This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, J Articles by Liang, T J Search for Related Content PubMed PubMed Citation Articles by Huang, J Articles by Liang, T J

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 December; 13(12): 7476-7486

A novel hepatitis B virus (HBV) genetic element with Rev response element-like properties that is essential for expression of HBV gene products.

Gastrointestinal Unit, Massachusetts General Hospital, Boston.

ABSTRACT

Many viruses possess complex mechanisms involving multiple gene products and cis-regulatory elements in order to achieve a fine control of their gene expression at both transcriptional and posttranscriptional levels. Hepatitis B virus (HBV) and retroviruses share many structural and functional similarities. In this study, by genetic and biochemical analyses, we have demonstrated the existence of a novel genetic element within the HBV genome which is essential for high-level expression of viral gene products. This element is located 3' to the envelope coding region. We have shown that this genetic element is cis acting at the posttranscriptional level and that its function is exerted at the level of RNA processing as part of transcribed sequences. This RNA element is also functional in the context of a heterologous gene. Similar to the function of Rev-Rev response element interaction of human immunodeficiency virus type 1, this element appears to inhibit the splicing process and facilitate the transport and utilization of HBV transcripts.

This article has been cited by other articles:

• Schwalbe, M., Ohlenschlager, O., Marchanka, A., Ramachandran, R., Hafner, S., Heise, T., Gorlach, M. (2008). Solution structure of stem-loop {alpha} of the hepatitis B virus post-transcriptional regulatory element. Nucleic Acids Res 36: 1681-1689 [Abstract] [Full Text]  
• Heise, T., Sommer, G., Reumann, K., Meyer, I., Will, H., Schaal, H. (2006). The hepatitis B virus PRE contains a splicing regulatory element. Nucleic Acids Res 34: 353-363 [Abstract] [Full Text]  
• Huang, T.-J., Lu, C.-C., Tsai, J.-C., Yao, W.-J., Lu, X., Lai, M.-D., Liu, H.-S., Shiau, A.-L. (2005). Novel Autoregulatory Function of Hepatitis B Virus M Protein on Surface Gene Expression. J. Biol. Chem. 280: 27742-27754 [Abstract] [Full Text]  
• Wolff, H., Brack-Werner, R., Neumann, M., Werner, T., Schneider, R. (2003). Integrated functional and bioinformatics approach for the identification and experimental verification of RNA signals: application to HIV-1 INS. Nucleic Acids Res 31: 2839-2851 [Abstract] [Full Text]  
• LU, S., CULLEN, B. R. (2003). Analysis of the stimulatory effect of splicing on mRNA production and utilization in mammalian cells. RNA 9: 618-630 [Abstract] [Full Text]  
• Popa, I., Harris, M. E., Donello, J. E., Hope, T. J. (2002). CRM1-Dependent Function of a cis-Acting RNA Export Element. Mol. Cell. Biol. 22: 2057-2067 [Abstract] [Full Text]  
• Rang, A., Bruns, M., Heise, T., Will, H. (2002). Antiviral Activity of Interferon-alpha against Hepatitis B Virus Can Be Studied in Non-hepatic Cells and Is Independent of MxA. J. Biol. Chem. 277: 7645-7647 [Abstract] [Full Text]  
• Wodrich, H., Bohne, J., Gumz, E., Welker, R., Krausslich, H.-G. (2001). A New RNA Element Located in the Coding Region of a Murine Endogenous Retrovirus Can Functionally Replace the Rev/Rev-Responsive Element System in Human Immunodeficiency Virus Type 1 Gag Expression. J. Virol. 75: 10670-10682 [Abstract] [Full Text]  
• Zang, W.-Q., Li, B., Huang, P.-Y., Lai, M. M. C., Yen, T. S. B. (2001). Role of Polypyrimidine Tract Binding Protein in the Function of the Hepatitis B Virus Posttranscriptional Regulatory Element. J. Virol. 75: 10779-10786 [Abstract] [Full Text]  
• DAS, M., HARVEY, I., CHU, L. L., SINHA, M., PELLETIER, J. (2001). Full-length cDNAs: more than just reaching the ends. Physiol. Genomics 6: 57-80 [Abstract] [Full Text]  
• Ruvolo, V., Gupta, A. K., Swaminathan, S. (2001). Epstein-Barr Virus SM Protein Interacts with mRNA In Vivo and Mediates a Gene-Specific Increase in Cytoplasmic mRNA. J. Virol. 75: 6033-6041 [Abstract] [Full Text]  
• Gordien, E., Rosmorduc, O., Peltekian, C., Garreau, F., Bréchot, C., Kremsdorf, D. (2001). Inhibition of Hepatitis B Virus Replication by the Interferon-Inducible MxA Protein. J. Virol. 75: 2684-2691 [Abstract] [Full Text]  
