This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wainwright, S M
Right arrow Articles by Ish-Horowicz, D
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wainwright, S M
Right arrow Articles by Ish-Horowicz, D

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 June; 12(6): 2475-2483

Point mutations in the Drosophila hairy gene demonstrate in vivo requirements for basic, helix-loop-helix, and WRPW domains.

S M Wainwright and D Ish-Horowicz

Department of Zoology, University of Oxford, England.

ABSTRACT

The Drosophila pair-rule gene, hairy (h), encodes a nuclear basic helix-loop-helix (bHLH) protein that regulates embryonic segmentation and adult bristle patterning. In both cases, the h protein behaves as a transcriptional repressor. In this study, we determined the molecular nature of 12 h alleles. One mutation maps within the HLH domain, consistent with h function requiring homodimerization or heterodimerization with other HLH proteins. A second mutation lies in the basic domain, suggesting that DNA binding is required for h activity. Several mutations show that the h C terminus, in particular the WRPW domain, is also required for h activity, perhaps by interacting with other proteins to mediate transcriptional repression. We show that the h protein in Drosophila virilis closely resembles that in D. melanogaster and includes completely conserved bHLH and WRPW domains.

Mol Cell Biol. 1992 June; 12(6): 2475-2483




This article has been cited by other articles:

  • Fischer, A., Gessler, M. (2007). Delta Notch and then? Protein interactions and proposed modes of repression by Hes and Hey bHLH factors. Nucleic Acids Res 35: 4583-4596 [Abstract] [Full Text]  
  • Ninkovic, J., Tallafuss, A., Leucht, C., Topczewski, J., Tannhauser, B., Solnica-Krezel, L., Bally-Cuif, L. (2005). Inhibition of neurogenesis at the zebrafish midbrain-hindbrain boundary by the combined and dose-dependent activity of a new hairy/E(spl) gene pair. Development 132: 75-88 [Abstract] [Full Text]  
  • Hans, S., Scheer, N., Riedl, I., Weizsacker, E. v., Blader, P., Campos-Ortega, J. A. (2004). her3, a zebrafish member of the hairy-E(spl) family, is repressed by Notch signalling. Development 131: 2957-2969 [Abstract] [Full Text]  
  • Secombe, J., Parkhurst, S. M. (2004). Drosophila Topors Is a RING Finger-containing Protein That Functions as a Ubiquitin-protein Isopeptide Ligase for the Hairy Basic Helix-Loop-Helix Repressor Protein. J. Biol. Chem. 279: 17126-17133 [Abstract] [Full Text]  
  • Brown, S. J., Fellers, J. P., Shippy, T. D., Richardson, E. A., Maxwell, M., Stuart, J. J., Denell, R. E. (2002). Sequence of the Tribolium castaneum Homeotic Complex: The Region Corresponding to the Drosophila melanogaster Antennapedia Complex. Genetics 160: 1067-1074 [Abstract] [Full Text]  
  • Iso, T., Sartorelli, V., Poizat, C., Iezzi, S., Wu, H.-Y., Chung, G., Kedes, L., Hamamori, Y. (2001). HERP, a Novel Heterodimer Partner of HES/E(spl) in Notch Signaling. Mol. Cell. Biol. 21: 6080-6089 [Abstract] [Full Text]  
  • Alper, S, Kenyon, C (2001). REF-1, a protein with two bHLH domains, alters the pattern of cell fusion in C. elegans by regulating Hox protein activity. Development 128: 1793-1804 [Abstract]  
  • Castella, P., Sawai, S., Nakao, K., Wagner, J. A., Caudy, M. (2000). HES-1 Repression of Differentiation and Proliferation in PC12 Cells: Role for the Helix 3-Helix 4 Domain in Transcription Repression. Mol. Cell. Biol. 20: 6170-6183 [Abstract] [Full Text]  
  • Kim, J., Kerr, J. Q., Min, G.-S. (2000). Molecular heterochrony in the early development of Drosophila. Proc. Natl. Acad. Sci. USA 97: 212-216 [Abstract] [Full Text]  
