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Mol Cell Biol. 1993 October; 13(10): 5981-5989

ADA3: a gene, identified by resistance to GAL4-VP16, with properties similar to and different from those of ADA2.

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

ABSTRACT

We describe the isolation of a yeast gene, ADA3, mutations in which prevent the toxicity of GAL4-VP16 in vivo. Toxicity was previously proposed to be due to the trapping of general transcription factors required at RNA polymerase II promoters (S. L. Berger, B. Piña, N. Silverman, G. A. Marcus, J. Agapite, J. L. Regier, S. J. Triezenberg, and L. Guarente, Cell 70:251-265, 1992). trans activation by VP16 as well as the acidic activation domain of GCN4 is reduced in the mutant. Other activation domains, such as those of GAL4 and HAP4, are only slightly affected in the mutant. This spectrum is similar to that observed for mutants with lesions in ADA2, a gene proposed to encode a transcriptional adaptor. The ADA3 gene is not absolutely essential for cell growth, but gene disruption mutants grow slowly and are temperature sensitive. Strains doubly disrupted for ada2 and ada3 grow no more slowly than single mutants, providing further evidence that these genes function in the same pathway. Selection of initiation sites by the general transcriptional machinery in vitro is altered in the ada3 mutant, providing a clue that ADA3 could be a novel general transcription factor involved in the response to acidic activators.

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• Gancedo, J. M. (1998). Yeast Carbon Catabolite Repression. Microbiol. Mol. Biol. Rev. 62: 334-361 [Abstract] [Full Text]  
• Hampsey, M. (1998). Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery. Microbiol. Mol. Biol. Rev. 62: 465-503 [Abstract] [Full Text]  
• Lee, T. I., Young, R. A. (1998). Regulation of gene expression by TBP-associated proteins. Genes Dev. 12: 1398-1408 [Full Text]  
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• Drysdale, C. M., Jackson, B. M., McVeigh, R., Klebanow, E. R., Bai, Y., Kokubo, T., Swanson, M., Nakatani, Y., Weil, P. A., Hinnebusch, A. G. (1998). The Gcn4p Activation Domain Interacts Specifically In Vitro with RNA Polymerase II Holoenzyme, TFIID, and the Adap-Gcn5p Coactivator Complex. Mol. Cell. Biol. 18: 1711-1724 [Abstract] [Full Text]  
• Wang, L., Liu, L., Berger, S. L. (1998). Critical residues for histone acetylation by Gcn5, functioning in Ada and SAGA complexes, are also required for transcriptional function in vivo. Genes Dev. 12: 640-653 [Abstract] [Full Text]  
• Grant, P A, Duggan, L, Cote, J, Roberts, S M, Brownell, J E, Candau, R, Ohba, R, Owen-Hughes, T, Allis, C D, Winston, F, Berger, S L, Workman, J L (1997). Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex.. Genes Dev. 11: 1640-1650 [Abstract]  
• Saleh, A., Lang, V., Cook, R., Brandl, C. J. (1997). Identification of Native Complexes Containing the Yeast Coactivator/Repressor Proteins NGG1/ADA3 and ADA2. J. Biol. Chem. 272: 5571-5578 [Abstract] [Full Text]  
• Chiang, Y.-C., Komarnitsky, P., Chase, D., Denis, C. L. (1996). ADR1 Activation Domains Contact the Histone Acetyltransferase GCN5 and the Core Transcriptional Factor TFIIB. J. Biol. Chem. 271: 32359-32365 [Abstract] [Full Text]  
• Martens, J. A., Genereaux, J., Saleh, A., Brandl, C. J. (1996). Transcriptional Activation by Yeast PDR1p Is Inhibited by Its Association with NGG1p/ADA3p. J. Biol. Chem. 271: 15884-15890 [Abstract] [Full Text]  
• Brandl, C. J., Martens, J. A., Margaliot, A., Stenning, D., Furlanetto, A. M., Saleh, A., Hamilton, K. S., Genereaux, J. (1996). Structure/Function Properties of the Yeast Dual Regulator Protein NGG1 That Are Required for Glucose Repression. J. Biol. Chem. 271: 9298-9306 [Abstract] [Full Text]  
• Candau, R., Berger, S. L. (1996). Structural and Functional Analysis of Yeast Putative Adaptors. J. Biol. Chem. 271: 5237-5245 [Abstract] [Full Text]  
• Barlev, N. A., Candau, R., Wang, L., Darpino, P., Silverman, N., Berger, S. L. (1995). Characterization of Physical Interactions of the Putative Transcriptional Adaptor, ADA2, with Acidic Activation Domains and TATA-binding Protein. J. Biol. Chem. 270: 19337-19344 [Abstract] [Full Text]  
• Hengartner, C J, Thompson, C M, Zhang, J, Chao, D M, Liao, S M, Koleske, A J, Okamura, S, Young, R A (1995). Association of an activator with an RNA polymerase II holoenzyme.. Genes Dev. 9: 897-910 [Abstract]  
• Schmitz, M. L., Stelzer, G., Altmann, H., Meisterernst, M., Baeuerle, P. A. (1995). Interaction of the COOH-terminal Transactivation Domain of p65 NF-kappaB with TATA-binding Protein, Transcription Factor IIB, and Coactivators. J. Biol. Chem. 270: 7219-7226 [Abstract] [Full Text]