• Buck, C. B., Shen, X., Egan, M. A., Pierson, T. C., Walker, C. M., Siliciano, R. F. (2001). The Human Immunodeficiency Virus Type 1 gag Gene Encodes an Internal Ribosome Entry Site. J. Virol. 75: 181-191 [Abstract] [Full Text]  
• Guo, W., Winistorfer, S. C., Stoltzfus, C. M. (2000). Selective Inhibition of Splicing at the Avian Sarcoma Virus src 3' Splice Site by Direct-Repeat Posttranscriptional cis Elements. J. Virol. 74: 8513-8523 [Abstract] [Full Text]  
• Jehle, C., Lipkin, W. I., Staeheli, P., Marion, R. M., Schwemmle, M. (2000). Authentic Borna disease virus transcripts are spliced less efficiently than cDNA-derived viral RNAs. J. Gen. Virol. 81: 1947-1954 [Abstract] [Full Text]  
• zur Megede, J., Chen, M.-C., Doe, B., Schaefer, M., Greer, C. E., Selby, M., Otten, G. R., Barnett, S. W. (2000). Increased Expression and Immunogenicity of Sequence-Modified Human Immunodeficiency Virus Type 1 gag Gene. J. Virol. 74: 2628-2635 [Abstract] [Full Text]  
• Weigel, S., Dobbelstein, M. (2000). The Nuclear Export Signal within the E4orf6 Protein of Adenovirus Type 5 Supports Virus Replication and Cytoplasmic Accumulation of Viral mRNA. J. Virol. 74: 764-772 [Abstract] [Full Text]  
• Wagner, M., Alt, M., Hofschneider, P. H., Renner, M. (1999). A novel negative cis-regulatory element on the hepatitis B virus S-(+)-strand. J. Gen. Virol. 80: 2673-2683 [Abstract] [Full Text]  
• Protzer, U., Nassal, M., Chiang, P.-W., Kirschfink, M., Schaller, H. (1999). Interferon gene transfer by a hepatitis B virus vector efficiently suppresses wild-type virus infection. Proc. Natl. Acad. Sci. USA 96: 10818-10823 [Abstract] [Full Text]  
• Heise, T., Guidotti, L. G., Chisari, F. V. (1999). La Autoantigen Specifically Recognizes a Predicted Stem-Loop in Hepatitis B Virus RNA. J. Virol. 73: 5767-5776 [Abstract] [Full Text]  
• Zhao, J., Hyman, L., Moore, C. (1999). Formation of mRNA 3' Ends in Eukaryotes: Mechanism, Regulation, and Interrelationships with Other Steps in mRNA Synthesis. Microbiol. Mol. Biol. Rev. 63: 405-445 [Abstract] [Full Text]  
• Zufferey, R., Donello, J. E., Trono, D., Hope, T. J. (1999). Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances Expression of Transgenes Delivered by Retroviral Vectors. J. Virol. 73: 2886-2892 [Abstract] [Full Text]  
• Li, J., Tang, H., Mullen, T.-M., Westberg, C., Reddy, T. R., Rose, D. W., Wong-Staal, F. (1999). A role for RNA helicase A in post-transcriptional regulation of HIV type 1. Proc. Natl. Acad. Sci. USA 96: 709-714 [Abstract] [Full Text]  
• Heise, T., Guidotti, L. G., Cavanaugh, V. J., Chisari, F. V. (1999). Hepatitis B Virus RNA-Binding Proteins Associated with Cytokine-Induced Clearance of Viral RNA from the Liver of Transgenic Mice. J. Virol. 73: 474-481 [Abstract] [Full Text]  
• Otero, G. C., Hope, T. J. (1998). Splicing-Independent Expression of the Herpes Simplex Virus Type 1 Thymidine Kinase Gene Is Mediated by Three cis-Acting RNA Subelements. J. Virol. 72: 9889-9896 [Abstract] [Full Text]  
• Otero, G. C., Harris, M. E., Donello, J. E., Hope, T. J. (1998). Leptomycin B Inhibits Equine Infectious Anemia Virus Rev and Feline Immunodeficiency Virus Rev Function but Not the Function of the Hepatitis B Virus Posttranscriptional Regulatory Element. J. Virol. 72: 7593-7597 [Abstract] [Full Text]  
• Heinkelein, M., Schmidt, M., Fischer, N., Moebes, A., Lindemann, D., Enssle, J., Rethwilm, A. (1998). Characterization of a cis-Acting Sequence in the pol Region Required To Transfer Human Foamy Virus Vectors. J. Virol. 72: 6307-6314 [Abstract] [Full Text]  
• Baumert, T. F., Marrone, A., Vergalla, J., Liang, T. J. (1998). Naturally Occurring Mutations Define a Novel Function of the Hepatitis B Virus Core Promoter in Core Protein Expression. J. Virol. 72: 6785-6795 [Abstract] [Full Text]  
• Donello, J. E., Loeb, J. E., Hope, T. J. (1998). Woodchuck Hepatitis Virus Contains a Tripartite Posttranscriptional Regulatory Element. J. Virol. 72: 5085-5092 [Abstract] [Full Text]  
• Huang, Y., Carmichael, G. G. (1997). The mouse histone H2a gene contains a small element that facilitates cytoplasmic accumulation of intronless gene transcripts and of unspliced HIV-1-related mRNAs. Proc. Natl. Acad. Sci. USA 94: 10104-10109 [Abstract] [Full Text]  
• Liu, X, Mertz, J E (1995). HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.. Genes Dev. 9: 1766-1780 [Abstract]