  • Jimenez, G., Verrijzer, C. P., Ish-Horowicz, D. (1999). A Conserved Motif in Goosecoid Mediates Groucho-Dependent Repression in Drosophila Embryos. Mol. Cell. Biol. 19: 2080-2087 [Abstract] [Full Text]  
  • Zhang, H., Levine, M. (1999). Groucho and dCtBP mediate separate pathways of transcriptional repression in the Drosophila embryo. Proc. Natl. Acad. Sci. USA 96: 535-540 [Abstract] [Full Text]  
  • Goldstein, R., Jimenez, G, Cook, O, Gur, D, Paroush, Z (1999). Huckebein repressor activity in Drosophila terminal patterning is mediated by Groucho. Development 126: 3747-3755 [Abstract]  
  • Takke, C, Dornseifer, P, v Weizsacker, E, Campos-Ortega, J. (1999). her4, a zebrafish homologue of the Drosophila neurogenic gene E(spl), is a target of NOTCH signalling. Development 126: 1811-1821 [Abstract]  
  • Kim, H. K., Siu, G. (1998). The Notch Pathway Intermediate HES-1 Silences CD4 Gene Expression. Mol. Cell. Biol. 18: 7166-7175 [Abstract] [Full Text]  
  • Levanon, D., Goldstein, R. E., Bernstein, Y., Tang, H., Goldenberg, D., Stifani, S., Paroush, Z.'e., Groner, Y. (1998). Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors. Proc. Natl. Acad. Sci. USA 95: 11590-11595 [Abstract] [Full Text]  
  • Alifragis, P., Poortinga, G., Parkhurst, S. M., Delidakis, C. (1997). A network of interacting transcriptional regulators involved in Drosophila neural fate specification revealed by the yeast two-hybrid system. Proc. Natl. Acad. Sci. USA 94: 13099-13104 [Abstract] [Full Text]  
  • Giebel, B., Campos-Ortega, J. A. (1997). Functional dissection of the Drosophila Enhancer of split protein, a suppressor of neurogenesis. Proc. Natl. Acad. Sci. USA 94: 6250-6254 [Abstract] [Full Text]  
  • Paroush, Z, Wainwright, S., Ish-Horowicz, D (1997). Torso signalling regulates terminal patterning in Drosophila by antagonising Groucho-mediated repression. Development 124: 3827-3834 [Abstract]  
  • Wrischnik, L., Kenyon, C. (1997). The role of lin-22, a hairy/enhancer of split homolog, in patterning the peripheral nervous system of C. elegans. Development 124: 2875-2888 [Abstract]  
  • Wettstein, D., Turner, D., Kintner, C (1997). The Xenopus homolog of Drosophila Suppressor of Hairless mediates Notch signaling during primary neurogenesis. Development 124: 693-702 [Abstract]  
  • Giangrande, A (1995). Proneural genes influence gliogenesis in Drosophila. Development 121: 429-438 [Abstract]  
  • Tsai, C, Gergen, P (1995). Pair-rule expression of the Drosophila fushi tarazu gene: a nuclear receptor response element mediates the opposing regulatory effects of runt and hairy. Development 121: 453-462 [Abstract]  
  • Van Doren, M, Bailey, A M, Esnayra, J, Ede, K, Posakony, J W (1994). Negative regulation of proneural gene activity: hairy is a direct transcriptional repressor of achaete.. Genes Dev. 8: 2729-2742 [Abstract]  
  • Ohsako, S, Hyer, J, Panganiban, G, Oliver, I, Caudy, M (1994). Hairy function as a DNA-binding helix-loop-helix repressor of Drosophila sensory organ formation.. Genes Dev. 8: 2743-2755 [Abstract]  
  • Orenic, T., Held, L., Paddock, S., Carroll, S. (1993). The spatial organization of epidermal structures: hairy establishes the geometrical pattern of Drosophila leg bristles by delimiting the domains of achaete expression. Development 118: 9-20 [Abstract]  
  • Lardelli, M, Ish-Horowicz, D (1993). Drosophila hairy pair-rule gene regulates embryonic patterning outside its apparent stripe domains. Development 118: 255-266 [Abstract]  
  • Langeland, J., Carroll, S. (1993). Conservation of regulatory elements controlling hairy pair-rule stripe formation. Development 117: 585-596 [Abstract]  
  • Parkhurst, S., Lipshitz, H., Ish-Horowicz, D (1993). achaete-scute feminizing activities and Drosophila sex determination. Development 117: 737-749 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»