This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sistonen, L
Right arrow Articles by Morimoto, R I
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sistonen, L
Right arrow Articles by Morimoto, R I

 Previous Article  |  Next Article 

Mol Cell Biol. 1992 September; 12(9): 4104-4111

Activation of heat shock factor 2 during hemin-induced differentiation of human erythroleukemia cells.

L Sistonen, K D Sarge, B Phillips, K Abravaya and R I Morimoto

Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.

ABSTRACT

Hemin induces nonterminal differentiation of human K562 erythroleukemia cells, which is accompanied by the expression of certain erythroid cell-specific genes, such as the embryonic and fetal globins, and elevated expression of the stress genes hsp70, hsp90, and grp78/BiP. Previous studies revealed that, as during heat shock, transcriptional induction of hsp70 in hemin-treated cells is mediated by activation of heat shock transcription factor (HSF), which binds to the heat shock element (HSE). We report here that hemin activates the DNA-binding activity of HSF2, whereas heat shock induces predominantly the DNA-binding activity of a distinct factor, HSF1. This constitutes the first example of HSF2 activation in vivo. Both hemin and heat shock treatments resulted in equivalent levels of HSF-HSE complexes as analyzed in vitro by gel mobility shift assay, yet transcription of the hsp70 gene was stimulated much less by hemin-induced HSF than by heat shock-induced HSF. Genomic footprinting experiments revealed that hemin-induced HSF and heat shock-induced HSF, HSF2, and HSF1, respectively, occupy the HSE of the human hsp70 promoter in a similar yet not identical manner. We speculate that the difference in occupancy and/or in the transcriptional abilities of HSF1 and HSF2 accounts for the observed differences in the stimulation of hsp70 gene transcription.

Mol Cell Biol. 1992 September; 12(9): 4104-4111




This article has been cited by other articles:

