This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by You, Y
Right arrow Articles by Shyu, A B
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by You, Y
Right arrow Articles by Shyu, A B

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 July; 12(7): 2931-2940

U-rich sequence-binding proteins (URBPs) interacting with a 20-nucleotide U-rich sequence in the 3' untranslated region of c-fos mRNA may be involved in the first step of c-fos mRNA degradation.

Y You, C Y Chen and A B Shyu

Department of Biochemistry and Molecular Biology, University of Texas, Health Science Center, Houston Medical School 77030.

ABSTRACT

Rapid decay of the c-fos transcript plays a critical role in controlling transforming potential of the c-fos proto-oncogene. One of the mRNA instability determinants is a 75-nucleotide AU-rich element (ARE) present in the 3' untranslated region of the c-fos transcript. It appears to control two steps in the process of c-fos mRNA degradation: removal of the poly(A) tail, which does not require the AUUUA motifs, and subsequent degradation of deadenylated mRNA, which appears to be dependent on the AUUUA motifs. In this study, we report the identification of four U-rich sequence binding proteins (URBPs) that specifically interact with a 20-nucleotide U-rich sequence within the c-fos ARE. Gel mobility shift assay and competition experiments showed that these protein factors form three specific band-shifted complexes with the c-fos ARE. Binding activity of one of the protein factors, a 37-kDa protein, is significantly affected by serum induction and by pretreatment of cells with drugs known to stabilize many of the immediate-early gene mRNAs. Combining UV cross-linking with a new approach, designated sequential RNase digestion, we were able to better determine the molecular masses of these cellular proteins. The binding sites for the four proteins were all mapped to a 20-nucleotide U-rich sequence located at the 3' half of the c-fos ARE, which contains no AUUUA pentanucleotides but stretches of uridylate residues. Single U-to-A point mutations in each of the three AUUUA motifs within the c-fos ARE have little effect on formation of the mobility-shifted complexes. Our data indicate c-fos ARE-protein interaction involves recognition of U stretches rather than recognition of the AUUUA motifs. We propose that UTBP binding may be involved in the first step, removal of the Poly(A) tail, in the c-fos ARE-mediated decay pathway.

Mol Cell Biol. 1992 July; 12(7): 2931-2940




This article has been cited by other articles:

