This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maguire, J E Articles by Singer, D S Search for Related Content PubMed PubMed Citation Articles by Maguire, J E Articles by Singer, D S

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 July; 12(7): 3078-3086

In vivo function of regulatory DNA sequence elements of a major histocompatibility complex class I gene.

Experimental Immunology Branch, National Cancer Institute, Bethesda, Maryland 20892.

ABSTRACT

Major histocompatibility complex class I genes are expressed in nearly all somatic tissues, although their level of expression varies. By analysis of a set of promoter deletion mutants introduced into transgenic mice, a complex regulatory element, consisting of overlapping enhancer and silencer activities, is demonstrated to function as a tissue-specific regulator of class I expression. The enhancer activity predominates in lymphoid tissues but not in nonlymphoid tissues. In contrast to the tissue-specific functions of the complex regulatory element, a second novel silencer element is shown to function in both lymphoid and nonlymphoid tissues. The complement of DNA-binding factors in different cell lines is shown to correlate with the levels of class I expression.

This article has been cited by other articles:

• Giuliani, C, Saji, M, Bucci, I, Fiore, G, Liberatore, M, Singer, D S, Monaco, F, Kohn, L D, Napolitano, G (2006). Transcriptional regulation of major histocompatibility complex class I gene by insulin and IGF-I in FRTL-5 thyroid cells.. J Endocrinol 189: 605-615 [Abstract] [Full Text]  
• Howcroft, T. K., Raval, A., Weissman, J. D., Gegonne, A., Singer, D. S. (2003). Distinct Transcriptional Pathways Regulate Basal and Activated Major Histocompatibility Complex Class I Expression. Mol. Cell. Biol. 23: 3377-3391 [Abstract] [Full Text]  
• Weissman, J. D., Howcroft, T. K., Singer, D. S. (2000). TAFII250-independent Transcription Can Be Conferred on a TAFII250-dependent Basal Promoter by Upstream Activators. J. Biol. Chem. 275: 10160-10167 [Abstract] [Full Text]  
• Howcroft, T. K., Murphy, C., Weissman, J. D., Huber, S. J., Sawadogo, M., Singer, D. S. (1999). Upstream Stimulatory Factor Regulates Major Histocompatibility Complex Class I Gene Expression: the U2Delta E4 Splice Variant Abrogates E-Box Activity. Mol. Cell. Biol. 19: 4788-4797 [Abstract] [Full Text]  
• Saji, M., Shong, M., Napolitano, G., Palmer, L. A., Taniguchi, S.-I., Ohmori, M., Ohta, M., Suzuki, K., Kirshner, S. L., Giuliani, C., Singer, D. S., Kohn, L. D. (1997). Regulation of Major Histocompatibility Complex Class I Gene Expression in Thyroid Cells. ROLE OF THE cAMP RESPONSE ELEMENT-LIKE SEQUENCE. J. Biol. Chem. 272: 20096-20107 [Abstract] [Full Text]  
• Snyder, S. R., Wang, J., Waring, J. F., Ginder, G. D. (2001). Identification of CCAAT Displacement Protein (CDP/cut) as a Locus-specific Repressor of Major Histocompatibility Complex Gene Expression in Human Tumor Cells. J. Biol. Chem. 276: 5323-5330 [Abstract] [Full Text]