors, since activity is also fully restored by the addition of either recombinant hsc70 or hsp70 which has been bacterially expressed and highly purified. The restoration of activity is saturable. The transport system requires other cytosolic factors as well, including at least one protein that is sensitive to inactivation by N-ethylmaleimide, but neither hsc70 nor hsp70 is the sensitive protein.

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• Kose, S., Furuta, M., Koike, M., Yoneda, Y., Imamoto, N. (2005). The 70-kD heat shock cognate protein (hsc70) facilitates the nuclear export of the import receptors. J. Cell Biol. 171: 19-25 [Abstract] [Full Text]  
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• Yu, L.-G., Andrews, N., Weldon, M., Gerasimenko, O. V., Campbell, B. J., Singh, R., Grierson, I., Petersen, O. H., Rhodes, J. M. (2002). An N-terminal Truncated Form of Orp150 Is a Cytoplasmic Ligand for the Anti-proliferative Mushroom Agaricus bisporus Lectin and Is Required for Nuclear Localization Sequence-dependent Nuclear Protein Import. J. Biol. Chem. 277: 24538-24545 [Abstract] [Full Text]  
• Cornford, P. A., Dodson, A. R., Parsons, K. F., Desmond, A. D., Woolfenden, A., Fordham, M., Neoptolemos, J. P., Ke, Y., Foster, C. S. (2000). Heat Shock Protein Expression Independently Predicts Clinical Outcome in Prostate Cancer. Cancer Res. 60: 7099-7105 [Abstract] [Full Text]  
• Bernstein, S. L., Liu, A. M.-H., Hansen, B. C., Somiari, R. I. (2000). Heat Shock Cognate-70 Gene Expression Declines during Normal Aging of the Primate Retina. IOVS 41: 2857-2862 [Abstract] [Full Text]  
• Nylandsted, J., Rohde, M., Brand, K., Bastholm, L., Elling, F., Jaattela, M. (2000). Selective depletion of heat shock protein 70 (Hsp70) activates a tumor-specific death program that is independent of caspases and bypasses Bcl-2. Proc. Natl. Acad. Sci. USA 97: 7871-7876 [Abstract] [Full Text]  
• Saphire, A. C. S., Guan, T., Schirmer, E. C., Nemerow, G. R., Gerace, L. (2000). Nuclear Import of Adenovirus DNA in Vitro Involves the Nuclear Protein Import Pathway and hsc70. J. Biol. Chem. 275: 4298-4304 [Abstract] [Full Text]  
• Srivastava, M., Pollard, H. B. (1999). Molecular dissection of nucleolin's role in growth and cell proliferation: new insights. FASEB J. 13: 1911-1922 [Abstract] [Full Text]  
• Shulga, N., James, P., Craig, E. A., Goldfarb, D. S. (1999). A Nuclear Export Signal Prevents Saccharomyces cerevisiae Hsp70 Ssb1p from Stimulating Nuclear Localization Signal-directed Nuclear Transport. J. Biol. Chem. 274: 16501-16507 [Abstract] [Full Text]  
• Bray, P. J., Du, B., Mejia, V. M., Hao, S. C., Deutsch, E., Fu, C., Wilson, R. C., Hanauske-Abel, H., McCaffrey, T. A. (1999). Glucocorticoid Resistance Caused by Reduced Expression of the Glucocorticoid Receptor in Cells From Human Vascular Lesions. Arterioscler. Thromb. Vasc. Bio. 19: 1180-1189 [Abstract] [Full Text]  
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• Demand, J., Lüders, J., Höhfeld, J. (1998). The Carboxy-Terminal Domain of Hsc70 Provides Binding Sites for a Distinct Set of Chaperone Cofactors. Mol. Cell. Biol. 18: 2023-2028 [Abstract] [Full Text]  
• Lu, D., Yang, H., Raizada, M. K. (1998). Involvement of p62 Nucleoporin in Angiotensin II-Induced Nuclear Translocation of STAT3 in Brain Neurons. J. Neurosci. 18: 1329-1336 [Abstract] [Full Text]  
• Pratt, W. B., Toft, D. O. (1997). Steroid Receptor Interactions with Heat Shock Protein and Immunophilin Chaperones. Endocr. Rev. 18: 306-360 [Abstract] [Full Text]  