  • Tateishi, Y., Ariyoshi, M., Igarashi, R., Hara, H., Mizuguchi, K., Seto, A., Nakai, A., Kokubo, T., Tochio, H., Shirakawa, M. (2009). Molecular Basis for SUMOylation-dependent Regulation of DNA Binding Activity of Heat Shock Factor 2. J. Biol. Chem. 284: 2435-2447 [Abstract] [Full Text]  
  • Fujimoto, M., Oshima, K., Shinkawa, T., Wang, B. B., Inouye, S., Hayashida, N., Takii, R., Nakai, A. (2008). Analysis of HSF4 Binding Regions Reveals Its Necessity for Gene Regulation during Development and Heat Shock Response in Mouse Lenses. J. Biol. Chem. 283: 29961-29970 [Abstract] [Full Text]  
  • Morimoto, R. I. (2008). Proteotoxic stress and inducible chaperone networks in neurodegenerative disease and aging. Genes Dev. 22: 1427-1438 [Abstract] [Full Text]  
  • Ostling, P., Bjork, J. K., Roos-Mattjus, P., Mezger, V., Sistonen, L. (2007). Heat Shock Factor 2 (HSF2) Contributes to Inducible Expression of hsp Genes through Interplay with HSF1. J. Biol. Chem. 282: 7077-7086 [Abstract] [Full Text]  
  • Shabtay, A., Arad, Z. (2006). Reciprocal activation of HSF1 and HSF3 in brain and blood tissues: is redundancy developmentally related?. Am. J. Physiol. Regul. Integr. Comp. Physiol. 291: R566-R572 [Abstract] [Full Text]  
  • Chang, Y., Ostling, P., Akerfelt, M., Trouillet, D., Rallu, M., Gitton, Y., El Fatimy, R., Fardeau, V., Le Crom, S., Morange, M., Sistonen, L., Mezger, V. (2006). Role of heat-shock factor 2 in cerebral cortex formation and as a regulatorof p35 expression.. Genes Dev. 20: 836-847 [Abstract] [Full Text]  
  • Tessari, A., Salata, E., Ferlin, A., Bartoloni, L., Slongo, M.L., Foresta, C. (2004). Characterization of HSFY, a novel AZFb gene on the Y chromosome with a possible role in human spermatogenesis. Mol Hum Reprod 10: 253-258 [Abstract] [Full Text]  
  • Alastalo, T.-P., Hellesuo, M., Sandqvist, A., Hietakangas, V., Kallio, M., Sistonen, L. (2003). Formation of nuclear stress granules involves HSF2 and coincides with the nucleolar localization of Hsp70. J. Cell Sci. 116: 3557-3570 [Abstract] [Full Text]  
  • McMillan, D. R., Christians, E., Forster, M., Xiao, X., Connell, P., Plumier, J.-C., Zuo, X., Richardson, J., Morgan, S., Benjamin, I. J. (2002). Heat Shock Transcription Factor 2 Is Not Essential for Embryonic Development, Fertility, or Adult Cognitive and Psychomotor Function in Mice. Mol. Cell. Biol. 22: 8005-8014 [Abstract] [Full Text]  
  • Tachibana, T., Astumi, S., Shioda, R., Ueno, M., Uritani, M., Ushimaru, T. (2002). A Novel Non-conventional Heat Shock Element Regulates Expression of MDJ1 Encoding a DnaJ Homolog in Saccharomyces cerevisiae. J. Biol. Chem. 277: 22140-22146 [Abstract] [Full Text]  
  • Mathew, A., Mathur, S. K., Jolly, C., Fox, S. G., Kim, S., Morimoto, R. I. (2001). Stress-Specific Activation and Repression of Heat Shock Factors 1 and 2. Mol. Cell. Biol. 21: 7163-7171 [Abstract] [Full Text]  
  • Ahn, S.-G., Liu, P. C.C., Klyachko, K., Morimoto, R. I., Thiele, D. J. (2001). The loop domain of heat shock transcription factor 1 dictates DNA-binding specificity and responses to heat stress. Genes Dev. 15: 2134-2145 [Abstract] [Full Text]  
  • Fishelson, Z., Hochman, I., Greene, L. E., Eisenberg, E. (2001). Contribution of heat shock proteins to cell protection from complement-mediated lysis. Int Immunol 13: 983-991 [Abstract] [Full Text]  
  • PIRKKALA, L., NYKANEN, P., SISTONEN, L. (2001). Roles of the heat shock transcription factors in regulation of the heat shock response and beyond. FASEB J. 15: 1118-1131 [Abstract] [Full Text]  
  • Kishida, A., Matsuyama, T., Nakashima, M., Serizawa, T., Akashi, M., Sugimura, K. (2000). Study of Cell-Lipid Film Interaction by Measuring Heat Shock Proteins mRNA Expression. Journal of Bioactive and Compatible Polymers 15: 478-488 [Abstract]  
  • Pirkkala, L., Alastalo, T.-P., Zuo, X., Benjamin, I. J., Sistonen, L. (2000). Disruption of Heat Shock Factor 1 Reveals an Essential Role in the Ubiquitin Proteolytic Pathway. Mol. Cell. Biol. 20: 2670-2675 [Abstract] [Full Text]  
  • Sun, L., Chang, J., Kirchhoff, S. R., Knowlton, A. A. (2000). Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment. Am. J. Physiol. Heart Circ. Physiol. 278: H1091-H1097 [Abstract] [Full Text]  