  • Zou, T., Rao, J. N., Liu, L., Marasa, B. S., Keledjian, K. M., Zhang, A.-H., Xiao, L., Bass, B. L., Wang, J.-Y. (2005). Polyamine depletion induces nucleophosmin modulating stability and transcriptional activity of p53 in intestinal epithelial cells. Am. J. Physiol. Cell Physiol. 289: C686-C696 [Abstract] [Full Text]  
  • Hajarnis, S., Schroeder, J. M., Curthoys, N. P. (2005). 3'-Untranslated Region of Phosphoenolpyruvate Carboxykinase mRNA Contains Multiple Instability Elements That Bind AUF1. J. Biol. Chem. 280: 28272-28280 [Abstract] [Full Text]  
  • Puga, I., Lainez, B., Fernandez-Real, J. M., Buxade, M., Broch, M., Vendrell, J., Espel, E. (2005). A Polymorphism in the 3' Untranslated Region of the Gene for Tumor Necrosis Factor Receptor 2 Modulates Reporter Gene Expression. Endocrinology 146: 2210-2220 [Abstract] [Full Text]  
  • CHEN, C.-Y. A., XU, N., ZHU, W., SHYU, A.-B. (2004). Functional dissection of hnRNP D suggests that nuclear import is required before hnRNP D can modulate mRNA turnover in the cytoplasm. RNA 10: 669-680 [Abstract] [Full Text]  
  • Shao, R., Shi, Z., Gotwals, P. J., Koteliansky, V. E., George, J., Rockey, D. C. (2003). Cell and Molecular Regulation of Endothelin-1 Production during Hepatic Wound Healing. Mol. Biol. Cell 14: 2327-2341 [Abstract] [Full Text]  
  • Harizi, H., Juzan, M., Moreau, J.-F., Gualde, N. (2003). Prostaglandins Inhibit 5-Lipoxygenase-Activating Protein Expression and Leukotriene B4 Production from Dendritic Cells Via an IL-10-Dependent Mechanism. J. Immunol. 170: 139-146 [Abstract] [Full Text]  
  • Duquette, M., Laneuville, O. (2002). Translational Regulation of Prostaglandin Endoperoxide H Synthase-1 mRNA in Megakaryocytic MEG-01 Cells. SPECIFIC PROTEIN BINDING TO A CONSERVED 20-NUCLEOTIDE CIS ELEMENT IN THE 3'-UNTRANSLATED REGION. J. Biol. Chem. 277: 44631-44637 [Abstract] [Full Text]  
  • Lapucci, A., Donnini, M., Papucci, L., Witort, E., Tempestini, A., Bevilacqua, A., Nicolin, A., Brewer, G., Schiavone, N., Capaccioli, S. (2002). AUF1 Is a bcl-2 A + U-rich Element-binding Protein Involved in bcl-2 mRNA Destabilization during Apoptosis. J. Biol. Chem. 277: 16139-16146 [Abstract] [Full Text]  
  • Xu, N., Chen, C.-Y. A., Shyu, A.-B. (2001). Versatile Role for hnRNP D Isoforms in the Differential Regulation of Cytoplasmic mRNA Turnover. Mol. Cell. Biol. 21: 6960-6971 [Abstract] [Full Text]  
  • Li, L., Rao, J. N., Guo, X., Liu, L., Santora, R., Bass, B. L., Wang, J.-Y. (2001). Polyamine depletion stabilizes p53 resulting in inhibition of normal intestinal epithelial cell proliferation. Am. J. Physiol. Cell Physiol. 281: C941-C953 [Abstract] [Full Text]  
  • Balmer, L. A., Beveridge, D. J., Jazayeri, J. A., Thomson, A. M., Walker, C. E., Leedman, P. J. (2001). Identification of a Novel AU-Rich Element in the 3' Untranslated Region of Epidermal Growth Factor Receptor mRNA That Is the Target for Regulated RNA-Binding Proteins. Mol. Cell. Biol. 21: 2070-2084 [Abstract] [Full Text]  
  • Farhana, L., Boyanapalli, M., Tschang, S.-H. R., Sun, R.-J., Hsu, C. K. A., Zhang, Y.-X., Fontana, J. A., Rishi, A. K. (2000). Okadaic Acid-mediated Induction of the c-fos Gene in Estrogen Receptor-negative Human Breast Carcinoma Cells Utilized, in Part, Posttranscriptional Mechanisms Involving Adenosine-Uridine-rich Elements. Cell Growth Differ. 11: 541-550 [Abstract] [Full Text]  
  • Gale, M. Jr., Tan, S.-L., Katze, M. G. (2000). Translational Control of Viral Gene Expression in Eukaryotes. Microbiol. Mol. Biol. Rev. 64: 239-280 [Abstract] [Full Text]  
  • Brooks, S. A., Rigby, W. F. C. (2000). Characterization of the mRNA ligands bound by the RNA binding protein hnRNP A2 utilizing a novel in vivo technique. Nucleic Acids Res 28: e49-e49 [Abstract] [Full Text]  
