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Mol Cell Biol. 1993 January; 13(1): 668-676

GATA and Ets cis-acting sequences mediate megakaryocyte-specific expression.

V Lemarchandel, J Ghysdael, V Mignotte, C Rahuel and P H Roméo

INSERM U.91, Hôpital Henri-Mondor, Creteil, France.

ABSTRACT

The human glycoprotein IIB (GPIIB) gene is expressed only in megakaryocytes, and its promoter displays cell type specificity. We show that this specificity involved two cis-acting sequences. The first one, located at -55, contains a GATA binding site. Point mutations that abolish protein binding on this site decrease the activity of the GPIIB promoter but do not affect its tissue specificity. The second one, located at -40, contains an Ets consensus sequence, and we show that Ets-1 or Ets-2 protein can interact with this -40 GPIIB sequence. Point mutations that impair Ets binding decrease the activity of the GPIIB promoter to the same extent as do mutations that abolish GATA binding. A GPIIB 40-bp DNA fragment containing the GATA and Ets binding sites can confer activity to a heterologous promoter in megakaryocytic cells. This activity is independent of the GPIIB DNA fragment orientation, and mutations on each binding site result in decreased activity. Using cotransfection assays, we show that c-Ets-1 and human GATA1 can transactive the GPIIB promoter in HeLa cells and can act additively. Northern (RNA) blot analysis indicates that the ets-1 mRNA level is increased during megakaryocyte-induced differentiation of erythrocytic/megakaryocytic cell lines. Gel retardation assays show that the same GATA-Ets association is found in the human GPIIB enhancer and the rat platelet factor 4 promoter, the other two characterized regulatory regions of megakaryocyte-specific genes. These results indicate that GATA and Ets cis-acting sequences are an important determinant of megakaryocytic specific gene expression.

Mol Cell Biol. 1993 January; 13(1): 668-676




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  • Hashimoto, Y., Ware, J. (1995). Identification of Essential GATA and Ets Binding Motifs within the Promoter of the Platelet Glycoprotein Ibalpha Gene. J. Biol. Chem. 270: 24532-24539 [Abstract] [Full Text]  
  • Kulessa, H, Frampton, J, Graf, T (1995). GATA-1 reprograms avian myelomonocytic cell lines into eosinophils, thromboblasts, and erythroblasts.. Genes Dev. 9: 1250-1262 [Abstract]  
  • Vieille-Grosjean, I., Huber, P. (1995). Transcription Factor GATA-1 Regulates Human HOXB2 Gene Expression in Erythroid Cells. J. Biol. Chem. 270: 4544-4550 [Abstract] [Full Text]  
  • Avraham, S., Jiang, S., Ota, S., Fu, Y., Deng, B., Dowler, L. L., White, R. A., Avraham, H. (1995). Structural and Functional Studies of the Intracellular Tyrosine Kinase MATK Gene and Its Translated Product. J. Biol. Chem. 270: 1833-1842 [Abstract] [Full Text]  
  • Pevny, L, Lin, C., D'Agati, V, Simon, M., Orkin, S., Costantini, F (1995). Development of hematopoietic cells lacking transcription factor GATA-1. Development 121: 163-172 [Abstract]  
  • Racke, F. K., Wang, D., Zaidi, Z., Kelley, J., Visvader, J., Soh, J.-W., Goldfarb, A. N. (2001). A Potential Role for Protein Kinase C-epsilon in Regulating Megakaryocytic Lineage Commitment. J. Biol. Chem. 276: 522-528 [Abstract] [Full Text]  
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  • Schick, B. P., Petrushina, I., Brodbeck, K. C., Castronuevo, P. (2001). Promoter Regulatory Elements and DNase I-hypersensitive Sites Involved in Serglycin Proteoglycan Gene Expression in Human Erythroleukemia, CHRF 288-11, and HL-60 Cells. J. Biol. Chem. 276: 24726-24735 [Abstract] [Full Text]  
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