  • Kondo, T., Matsuda, T., Tashima, M., Umehara, H., Domae, N., Yokoyama, K., Uchiyama, T., Okazaki, T. (2000). Suppression of Heat Shock Protein-70 by Ceramide in Heat Shock-induced HL-60 Cell Apoptosis. J. Biol. Chem. 275: 8872-8879 [Abstract] [Full Text]  
  • Rabergh, C., Airaksinen, S, Soitamo, A, Bjorklund, H., Johansson, T, Nikinmaa, M, Sistonen, L (2000). Tissue-specific expression of zebrafish (Danio rerio) heat shock factor 1 mRNAs in response to heat stress. J. Exp. Biol. 203: 1817-1824 [Abstract]  
  • Ivaska, J., Reunanen, H., Westermarck, J., Koivisto, L., Kahari, V.-M., Heino, J. (1999). Integrin {alpha}2{beta}1 Mediates Isoform-Specific Activation of p38 and Upregulation of Collagen Gene Transcription by a Mechanism Involving the {alpha}2 Cytoplasmic Tail. JCB 147: 401-416 [Abstract] [Full Text]  
  • Tanabe, M., Sasai, N., Nagata, K., Liu, X.-D., Liu, P. C. C., Thiele, D. J., Nakai, A. (1999). The Mammalian HSF4 Gene Generates Both an Activator and a Repressor of Heat Shock Genes by Alternative Splicing. J. Biol. Chem. 274: 27845-27856 [Abstract] [Full Text]  
  • van Why, S. K., Kim, S., Geibel, J., Seebach, F. A., Kashgarian, M., Siegel, N. J. (1999). Thresholds for cellular disruption and activation of the stress response in renal epithelia. Am. J. Physiol. Renal Physiol. 277: F227-F234 [Abstract] [Full Text]  
  • Loflin, P., Chen, C.-Y. A., Shyu, A.-B. (1999). Unraveling a cytoplasmic role for hnRNP D in the in vivo mRNA destabilization directed by the AU-rich element. Genes Dev. 13: 1884-1897 [Abstract] [Full Text]  
  • PIRKKALA, L., ALASTALO, T.-P., NYKÄNEN, P., SEPPÄ, L., SISTONEN, L. (1999). Differentiation lineage-specific expression of human heat shock transcription factor 2. FASEB J. 13: 1089-1098 [Abstract] [Full Text]  
  • Hong, Y., Sarge, K. D. (1999). Regulation of Protein Phosphatase 2A Activity by Heat Shock Transcription Factor 2. J. Biol. Chem. 274: 12967-12970 [Abstract] [Full Text]  
  • Nishizawa, J., Nakai, A., Matsuda, K., Komeda, M., Ban, T., Nagata, K. (1999). Reactive Oxygen Species Play an Important Role in the Activation of Heat Shock Factor 1 in Ischemic-Reperfused Heart. Circulation 99: 934-941 [Abstract] [Full Text]  
  • Mercier, P., Winegarden, N., Westwood, J. (1999). Human heat shock factor 1 is predominantly a nuclear protein before and after heat stress. J. Cell Sci. 112: 2765-2774 [Abstract]  
  • Morimoto, R. I. (1998). Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev. 12: 3788-3796 [Full Text]  
  • Zhang, Y., Koushik, S., Dai, R., Mivechi, N. F. (1998). Structural Organization and Promoter Analysis of Murine Heat Shock Transcription Factor-1 Gene. J. Biol. Chem. 273: 32514-32521 [Abstract] [Full Text]  
  • Santoro, N., Johansson, N., Thiele, D. J. (1998). Heat Shock Element Architecture Is an Important Determinant in the Temperature and Transactivation Domain Requirements for Heat Shock Transcription Factor. Mol. Cell. Biol. 18: 6340-6352 [Abstract] [Full Text]  
  • Yoshima, T., Yura, T., Yanagi, H. (1998). Heat Shock Factor 1 Mediates Hemin-induced hsp70 Gene Transcription in K562 Erythroleukemia Cells. J. Biol. Chem. 273: 25466-25471 [Abstract] [Full Text]  
  • Mathew, A., Mathur, S. K., Morimoto, R. I. (1998). Heat Shock Response and Protein Degradation: Regulation of HSF2 by the Ubiquitin-Proteasome Pathway. Mol. Cell. Biol. 18: 5091-5098 [Abstract] [Full Text]  
  • Maheswaran, S., Englert, C., Zheng, G., Lee, S. B., Wong, J., Harkin, D. P., Bean, J., Ezzell, R., Garvin, A. J., McCluskey, R. T., DeCaprio, J. A., Haber, D. A. (1998). Inhibition of cellular proliferation by the Wilms tumor suppressor WT1 requires association with the inducible chaperone Hsp70. Genes Dev. 12: 1108-1120 [Abstract] [Full Text]  
  • Scharf, K.-D., Heider, H., Höhfeld, I., Lyck, R., Schmidt, E., Nover, L. (1998). The Tomato Hsf System: HsfA2 Needs Interaction with HsfA1 for Efficient Nuclear Import and May Be Localized in Cytoplasmic Heat Stress Granules. Mol. Cell. Biol. 18: 2240-2251 [Abstract] [Full Text]  
  • Kaarniranta, K., Elo, M., Sironen, R., Lammi, M. J., Goldring, M. B., Eriksson, J. E., Sistonen, L., Helminen, H. J. (1998). Hsp70 accumulation in chondrocytic cells exposed to high continuous hydrostatic pressure coincides with mRNA stabilization rather than transcriptional activation. Proc. Natl. Acad. Sci. USA 95: 2319-2324 [Abstract] [Full Text]  
