This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mougey, E B
Right arrow Articles by Sollner-Webb, B
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mougey, E B
Right arrow Articles by Sollner-Webb, B

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 October; 13(10): 5990-5998

A U3 small nuclear ribonucleoprotein-requiring processing event in the 5' external transcribed spacer of Xenopus precursor rRNA.

E B Mougey, L K Pape and B Sollner-Webb

Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

ABSTRACT

A processing site has been identified within the 5' external transcribed spacer (ETS) of Xenopus laevis and X. borealis pre-RNAs, and this in vivo processing can be reproduced in vitro. It involves a stable and specific association of the pre-rRNA with factors in the cell extract, including at least four RNA-contacting polypeptides, yielding a distinct complex that sediments at 20S. Processing also requires the U3 small nuclear RNA. This processing, at residue +105 of the 713-nucleotide X. laevis 5' ETS, is highly reminiscent of the initial processing cleavage of mouse pre-rRNA within its 3.5-kb 5' ETS, previously thought to be mammal specific. The frog and mouse processing signals share a short essential sequence motif, and mouse factors can faithfully process the frog pre-rRNA. This conservation suggests that this 5' ETS processing site serves an evolutionarily selective function.

Mol Cell Biol. 1993 October; 13(10): 5990-5998




This article has been cited by other articles:

