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Mol Cell Biol. 1992 July; 12(7): 3224-3234

Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins.

Section de Biologie, Instiut Curie, Centre Universitaire, Orsay, France.

ABSTRACT

Eleven suppressors of the radiation sensitivity of Saccharomyces cerevisiae diploids lacking the Srs2 helicase were analyzed and found to contain codominant mutations in the RAD51 gene known to be involved in recombinational repair and in genetic recombination. These mutant alleles confer an almost complete block in recombinational repair, as does deletion of RAD51, but heterozygous mutant alleles suppress the defects of srs2::LEU2 cells and are semidominant in Srs2+ cells. The results of this study are interpreted to mean that wild-type Rad51 protein binds to single-stranded DNA and that the semidominant mutations do not prevent this binding. The cloning and sequencing of RAD51 indicated that the gene encodes a predicted 400-amino-acid protein with a molecular mass of 43 kDa. Sequence comparisons revealed homologies to domains of Escherichia coli RecA protein predicted to be involved in DNA binding, ATP binding, and ATP hydrolysis. The expression of RAD51, measured with a RAD51-lacZ gene fusion, was found to be UV- and gamma-ray-inducible, with dose-dependent responses.

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• Arai, N., Ito, D., Inoue, T., Shibata, T., Takahashi, H. (2005). Heteroduplex Joint Formation by a Stoichiometric Complex of Rad51 and Rad52 of Saccharomyces cerevisiae. J. Biol. Chem. 280: 32218-32229 [Abstract] [Full Text]  
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• Takata, M., Sasaki, M. S., Sonoda, E., Fukushima, T., Morrison, C., Albala, J. S., Swagemakers, S. M. A., Kanaar, R., Thompson, L. H., Takeda, S. (2000). The Rad51 Paralog Rad51B Promotes Homologous Recombinational Repair. Mol. Cell. Biol. 20: 6476-6482 [Abstract] [Full Text]  
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• Mazin, A. V., Kowalczykowski, S. C. (1999). A novel property of the RecA nucleoprotein filament: activation of double- stranded DNA for strand exchange in trans. Genes Dev. 13: 2005-2016 [Abstract] [Full Text]  
• Khasanov, F. K., Savchenko, G. V., Bashkirova, E. V., Korolev, V. G., Heyer, W.-D., Bashkirov, V. I. (1999). A New Recombinational DNA Repair Gene From Schizosaccharomyces pombe With Homology to Escherichia coli RecA. Genetics 152: 1557-1572 [Abstract] [Full Text]  
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• Paques, F., Haber, J. E. (1999). Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 63: 349-404 [Abstract] [Full Text]  
• Huang, Y., Nakada, S., Ishiko, T., Utsugisawa, T., Datta, R., Kharbanda, S., Yoshida, K., Talanian, R. V., Weichselbaum, R., Kufe, D., Yuan, Z.-M. (1999). Role for Caspase-Mediated Cleavage of Rad51 in Induction of Apoptosis by DNA Damage. Mol. Cell. Biol. 19: 2986-2997 [Abstract] [Full Text]  
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• Paulovich, A. G., Armour, C. D., Hartwell, L. H. (1998). The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 Genes Are Required for Tolerating Irreparable, Ultraviolet-Induced DNA Damage. Genetics 150: 75-93 [Abstract] [Full Text]  
• Bishop, D. K., Ear, U., Bhattacharyya, A., Calderone, C., Beckett, M., Weichselbaum, R. R., Shinohara, A. (1998). Xrcc3 Is Required for Assembly of Rad51 Complexes in Vivo. J. Biol. Chem. 273: 21482-21488 [Abstract] [Full Text]  
• Namsaraev, E. A., Berg, P. (1998). Binding of Rad51p to DNA. INTERACTION OF Rad51p WITH SINGLE- AND DOUBLE-STRANDED DNA. J. Biol. Chem. 273: 6177-6182 [Abstract] [Full Text]  
• Derr, L. K. (1998). The Involvement of Cellular Recombination and Repair Genes in RNA-Mediated Recombination in Saccharomyces cerevisiae. Genetics 148: 937-945 [Abstract] [Full Text]  
• Tracy, R. B., Baumohl, J. K., Kowalczykowski, S. C. (1997). The preference for GT-rich DNA by the yeast Rad51 protein defines a set of universal pairing sequences. Genes Dev. 11: 3423-3431 [Abstract] [Full Text]  
• Li, Z., Golub, E. I., Gupta, R., Radding, C. M. (1997). Recombination activities of HsDmc1 protein, the meiotic human homolog of RecA protein. Proc. Natl. Acad. Sci. USA 94: 11221-11226 [Abstract] [Full Text]  
• Purandare, S. M., Patel, P. I. (1997). Recombination Hot Spots and Human Disease. Genome Res 7: 773-786 [Full Text]  
• Rice, M. C., Smith, S. T., Bullrich, F., Havre, P., Kmiec, E. B. (1997). Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis. Proc. Natl. Acad. Sci. USA 94: 7417-7422 [Abstract] [Full Text]  
• Sugiyama, T., Zaitseva, E. M., Kowalczykowski, S. C. (1997). A Single-stranded DNA-binding Protein Is Needed for Efficient Presynaptic Complex Formation by the Saccharomyces cerevisiae Rad51 Protein. J. Biol. Chem. 272: 7940-7945 [Abstract] [Full Text]  
• Gupta, R. C., Bazemore, L. R., Golub, E. I., Radding, C. M. (1997). Activities of human recombination protein Rad51. Proc. Natl. Acad. Sci. USA 94: 463-468 [Abstract] [Full Text]  
• Bai, Y, Symington, L S (1996). A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae.. Genes Dev. 10: 2025-2037 [Abstract]  
• Shen, Z., Cloud, K. G., Chen, D. J., Park, M. S. (1996). Specific Interactions between the Human RAD51 and RAD52 Proteins. J. Biol. Chem. 271: 148-152 [Abstract] [Full Text]  
• Donovan, J W, Milne, G T, Weaver, D T (1994). Homotypic and heterotypic protein associations control Rad51 function in double-strand break repair.. Genes Dev. 8: 2552-2562 [Abstract]  
• Sung, P (1994). Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein. Science 265: 1241-1243 [Abstract]  
• Milne, G T, Weaver, D T (1993). Dominant negative alleles of RAD52 reveal a DNA repair/recombination complex including Rad51 and Rad52.. Genes Dev. 7: 1755-1765 [Abstract]  
• Braybrooke, J. P., Spink, K. G., Thacker, J., Hickson, I. D. (2000). The RAD51 Family Member, RAD51L3, Is a DNA-stimulated ATPase That Forms a Complex with XRCC2. J. Biol. Chem. 275: 29100-29106 [Abstract] [Full Text]  
• Song, B., Sung, P. (2000). Functional Interactions among Yeast Rad51 Recombinase, Rad52 Mediator, and Replication Protein A in DNA Strand Exchange. J. Biol. Chem. 275: 15895-15904 [Abstract] [Full Text]