This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Muraosa, Y
Right arrow Articles by Shibahara, S
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muraosa, Y
Right arrow Articles by Shibahara, S

 Previous Article  |  Next Article 

Mol Cell Biol. 1993 December; 13(12): 7881-7891

Identification of a cis-regulatory element and putative trans-acting factors responsible for 12-O-tetradecanoylphorbol-13-acetate (TPA)-mediated induction of heme oxygenase expression in myelomonocytic cell lines.

Y Muraosa and S Shibahara

Department of Applied Physiology and Molecular Biology, Tohoku University School of Medicine, Miyagi, Japan.

ABSTRACT

The human monocytic leukemia cell line THP-1 differentiates into macrophage-like cells when treated with a variety of agents, including 12-O-tetradecanoylphorbol-13-acetate (TPA). We show here that during this process, the expression of heme oxygenase, a rate-limiting enzyme in heme catabolism, is induced. Treatment with TPA increases heme oxygenase mRNA in other myelomonocytic cell lines also, but not in cell lines of other lineages, such as HeLa cells. Increased heme oxygenase activity may represent one of the functions of activated macrophages, which sequestrate senescent erythrocytes and degrade heme derived from hemoglobin. This cell-type-specific induction by TPA treatment further investigated with respect to transcriptional regulation. We defined a cis-regulatory element, 5'-GTCATATGAC-3', located in the 5'-flanking region (positions -156 to -147) of the human heme oxygenase gene, which confers inducibility by TPA in THP-1 cells but not in HeLa cells. Nuclear proteins that bind to this element, which may be responsible for the cell specificity, were identified in THP-1 nuclear extracts. This element contains the consensus motif CANNTG, to which a large family of basic helix-loop-helix proteins binds. Our results suggest a novel mechanism of TPA-mediated transcriptional regulation in myelomonocytic cell lines.

Mol Cell Biol. 1993 December; 13(12): 7881-7891




This article has been cited by other articles:

  • Naidu, S., Wijayanti, N., Santoso, S., Kietzmann, T., Immenschuh, S. (2008). An Atypical NF-{kappa}B-Regulated Pathway Mediates Phorbol Ester-Dependent Heme Oxygenase-1 Gene Activation in Monocytes. J. Immunol. 181: 4113-4123 [Abstract] [Full Text]  
  • Ryter, S. W., Alam, J., Choi, A. M. K. (2006). Heme Oxygenase-1/Carbon Monoxide: From Basic Science to Therapeutic Applications. Physiol. Rev. 86: 583-650 [Abstract] [Full Text]  
  • Wijayanti, N., Kietzmann, T., Immenschuh, S. (2005). Heme Oxygenase-1 Gene Activation by the NAD(P)H Oxidase Inhibitor 4-(2-Aminoethyl) Benzenesulfonyl Fluoride via a Protein Kinase B, p38-dependent Signaling Pathway in Monocytes. J. Biol. Chem. 280: 21820-21829 [Abstract] [Full Text]  
  • Ricchetti, G. A., Williams, L. M., Foxwell, B. M. J. (2004). Heme oxygenase 1 expression induced by IL-10 requires STAT-3 and phosphoinositol-3 kinase and is inhibited by lipopolysaccharide. J. Leukoc. Biol. 76: 719-726 [Abstract] [Full Text]  
  • Kitamuro, T., Takahashi, K., Ogawa, K., Udono-Fujimori, R., Takeda, K., Furuyama, K., Nakayama, M., Sun, J., Fujita, H., Hida, W., Hattori, T., Shirato, K., Igarashi, K., Shibahara, S. (2003). Bach1 Functions as a Hypoxia-inducible Repressor for the Heme Oxygenase-1 Gene in Human Cells. J. Biol. Chem. 278: 9125-9133 [Abstract] [Full Text]  
  • Immenschuh, S., Hinke, V., Katz, N., Kietzmann, T. (2000). Transcriptional Induction of Heme Oxygenase-1 Gene Expression by Okadaic Acid in Primary Rat Hepatocyte Cultures. Mol. Pharmacol. 57: 610-618 [Abstract] [Full Text]  
  • Nishie, A., Ono, M., Shono, T., Fukushi, J., Otsubo, M., Onoue, H., Ito, Y., Inamura, T., Ikezaki, K., Fukui, M., Iwaki, T., Kuwano, M. (1999). Macrophage Infiltration and Heme Oxygenase-1 Expression Correlate with Angiogenesis in Human Gliomas. Clin. Cancer Res. 5: 1107-1113 [Abstract] [Full Text]  
  • Terry, C. M., Clikeman, J. A., Hoidal, J. R., Callahan, K. S. (1999). TNF-alpha and IL-1alpha induce heme oxygenase-1 via protein kinase C, Ca2+, and phospholipase A2 in endothelial cells. Am. J. Physiol. Heart Circ. Physiol. 276: H1493-H1501 [Abstract] [Full Text]  
  • Immenschuh, S., Kietzmann, T., Hinke, V., Wiederhold, M., Katz, N., Muller-Eberhard, U. (1998). The Rat Heme Oxygenase-1 Gene Is Transcriptionally Induced via the Protein Kinase A Signaling Pathway in Rat Hepatocyte Cultures. Mol. Pharmacol. 53: 483-491 [Abstract] [Full Text]  
  • Xie, M., Shao, G., Buyse, I. M., Huang, S. (1997). Transcriptional Repression Mediated by the PR Domain Zinc Finger Gene RIZ. J. Biol. Chem. 272: 26360-26366 [Abstract] [Full Text]  
  • Yasumoto, K.-i., Yokoyama, K., Takahashi, K., Tomita, Y., Shibahara, S. (1997). Functional Analysis of Microphthalmia-associated Transcription Factor in Pigment Cell-specific Transcription of the Human Tyrosinase Family Genes. J. Biol. Chem. 272: 503-509 [Abstract] [Full Text]  
  • Alam, J., Camhi, S., Choi, A. M. K. (1995). Identification of a Second Region Upstream of the Mouse Heme Oxygenase-1 Gene That Functions as a Basal Level and Inducer-dependent Transcription Enhancer. J. Biol. Chem. 270: 11977-11984 [Abstract] [Full Text]  
  • Weinziger, R., Salgado, L. M., David, C. N., Bosch, T. C. (1994). Ks1, an epithelial cell-specific gene, responds to early signals of head formation in Hydra. Development 120: 2511-2517 [Abstract]  
  • Schick, B. P., Petrushina, I., Brodbeck, K. C., Castronuevo, P. (2001). Promoter Regulatory Elements and DNase I-hypersensitive Sites Involved in Serglycin Proteoglycan Gene Expression in Human Erythroleukemia, CHRF 288-11, and HL-60 Cells. J. Biol. Chem. 276: 24726-24735 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»