This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ryan, J J
Right arrow Articles by Clarke, M F
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ryan, J J
Right arrow Articles by Clarke, M F

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 January; 13(1): 711-719

Cell cycle analysis of p53-induced cell death in murine erythroleukemia cells.

J J Ryan, R Danish, C A Gottlieb and M F Clarke

Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0668.

ABSTRACT

A temperature-sensitive mutant of murine p53 (p53Val-135) was transfected by electroporation into murine erythroleukemia cells (DP16-1) lacking endogenous expression of p53. While the transfected cells grew normally in the presence of mutant p53 (37.5 degrees C), wild-type p53 (32.5 degrees C) was associated with a rapid loss of cell viability. Genomic DNA extracted at 32.5 degrees C was seen to be fragmented into a characteristic ladder consistent with cell death due to apoptosis. Following synchronization by density arrest, transfected cells released into G1 at 32.5 degrees C were found to lose viability more rapidly than did randomly growing cultures. Following release into G1, cells became irreversibly committed to cell death after 4 h at 32.5 degrees C. Commitment to cell death correlated with the first appearance of fragmented DNA. Synchronized cells allowed to pass out of G1 prior to being placed at 32.5 degrees C continued to cycle until subsequently arrested in G1; loss of viability occurred following G1 arrest. In contrast to cells in G1, cells cultured at 32.5 degrees C for prolonged periods during S phase and G2/M, and then returned to 37.5 degrees C, did not become committed to cell death. G1 arrest at 37.5 degrees C, utilizing either mimosine or isoleucine deprivation, does not lead to rapid cell death. Upon transfer to 32.5 degrees C, these G1 synchronized cell populations quickly lost viability. Cells that were kept density arrested at 32.5 degrees C (G0) lost viability at a much slower rate than did cells released into G1. Taken together, these results indicate that wild-type p53 induces cell death in murine erythroleukemia cells and that this effect occurs predominantly in the G1 phase of actively cycling cells.

Mol Cell Biol. 1993 January; 13(1): 711-719




This article has been cited by other articles:

  • Shamhart, P. E., Luther, D. J., Hodson, B. R., Koshy, J. C., Ohanyan, V., Meszaros, J. G. (2009). Impact of type 1 diabetes on cardiac fibroblast activation: enhanced cell cycle progression and reduced myofibroblast content in diabetic myocardium. Am. J. Physiol. Endocrinol. Metab. 297: E1147-E1153 [Abstract] [Full Text]  
  • Arce, S., Nawar, H. F., Russell, M. W., Connell, T. D. (2005). Differential Binding of Escherichia coli Enterotoxins LT-IIa and LT-IIb and of Cholera Toxin Elicits Differences in Apoptosis, Proliferation, and Activation of Lymphoid Cells. Infect. Immun. 73: 2718-2727 [Abstract] [Full Text]  
  • Vaghefi, H., Hughes, A. L., Neet, K. E. (2004). Nerve Growth Factor Withdrawal-mediated Apoptosis in Naive and Differentiated PC12 Cells through p53/Caspase-3-dependent and -independent Pathways. J. Biol. Chem. 279: 15604-15614 [Abstract] [Full Text]  
  • Vitale, M., Di Matola, T., D'Ascoli, F., Salzano, S., Bogazzi, F., Fenzi, G., Martino, E., Rossi, G. (2000). Iodide Excess Induces Apoptosis in Thyroid Cells through a p53-Independent Mechanism Involving Oxidative Stress. Endocrinology 141: 598-605 [Abstract] [Full Text]  
  • NASREEN, N., MOHAMMED, K. A., DOWLING, P. A., WARD, M. J., GALFFY, G., ANTONY, V. B. (2000). Talc Induces Apoptosis in Human Malignant Mesothelioma Cells In Vitro. Am. J. Respir. Crit. Care Med. 161: 595-600 [Abstract] [Full Text]  
  • Devireddy, L. R., Jones, C. J. (1999). Activation of Caspases and p53 by Bovine Herpesvirus 1 Infection Results in Programmed Cell Death and Efficient Virus Release. J. Virol. 73: 3778-3788 [Abstract] [Full Text]  
  • Tessitore, L., Sesca, E., Bosco, M., Vance, D. E. (1999). Expression of phosphatidylethanolamine N-methyltransferase in Yoshida ascites hepatoma cells and the livers of host rats. Carcinogenesis 20: 561-567 [Abstract] [Full Text]  
  • Faderl, S., Kantarjian, H. M., Talpaz, M., Estrov, Z. (1998). Clinical Significance of Cytogenetic Abnormalities in Adult Acute Lymphoblastic Leukemia. Blood 91: 3995-4019 [Full Text]  
  • Kojima, H., Tanaka, Y., Tanaka, T., Miyazaki, H., Shiwa, M., Kamide, Y., Moriyama, H. (1998). Cell Proliferation and Apoptosis in Human Middle Ear Cholesteatoma. Arch Otolaryngol Head Neck Surg 124: 261-264 [Abstract] [Full Text]  
  • Moll, U.M., Schramm, L.M. (1998). p53--An Acrobat in Tumorigenesis. CROBM 9: 23-37 [Abstract] [Full Text]  
  • Koury, M. J., Horne, D. W., Brown, Z. A., Pietenpol, J. A., Blount, B. C., Ames, B. N., Hard, R., Koury, S. T. (1997). Apoptosis of Late-Stage Erythroblasts in Megaloblastic Anemia: Association With DNA Damage and Macrocyte Production. Blood 89: 4617-4623 [Abstract] [Full Text]  
  • Lee, H., Larner, J. M., Hamlin, J. L. (1997). A p53-independent damage-sensing mechanism that functions as a checkpoint at the G1/S transition in Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 94: 526-531 [Abstract] [Full Text]  
  • Ko, L J, Prives, C (1996). p53: puzzle and paradigm.. Genes Dev. 10: 1054-1072  
  • Meikrantz, W., Schlegel, R. (1996). Suppression of Apoptosis by Dominant Negative Mutants of Cyclin-dependent Protein Kinases. J. Biol. Chem. 271: 10205-10209 [Abstract] [Full Text]  
  • Haupt, Y, Rowan, S, Shaulian, E, Vousden, K H, Oren, M (1995). Induction of apoptosis in HeLa cells by trans-activation-deficient p53.. Genes Dev. 9: 2170-2183 [Abstract]  
  • Gilbert, D. M., Neilson, A., Miyazawa, H., DePamphilis, M. L., Burhans, W. C. (1995). Mimosine Arrests DNA Synthesis at Replication Forks by Inhibiting Deoxyribonucleotide Metabolism. J. Biol. Chem. 270: 9597-9606 [Abstract] [Full Text]  
  • Di Leonardo, A, Linke, S P, Clarkin, K, Wahl, G M (1994). DNA damage triggers a prolonged p53-dependent G1 arrest and long-term induction of Cip1 in normal human fibroblasts.. Genes Dev. 8: 2540-2551 [Abstract]  
  • Gonzalez-Garcia, M, Perez-Ballestero, R, Ding, L, Duan, L, Boise, L., Thompson, C., Nunez, G (1994). bcl-XL is the major bcl-x mRNA form expressed during murine development and its product localizes to mitochondria. Development 120: 3033-3042 [Abstract]  
  • Cory, S., Strasser, A., Jacks, T., Corcoran, L.M., Metz, T., Harris, A.W., Adams, J.M. (1994). Enhanced Cell Survival and Tumorigenesis. Cold Spring Harb Symp Quant Biol 59: 365-375 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»