  • Leppa, S., Pirkkala, L., Chow, S. C., Eriksson, J. E., Sistonen, L. (1997). Thioredoxin Is Transcriptionally Induced upon Activation of Heat Shock Factor 2. J. Biol. Chem. 272: 30400-30404 [Abstract] [Full Text]  
  • Leppa, S., Pirkkala, L., Saarento, H., Sarge, K. D., Sistonen, L. (1997). Overexpression of HSF2-beta Inhibits Hemin-induced Heat Shock Gene Expression and Erythroid Differentiation in K562 Cells. J. Biol. Chem. 272: 15293-15298 [Abstract] [Full Text]  
  • Tanabe, M., Nakai, A., Kawazoe, Y., Nagata, K. (1997). Different Thresholds in the Responses of Two Heat Shock Transcription Factors, HSF1 and HSF3. J. Biol. Chem. 272: 15389-15395 [Abstract] [Full Text]  
  • Mercier, P. A., Foksa, J., Ovsenek, N., Westwood, J. T. (1997). Xenopus Heat Shock Factor 1 Is a Nuclear Protein before Heat Stress. J. Biol. Chem. 272: 14147-14151 [Abstract] [Full Text]  
  • Rallu, M., Loones, Mt., Lallemand, Y., Morimoto, R., Morange, M., Mezger, V. (1997). Function and regulation of heat shock factor 2 during mouse embryogenesis. Proc. Natl. Acad. Sci. USA 94: 2392-2397 [Abstract] [Full Text]  
  • Holmberg, C. I., Leppa, S., Eriksson, J. E., Sistonen, L. (1997). The Phorbol Ester 12-O-Tetradecanoylphorbol 13-Acetate Enhances the Heat-induced Stress Response. J. Biol. Chem. 272: 6792-6798 [Abstract] [Full Text]  
  • Cullen, K. E., Sarge, K. D. (1997). Characterization of Hypothermia-induced Cellular Stress Response in Mouse Tissues. J. Biol. Chem. 272: 1742-1746 [Abstract] [Full Text]  
  • Cotto, J, Fox, S, Morimoto, R (1997). HSF1 granules: a novel stress-induced nuclear compartment of human cells. J. Cell Sci. 110: 2925-2934 [Abstract]  
  • Rossi, A., Elia, G., Santoro, M. G. (1996). 2-Cyclopenten-1-one, a New Inducer of Heat Shock Protein 70with Antiviral Activity. J. Biol. Chem. 271: 32192-32196 [Abstract] [Full Text]  
  • Knauf, U, Newton, E M, Kyriakis, J, Kingston, R E (1996). Repression of human heat shock factor 1 activity at control temperature by phosphorylation.. Genes Dev. 10: 2782-2793 [Abstract]  
  • Nishizawa, J., Nakai, A., Higashi, T., Tanabe, M., Nomoto, S., Matsuda, K., Ban, T., Nagata, K. (1996). Reperfusion Causes Significant Activation of Heat Shock Transcription Factor 1 in Ischemic Rat Heart. Circulation 94: 2185-2192 [Abstract] [Full Text]  
  • Elia, G., De Marco, A., Rossi, A., Santoro, M. G. (1996). Inhibition of HSP70 Expression by Calcium Ionophore A23187 in Human Cells. AN EFFECT INDEPENDENT OF THE ACQUISITION OF DNA-BINDING ACTIVITY BY THE HEAT SHOCK TRANSCRIPTION FACTOR. J. Biol. Chem. 271: 16111-16118 [Abstract] [Full Text]  
  • Cotto, J. J., Kline, M., Morimoto, R. I. (1996). Activation of Heat Shock Factor 1 DNA Binding Precedes Stress-induced Serine Phosphorylation. J. Biol. Chem. 271: 3355-3358 [Abstract] [Full Text]  
  • Gorzowski, J. J., Eckerley, C. A., Halgren, R. G., Mangurten, A. B., Phillips, B. (1995). Methylation-associated Transcriptional Silencing of the Major Histocompatibility Complex-linked hsp70 Genes in Mouse Cell Lines. J. Biol. Chem. 270: 26940-26949 [Abstract] [Full Text]  
  • Jurivich, D. A., Pachetti, C., Qiu, L., Welk, J. F. (1995). Salicylate Triggers Heat Shock Factor Differently than Heat. J. Biol. Chem. 270: 24489-24495 [Abstract] [Full Text]  
  • Minowada, G., Welch, W. (1995). Variation in the Expression and/or Phosphorylation of the Human Low Molecular Weight Stress Protein during in Vitro Cell Differentiation. J. Biol. Chem. 270: 7047-7054 [Abstract] [Full Text]  
  • Sheldon, L A, Kingston, R E (1993). Hydrophobic coiled-coil domains regulate the subcellular localization of human heat shock factor 2.. Genes Dev. 7: 1549-1558 [Abstract]  
  • Morimoto, R. (1993). Cells in stress: transcriptional activation of heat shock genes. Science 259: 1409-1410  
  • Leppa, S., Kajanne, R., Arminen, L., Sistonen, L. (2001). Differential Induction of Hsp70-encoding Genes in Human Hematopoietic Cells. J. Biol. Chem. 276: 31713-31719 [Abstract] [Full Text]  
  • Yet, S.-F., Tian, R., Layne, M. D., Wang, Z. Y., Maemura, K., Solovyeva, M., Ith, B., Melo, L. G., Zhang, L., Ingwall, J. S., Dzau, V. J., Lee, M.-E., Perrella, M. A. (2001). Cardiac-Specific Expression of Heme Oxygenase-1 Protects Against Ischemia and Reperfusion Injury in Transgenic Mice. Circ. Res. 89: 168-173 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»