This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rivera, R R
Right arrow Articles by Murre, C
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rivera, R R
Right arrow Articles by Murre, C

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 November; 13(11): 7163-7169

Ets proteins: new factors that regulate immunoglobulin heavy-chain gene expression.

R R Rivera, M H Stuiver, R Steenbergen and C Murre

Department of Biology, University of California, San Diego, La Jolla 92122.

ABSTRACT

We used a DNA-protein interaction screening method to isolate a cDNA, Erg-3, whose product binds to a site, designated pi, present in the immunoglobulin (Ig) heavy-chain gene enhancer. Erg-3 is an alternatively spliced product of the erg gene and contains an Ets DNA-binding domain. Fli-1 and PU.1, related Ets proteins, also bind to the same site. In addition, PU.1 binds to a second site, designated microB, in the Ig heavy-chain enhancer. We demonstrate that the pi binding site is crucial for Ig heavy-chain gene enhancer function. In addition, we show that Erg-3 and Fli.1, but not PU.1, can activate a reporter construct containing a multimer of protein-binding sites, synergistically with helix-loop-helix protein E12. We discuss how combinatorial interactions between members of the helix-loop-helix and Ets families may account for the tissue specificity of these proteins.

Mol Cell Biol. 1993 November; 13(11): 7163-7169




This article has been cited by other articles:

  • Kruse, E. A., Loughran, S. J., Baldwin, T. M., Josefsson, E. C., Ellis, S., Watson, D. K., Nurden, P., Metcalf, D., Hilton, D. J., Alexander, W. S., Kile, B. T. (2009). Dual requirement for the ETS transcription factors Fli-1 and Erg in hematopoietic stem cells and the megakaryocyte lineage. Proc. Natl. Acad. Sci. USA 106: 13814-13819 [Abstract] [Full Text]  
  • Schweitzer, B. L., Huang, K. J., Kamath, M. B., Emelyanov, A. V., Birshtein, B. K., DeKoter, R. P. (2006). Spi-C Has Opposing Effects to PU.1 on Gene Expression in Progenitor B Cells. J. Immunol. 177: 2195-2207 [Abstract] [Full Text]  
  • van Zelm, M. C., van der Burg, M., de Ridder, D., Barendregt, B. H., de Haas, E. F. E., Reinders, M. J. T., Lankester, A. C., Revesz, T., Staal, F. J. T., van Dongen, J. J. M. (2005). Ig Gene Rearrangement Steps Are Initiated in Early Human Precursor B Cell Subsets and Correlate with Specific Transcription Factor Expression. J. Immunol. 175: 5912-5922 [Abstract] [Full Text]  
  • Back, J., Dierich, A., Bronn, C., Kastner, P., Chan, S. (2004). PU.1 determines the self-renewal capacity of erythroid progenitor cells. Blood 103: 3615-3623 [Abstract] [Full Text]  
  • Nagulapalli, S., Goheer, A., Pitt, L., McIntosh, L. P., Atchison, M. L. (2002). Mechanism of E47-Pip Interaction on DNA Resulting in Transcriptional Synergy and Activation of Immunoglobulin Germ Line Sterile Transcripts. Mol. Cell. Biol. 22: 7337-7350 [Abstract] [Full Text]  
  • Jundt, F., Kley, K., Anagnostopoulos, I., Schulze Probsting, K., Greiner, A., Mathas, S., Scheidereit, C., Wirth, T., Stein, H., Dorken, B. (2002). Loss of PU.1 expression is associated with defective immunoglobulin transcription in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease. Blood 99: 3060-3062 [Abstract] [Full Text]  
  • Torlakovic, E., Tierens, A., Dang, H. D., Delabie, J. (2001). The Transcription Factor PU.1, Necessary for B-Cell Development Is Expressed in Lymphocyte Predominance, But Not Classical Hodgkin's Disease. Am. J. Pathol. 159: 1807-1814 [Abstract] [Full Text]  
  • Anderson, M., Hernandez-Hoyos, G, Diamond, R., Rothenberg, E. (1999). Precise developmental regulation of Ets family transcription factors during specification and commitment to the T cell lineage. Development 126: 3131-3148 [Abstract]  
  • NIKOLAJCZYK, B.S., DANG, W., SEN, R. (1999). Mechanisms of {micro} Enhancer Regulation in B Lymphocytes. Cold Spring Harb Symp Quant Biol 64: 99-108 [Abstract]  
  • Wang, Z., Goldstein, A., Zong, R.-T., Lin, D., Neufeld, E. J., Scheuermann, R. H., Tucker, P. W. (1999). Cux/CDP Homeoprotein Is a Component of NF-µNR and Represses the Immunoglobulin Heavy Chain Intronic Enhancer by Antagonizing the Bright Transcription Activator. Mol. Cell. Biol. 19: 284-295 [Abstract] [Full Text]  
  • WEBB, C., ZONG, R.-T., LIN, D., WANG, Z., KAPLAN, M., PAULIN, Y., SMITH, E., PROBST, L., BRYANT, J., GOLDSTEIN, A., SCHEUERMANN, R., TUCKER, P. (1999). Differential Regulation of Immunoglobulin Gene Transcription via Nuclear Matrix-associated Regions. Cold Spring Harb Symp Quant Biol 64: 109-118 [Abstract]  
  • Nagulapalli, S., Atchison, M. L. (1998). Transcription Factor Pip Can Enhance DNA Binding by E47, Leading to Transcriptional Synergy Involving Multiple Protein Domains. Mol. Cell. Biol. 18: 4639-4650 [Abstract] [Full Text]  
  • Perkel, J. M., Atchison, M. L. (1998). A Two-Step Mechanism for Recruitment of Pip by PU.1. J. Immunol. 160: 241-252 [Abstract] [Full Text]  
  • Rao, E., Dang, W., Tian, G., Sen, R. (1997). A Three-protein-DNA Complex on a B Cell-specific Domain of the ImmunoglobulinµHeavy Chain Gene Enhancer. J. Biol. Chem. 272: 6722-6732 [Abstract] [Full Text]  
  • Akbarali, Y., Oettgen, P., Boltax, J., Libermann, T. A. (1996). ELF-1 Interacts with and Transactivates the IgH Enhancer pi Site. J. Biol. Chem. 271: 26007-26012 [Abstract] [Full Text]  
  • Chiaramello, A., Neuman, T., Peavy, D. R., Zuber, M. X. (1996). The GAP-43 Gene Is a Direct Downstream Target of the Basic Helix-Loop-Helix Transcription Factors. J. Biol. Chem. 271: 22035-22043 [Abstract] [Full Text]  
  • Herrscher, R F, Kaplan, M H, Lelsz, D L, Das, C, Scheuermann, R, Tucker, P W (1995). The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family.. Genes Dev. 9: 3067-3082 [Abstract]  
  • Rosmarin, A. G., Caprio, D. G., Kirsch, D. G., Handa, H., Simkevich, C. P. (1995). GABP and PU.1 Compete for Binding, yet Cooperate to Increase CD18 (beta(2) Leukocyte Integrin) Transcription. J. Biol. Chem. 270: 23627-23633 [Abstract] [Full Text]  
  • Eklund, E. A., Skalnik, D. G. (1995). Characterization of a gp91-phox Promoter Element That Is Required for Interferon [IMAGE]-induced Transcription. J. Biol. Chem. 270: 8267-8273 [Abstract] [Full Text]  
  • Schwarzenbach, H., Newell, J. W., Matthias, P. (1995). Involvement of the Ets Family Factor PU.1 in the Activation of Immunoglobulin Promoters. J. Biol. Chem. 270: 898-907 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»