• Agarraberes, F. A., Terlecky, S. R., Dice, J. F. (1997). An Intralysosomal hsp70 Is Required for a Selective Pathway of Lysosomal Protein Degradation. J. Cell Biol. 137: 825-834 [Abstract] [Full Text]  
• Kelley, W. L., Georgopoulos, C. (1997). The T/t common exon of simian virus 40, JC, and BK polyomavirus T antigens can functionally replace the J-domain of the Escherichia coli DnaJ molecular chaperone. Proc. Natl. Acad. Sci. USA 94: 3679-3684 [Abstract] [Full Text]  
• Nadler, S. G., Tritschler, D., Haffar, O. K., Blake, J., Bruce, A. G., Cleaveland, J. S. (1997). Differential Expression and Sequence-specific Interaction of Karyopherin alpha with Nuclear Localization Sequences. J. Biol. Chem. 272: 4310-4315 [Abstract] [Full Text]  
• Leung, S.-M., Hightower, L. E. (1997). A 16-kDa Protein Functions as a New Regulatory Protein for Hsc70 Molecular Chaperone and Is Identified as a Member of the Nm23/Nucleoside Diphosphate Kinase Family. J. Biol. Chem. 272: 2607-2614 [Abstract] [Full Text]  
• Kourtz, L., Ko, K. (1997). The Early Stage of Chloroplast Protein Import Involves Com70. J. Biol. Chem. 272: 2808-2813 [Abstract] [Full Text]  
• Liu, Q., Levy, E. J., Chirico, W. J. (1996). N-Ethylmaleimide Inactivates a Nucleotide-free Hsp70 Molecular Chaperone. J. Biol. Chem. 271: 29937-29944 [Abstract] [Full Text]  
• Buxbaum, E., Woodman, P. G. (1996). The speed of partial reactions of the uncoating ATPase Hsc70 depends on the source of coated vesicles. J. Cell Sci. 109: 705-711 [Abstract]  
• Saitoh, H., Dasso, M. (1995). The RCC1 Protein Interacts with Ran, RanBP1, hsc70, and a 340-kDa Protein in Xenopus Extracts. J. Biol. Chem. 270: 10658-10663 [Abstract] [Full Text]  
• Imamoto, N., Tachibana, T., Matsubae, M., Yoneda, Y. (1995). A Karyophilic Protein Forms a Stable Complex with Cytoplasmic Components Prior to Nuclear Pore Binding. J. Biol. Chem. 270: 8559-8565 [Abstract] [Full Text]  
• Pokrywka, N. J., Goldfarb, D. S. (1995). Nuclear Export Pathways of tRNA and 40 S Ribosomes Include both Common and Specific Intermediates. J. Biol. Chem. 270: 3619-3624 [Abstract] [Full Text]  
• Liao, J., Lowthert, L. A., Ghori, N., Omary, M. B. (1995). The 70-kDa Heat Shock Proteins Associate with Glandular Intermediate Filaments in an ATP-dependent Manner. J. Biol. Chem. 270: 915-922 [Abstract] [Full Text]  
• Cserpan, I, Udvardy, A (1995). The mechanism of nuclear transport of natural or artificial transport substrates in digitonin-permeabilized cells. J. Cell Sci. 108: 1849-1861 [Abstract]  
• Buxbaum, E, Woodman, P. (1995). Selective action of uncoating ATPase towards clathrin-coated vesicles from brain. J. Cell Sci. 108: 1295-1306 [Abstract]  
• Vancurova, I, Paine, T., Lou, W, Paine, P. (1995). Nucleoplasmin associates with and is phosphorylated by casein kinase II. J. Cell Sci. 108: 779-787 [Abstract]  
• Wang, J, Satoh, M, Pierani, A, Schmitt, J, Chou, C., Stunnenberg, H., Roeder, R., Reeves, W. (1994). Assembly and DNA binding of recombinant Ku (p70/p80) autoantigen defined by a novel monoclonal antibody specific for p70/p80 heterodimers. J. Cell Sci. 107: 3223-3233 [Abstract]  
• Honing, S, Kreimer, G, Robenek, H, Jockusch, B. (1994). Receptor-mediated endocytosis is sensitive to antibodies against the uncoating ATPase (hsc70). J. Cell Sci. 107: 1185-1196 [Abstract]  
• Dauvois, S, White, R, Parker, M. (1993). The antiestrogen ICI 182780 disrupts estrogen receptor nucleocytoplasmic shuttling. J. Cell Sci. 106: 1377-1388 [Abstract]  
• Dingwall, C, Laskey, R (1992). The nuclear membrane. Science 258: 942-947 [Abstract]