  • Loflin, P., Chen, C.-Y. A., Shyu, A.-B. (1999). Unraveling a cytoplasmic role for hnRNP D in the in vivo mRNA destabilization directed by the AU-rich element. Genes Dev. 13: 1884-1897 [Abstract] [Full Text]  
  • Erondu, N. E., Nwankwo, J., Zhong, Y., Boes, M., Dake, B., Bar, R. S. (1999). Transcriptional and Posttranscriptional Regulation of Insulin-Like Growth Factor Binding Protein-3 by Cyclic Adenosine 3',5'-Monophosphate: Messenger RNA Stabilization Is Accompanied by Decreased Binding of a 42-kDa Protein to a Uridine-Rich Domain in the 3'-Untranslated Region. Mol. Endocrinol. 13: 495-504 [Abstract] [Full Text]  
  • Spicher, A., Guicherit, O. M., Duret, L., Aslanian, A., Sanjines, E. M., Denko, N. C., Giaccia, A. J., Blau, H. M. (1998). Highly Conserved RNA Sequences That Are Sensors of Environmental Stress. Mol. Cell. Biol. 18: 7371-7382 [Abstract] [Full Text]  
  • Fraboulet, S., Boudouresque, F., Delfino, C., Ouafik, L'H. (1998). Identification of a Novel cis-Element in the 3'-Untranslated Region of Mammalian Peptidylglycine {alpha}-Amidating Monooxygenase Messenger Ribonucleic Acid. Endocrinology 139: 894-904 [Abstract] [Full Text]  
  • Claffey, K. P., Shih, S.-C., Mullen, A., Dziennis, S., Cusick, J. L., Abrams, K. R., Lee, S. W., Detmar, M. (1998). Identification of a Human VPF/VEGF 3' Untranslated Region Mediating Hypoxia-induced mRNA Stability. Mol. Biol. Cell 9: 469-481 [Abstract] [Full Text]  
  • Nuglozeh, E., Mbikay, M., Stewart, D. J., Legault, L. (1997). Gene expression of natriuretic peptide receptors in rats with DOCA-salt hypertension. Am. J. Physiol. Cell Physiol. 273: C1427-C1434 [Abstract] [Full Text]  
  • Laterza, O. F., Hansen, W. R., Taylor, L., Curthoys, N. P. (1997). Identification of an mRNA-binding Protein and the Specific Elements That May Mediate the pH-responsive Induction of Renal Glutaminase mRNA. J. Biol. Chem. 272: 22481-22488 [Abstract] [Full Text]  
  • Chagnovich, D., Fayos, B. E., Cohn, S. L. (1996). Differential Activity of ELAV-like RNA-binding Proteins in Human Neuroblastoma. J. Biol. Chem. 271: 33587-33591 [Abstract] [Full Text]  
  • Peng, H., Lever, J. E. (1995). Regulation of Na[IMAGE]-coupled Glucose Transport in LLC-PK(1) Cells. J. Biol. Chem. 270: 23996-24003 [Abstract] [Full Text]  
  • Caponigro, G, Parker, R (1995). Multiple functions for the poly(A)-binding protein in mRNA decapping and deadenylation in yeast.. Genes Dev. 9: 2421-2432 [Abstract]  
  • Sadot, E, Barg, J, Rasouly, D, Lazarovici, P, Ginzburg, I (1995). Short- and long-term mechanisms of tau regulation in PC12 cells. J. Cell Sci. 108: 2857-2864 [Abstract]  
  • Brown, B D, Zipkin, I D, Harland, R M (1993). Sequence-specific endonucleolytic cleavage and protection of mRNA in Xenopus and Drosophila.. Genes Dev. 7: 1620-1631 [Abstract]  
  • Lowell, J E, Rudner, D Z, Sachs, A B (1992). 3'-UTR-dependent deadenylation by the yeast poly(A) nuclease.. Genes Dev. 6: 2088-2099 [Abstract]  
  • Lee, W. Y., Loflin, P., Clancey, C. J., Peng, H., Lever, J. E. (2000). Cyclic Nucleotide Regulation of Na+/Glucose Cotransporter (SGLT1) mRNA Stability. INTERACTION OF A NUCLEOCYTOPLASMIC PROTEIN WITH A REGULATORY DOMAIN IN THE 3'-UNTRANSLATED REGION CRITICAL FOR STABILIZATION. J. Biol. Chem. 275: 33998-34008 [Abstract] [Full Text]  
  • Massillon, D. (2001). Regulation of the Glucose-6-phosphatase Gene by Glucose Occurs by Transcriptional and Post-transcriptional Mechanisms. DIFFERENTIAL EFFECT OF GLUCOSE AND XYLITOL. J. Biol. Chem. 276: 4055-4062 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»