  • Champion, E. A., Lane, B. H., Jackrel, M. E., Regan, L., Baserga, S. J. (2008). A Direct Interaction between the Utp6 Half-a-Tetratricopeptide Repeat Domain and a Specific Peptide in Utp21 Is Essential for Efficient Pre-rRNA Processing. Mol. Cell. Biol. 28: 6547-6556 [Abstract] [Full Text]  
  • Saez-Vasquez, J., Caparros-Ruiz, D., Barneche, F., Echeverria, M. (2004). A Plant snoRNP Complex Containing snoRNAs, Fibrillarin, and Nucleolin-Like Proteins Is Competent for both rRNA Gene Binding and Pre-rRNA Processing In Vitro. Mol. Cell. Biol. 24: 7284-7297 [Abstract] [Full Text]  
  • BOROVJAGIN, A. V., GERBI, S. A. (2004). Xenopus U3 snoRNA docks on pre-rRNA through a novel base-pairing interaction. RNA 10: 942-953 [Abstract] [Full Text]  
  • Atzorn, V., Fragapane, P., Kiss, T. (2004). U17/snR30 Is a Ubiquitous snoRNA with Two Conserved Sequence Motifs Essential for 18S rRNA Production. Mol. Cell. Biol. 24: 1769-1778 [Abstract] [Full Text]  
  • Jin, S.-B., Zhao, J., Bjork, P., Schmekel, K., Ljungdahl, Per. O., Wieslander, L. (2002). Mrd1p Is Required for Processing of Pre-rRNA and for Maintenance of Steady-state Levels of 40 S Ribosomal Subunits in Yeast. J. Biol. Chem. 277: 18431-18439 [Abstract] [Full Text]  
  • Borovjagin, A. V., Gerbi, S. A. (2001). Xenopus U3 snoRNA GAC-Box A' and Box A Sequences Play Distinct Functional Roles in rRNA Processing. Mol. Cell. Biol. 21: 6210-6221 [Abstract] [Full Text]  
  • Greenwood, S. J., Schnare, M. N., Cook, J. R., Gray, M. W. (2001). Analysis of intergenic spacer transcripts suggests 'read-around' transcription of the extrachromosomal circular rDNA in Euglena gracilis. Nucleic Acids Res 29: 2191-2198 [Abstract] [Full Text]  
  • GERBI, S.A., BOROVJAGIN, A.V., EZROKHI, M., LANGE, T.S. (2001). Ribosome Biogenesis: Role of Small Nucleolar RNA in Maturation of Eukaryotic rRNA. Cold Spring Harb Symp Quant Biol 66: 575-590 [Abstract]  
  • Colley, A., Beggs, J. D., Tollervey, D., Lafontaine, D. L. J. (2000). Dhr1p, a Putative DEAH-Box RNA Helicase, Is Associated with the Box C+D snoRNP U3. Mol. Cell. Biol. 20: 7238-7246 [Abstract] [Full Text]  
  • Schnare, M. N., Collings, J. C., Spencer, D. F., Gray, M. W. (2000). The 28S-18S rDNA intergenic spacer from Crithidia fasciculata: repeated sequences, length heterogeneity, putative processing sites and potential interactions between U3 small nucleolar RNA and the ribosomal RNA precursor. Nucleic Acids Res 28: 3452-3461 [Abstract] [Full Text]  
  • Lukowiak, A. A., Granneman, S., Mattox, S. A., Speckmann, W. A., Jones, K., Pluk, H., Venrooij, W. J. v., Terns, R. M., Terns, M. P. (2000). Interaction of the U3-55k protein with U3 snoRNA is mediated by the Box B/C motif of U3 and the WD repeats of U3-55k. Nucleic Acids Res 28: 3462-3471 [Abstract] [Full Text]  
  • Dunbar, D. A., Chen, A. A., Wormsley, S., Baserga, S. J. (2000). The genes for small nucleolar RNAs in Trypanosoma brucei are organized in clusters and are transcribed as a polycistronic RNA. Nucleic Acids Res 28: 2855-2861 [Abstract] [Full Text]  
  • Antal, M., Mougin, A., Kis, M., Boros, E., Steger, G., Jakab, G., Solymosy, F., Branlant, C. (2000). Molecular characterization at the RNA and gene levels of U3 snoRNA from a unicellular green alga, Chlamydomonas reinhardtii. Nucleic Acids Res 28: 2959-2968 [Abstract] [Full Text]  
  • Jady, B. E., Kiss, T. (2000). Characterisation of the U83 and U84 small nucleolar RNAs: two novel 2'-O-ribose methylation guide RNAs that lack complementarities to ribosomal RNAs. Nucleic Acids Res 28: 1348-1354 [Abstract] [Full Text]  
  • Speckmann, W., Narayanan, A., Terns, R., Terns, M. P. (1999). Nuclear Retention Elements of U3 Small Nucleolar RNA. Mol. Cell. Biol. 19: 8412-8421 [Abstract] [Full Text]  
  • Ginisty, H, Sicard, H, Roger, B, Bouvet, P (1999). Structure and functions of nucleolin. J. Cell Sci. 112: 761-772 [Abstract]  
  • Dundr, M., Olson, M. O.J. (1998). Partially Processed pre-rRNA Is Preserved in Association with Processing Components in Nucleolus-derived Foci during Mitosis. Mol. Biol. Cell 9: 2407-2422 [Abstract] [Full Text]  
  • Samarsky, D. A., Fournier, M. J. (1998). Functional Mapping of the U3 Small Nucleolar RNA from the Yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 18: 3431-3444 [Abstract] [Full Text]  
  • Lee, S. J., Baserga, S. J. (1997). Functional separation of pre-rRNA processing steps revealed by truncation of the U3 small nucleolar ribonucleoprotein component, Mpp10. Proc. Natl. Acad. Sci. USA 94: 13536-13541 [Abstract] [Full Text]  
  • Eckmann, C. R., Jantsch, M. F. (1997). Xlrbpa, a Double-stranded RNA-binding Protein Associated with Ribosomes and Heterogeneous Nuclear RNPs. JCB 138: 239-253 [Abstract] [Full Text]  
  • Ganot, P, Caizergues-Ferrer, M, Kiss, T (1997). The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation.. Genes Dev. 11: 941-956 [Abstract]  
  • Beven, A., Lee, R, Razaz, M, Leader, D., Brown, J., Shaw, P. (1996). The organization of ribosomal RNA processing correlates with the distribution of nucleolar snRNAs. J. Cell Sci. 109: 1241-1251 [Abstract]  
  • Liang, W Q, Fournier, M J (1995). U14 base-pairs with 18S rRNA: a novel snoRNA interaction required for rRNA processing.. Genes Dev. 9: 2433-2443 [Abstract]  
  • Tycowski, K., Shu, M., Steitz, J. (1994). Requirement for intron-encoded U22 small nucleolar RNA in 18S ribosomal RNA maturation. Science 266: 1558-1561 [Abstract]  
  • Ginisty, H., Serin, G., Ghisolfi-Nieto, L., Roger, B., Libante, V., Amalric, F., Bouvet, P. (2000). Interaction of Nucleolin with an Evolutionarily Conserved Pre-ribosomal RNA Sequence Is Required for the Assembly of the Primary Processing Complex. J. Biol. Chem. 275: 18845-18850 [Abstract] [Full Text]  
  • Ginisty, H., Amalric, F., Bouvet, P. (2001). Two Different Combinations of RNA-binding Domains Determine the RNA Binding Specificity of Nucleolin. J. Biol. Chem. 276: 14338-14343 [Abstract] [Full Text]  
  • Waggener, J. M., DiMario, P. J. (2002). Two Splice Variants of Nopp140 in Drosophila melanogaster. Mol. Biol. Cell 13: 362-381 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»