This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Boubnov, N V Articles by Weaver, D T Search for Related Content PubMed PubMed Citation Articles by Boubnov, N V Articles by Weaver, D T

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 November; 13(11): 6957-6968

V(D)J recombination coding junction formation without DNA homology: processing of coding termini.

Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts.

ABSTRACT

Coding junction formation in V(D)J recombination generates diversity in the antigen recognition structures of immunoglobulin and T-cell receptor molecules by combining processes of deletion of terminal coding sequences and addition of nucleotides prior to joining. We have examined the role of coding end DNA composition in junction formation with plasmid substrates containing defined homopolymers flanking the recombination signal sequence elements. We found that coding junctions formed efficiently with or without terminal DNA homology. The extent of junctional deletion was conserved independent of coding ends with increased, partial, or no DNA homology. Interestingly, G/C homopolymer coding ends showed reduced deletion regardless of DNA homology. Therefore, DNA homology cannot be the primary determinant that stabilizes coding end structures for processing and joining.

This article has been cited by other articles:

• Lieber, M. R., Raghavan, S. C., Yu, K. (2008). Mechanistic Aspects of Lymphoid Chromosomal Translocations. J Natl Cancer Inst Monogr 2008: 8-11 [Abstract] [Full Text]  
• Montalbano, A., Ogwaro, K. M., Tang, A., Matthews, A. G. W., Larijani, M., Oettinger, M. A., Feeney, A. J. (2003). V(D)J Recombination Frequencies Can Be Profoundly Affected by Changes in the Spacer Sequence. J. Immunol. 171: 5296-5304 [Abstract] [Full Text]  
• Nakajima, P. B., Bosma, M. J. (2002). Variable Diversity Joining Recombination: Nonhairpin Coding Ends in Thymocytes of SCID and Wild-Type Mice. J. Immunol. 169: 3094-3104 [Abstract] [Full Text]  
• Yu, K., Lieber, M. R. (1999). Mechanistic Basis for Coding End Sequence Effects in the Initiation of V(D)J Recombination. Mol. Cell. Biol. 19: 8094-8102 [Abstract] [Full Text]  
• Zhu, X., Boonthum, A., Zhai, S.-K., Knight, K. L. (1999). B Lymphocyte Selection and Age-Related Changes in VH Gene Usage in Mutant Alicia Rabbits. J. Immunol. 163: 3313-3320 [Abstract] [Full Text]  
• Kurimasa, A., Kumano, S., Boubnov, N. V., Story, M. D., Tung, C.-S., Peterson, S. R., Chen, D. J. (1999). Requirement for the Kinase Activity of Human DNA-Dependent Protein Kinase Catalytic Subunit in DNA Strand Break Rejoining. Mol. Cell. Biol. 19: 3877-3884 [Abstract] [Full Text]  
• Bentolila, L. A., Olson, S., Marshall, A., Rougeon, F., Paige, C. J., Doyen, N., Wu, G. E. (1999). Extensive Junctional Diversity in Ig Light Chain Genes from Early B Cell Progenitors of {micro}MT Mice. J. Immunol. 162: 2123-2128 [Abstract] [Full Text]  
• Nadel, B., Tang, A., Escuro, G., Lugo, G., Feeney, A. J. (1998). Sequence of the Spacer in the Recombination Signal Sequence Affects V(D)J Rearrangement Frequency and Correlates with Nonrandom Vkappa Usage In Vivo. J. Exp. Med. 187: 1495-1503 [Abstract] [Full Text]  
• Sadofsky, M J, Hesse, J E, van Gent, D C, Gellert, M (1995). RAG-1 mutations that affect the target specificity of V(D)j recombination: a possible direct role of RAG-1 in site recognition.. Genes Dev. 9: 2193-2199 [Abstract]