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Mol Cell Biol. 1993 December; 13(12): 7913-7924

The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiae.

J R Geiser, H A Sundberg, B H Chang, E G Muller and T N Davis

Department of Biochemistry, University of Washington, Seattle 98195.

ABSTRACT

Two independent methods identified the spindle pole body component Nuf1p/Spc110p as the essential mitotic target of calmodulin. Extragenic suppressors of cmd1-1 were isolated and found to define three loci, XCM1, XCM2, and XCM3 (extragenic suppressor of cmd1-1). The gene encoding a dominant suppressor allele of XCM1 was cloned. On the basis of DNA sequence analysis, genetic cosegregation, and mutational analysis, XCM1 was identified as NUF1/SPC110. Independently, a C-terminal portion of Nuf1p/Spc110p, amino acid residues 828 to 944, was isolated as a calmodulin-binding protein by the two-hybrid system. As assayed by the two-hybrid system, Nuf1p/Spc110p interacts with wild-type calmodulin and triple-mutant calmodulins defective in binding Ca2+ but not with two mutant calmodulins that confer a temperature-sensitive phenotype. Deletion analysis by the two-hybrid system mapped the calmodulin-binding site of Nuf1p/Spc110p to amino acid residues 900 to 927. Direct binding between calmodulin and Nuf1p/Spc110p was demonstrated by a modified gel overlay assay. Furthermore, indirect immunofluorescence with fixation procedures known to aid visualization of spindle pole body components localized calmodulin to the spindle pole body. Sequence analysis of five suppressor alleles of NUF1/SPC110 indicated that suppression of cmd1-1 occurs by C-terminal truncation of Nuf1p/Spc110p at amino acid residues 856, 863, or 881, thereby removing the calmodulin-binding site.

Mol Cell Biol. 1993 December; 13(12): 7913-7924




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  • Moser, M., Flory, M., Davis, T. (1997). Calmodulin localizes to the spindle pole body of Schizosaccharomyces pombe and performs an essential function in chromosome segregation. J. Cell Sci. 110: 1805-1812 [Abstract]  
  • Zarzov, P, Boucherie, H, Mann, C (1997). A yeast heat shock transcription factor (Hsf1) mutant is defective in both Hsc82/Hsp82 synthesis and spindle pole body duplication. J. Cell Sci. 110: 1879-1891 [Abstract]  
  • Melcher, M. L., Thorner, J. (1996). Identification and Characterization of the CLK1 Gene Product, a Novel CaM Kinase-like Protein Kinase from the Yeast Saccharomyces cerevisiae. J. Biol. Chem. 271: 29958-29968 [Abstract] [Full Text]  
  • Geier, B. M., Wiech, H., Schiebel, E. (1996). Binding of Centrins and Yeast Calmodulin to Synthetic Peptides Corresponding to Binding Sites in the Spindle Pole Body Components Kar1p and Spc110p. J. Biol. Chem. 271: 28366-28374 [Abstract] [Full Text]  
  • Wiech, H., Geier, B. M., Paschke, T., Spang, A., Grein, K., Steinkotter, J., Melkonian, M., Schiebel, E. (1996). Characterization of Green Alga, Yeast, and Human Centrins. SPECIFIC SUBDOMAIN FEATURES DETERMINE FUNCTIONAL DIVERSITY. J. Biol. Chem. 271: 22453-22461 [Abstract] [Full Text]  
  • Stirling, D., Rayner, T., Prescott, A., Stark, M. (1996). Mutations which block the binding of calmodulin to Spc110p cause multiple mitotic defects. J. Cell Sci. 109: 1297-1310 [Abstract]  
  • Moser, M. J., Lee, S. Y., Klevit, R. E., Davis, T. N. (1995). Ca[IMAGE] Binding to Calmodulin and Its Role in Schizosaccharomyces pombe as Revealed by Mutagenesis and NMR Spectroscopy. J. Biol. Chem. 270: 20643-20652 [Abstract] [Full Text]  
  • Harris, E, Watterson, D., Thorner, J (1994). Functional consequences in yeast of single-residue alterations in a consensus calmodulin. J. Cell Sci. 107: 3235-3249 [Abstract]  
  • Friedman, D. B., Kern, J. W., Huneycutt, B. J., Vinh, D. B. N., Crawford, D. K., Steiner, E., Scheiltz, D., Yates, J. III, Resing, K. A., Ahn, N. G., Winey, M., Davis, T. N. (2001). Yeast Mps1p Phosphorylates the Spindle Pole Component Spc110p in the N-terminal Domain. J. Biol. Chem. 276: 17958-17967 [Abstract] [Full Text]  


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